Reflection Phase Microscopy by Successive Accumulation of Interferograms

Min Gyu Hyeon, Taeseok Daniel Yang, Jin Sung Park, Kwanjun Park, Yong Guk Kang, Beop-Min Kim, Youngwoon Choi

Research output: Contribution to journalArticle

Abstract

Imaging three-dimensional (3-D) structures of biological specimens without exogenous contrast agents is desired in biological and medical science in order not to disturb the physiological status of the living samples. Reflection phase microscopy based on interferometric detection has been useful for the label-free observation of such samples. However, the achievement of optical sectioning has been mainly based on the time gating set by the broad spectra of light sources. Here we propose wide-field reflection phase microscopy using a light source of narrow bandwidth, which is yet capable of achieving the optical sectioning sufficient for 3-D imaging of biological specimens. The depth selectivity is achieved by successive accumulation of interferograms (SAI) produced by synchronous angular scanning of a plane wave on both the sample and reference planes. This intensity-based cumulative process eventually results in a coherent addition of object fields that quickly attenuates the out-of-focus information along the axial direction. We theoretically investigated and numerically verified the generation of the depth selectivity by SAI. We also implemented a reflection phase microscope working with this principle and then demonstrated high-resolution 3-D imaging of living cells and small worms in a label-free manner.

Original languageEnglish
JournalACS Photonics
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Three-Dimensional Imaging
Microscopy
Microscopic examination
interferometry
microscopy
Imaging techniques
Light sources
Labels
light sources
selectivity
medical science
Light
worms
Biological Science Disciplines
Contrast Media
plane waves
Microscopes
microscopes
Cells
Observation

Keywords

  • 3-D imaging
  • cellular membrane fluctuation
  • depth selectivity
  • live cell imaging
  • quantitative phase imaging
  • synchronous angular scanning

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biotechnology
  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

Cite this

Reflection Phase Microscopy by Successive Accumulation of Interferograms. / Hyeon, Min Gyu; Yang, Taeseok Daniel; Park, Jin Sung; Park, Kwanjun; Kang, Yong Guk; Kim, Beop-Min; Choi, Youngwoon.

In: ACS Photonics, 01.01.2019.

Research output: Contribution to journalArticle

Hyeon, Min Gyu ; Yang, Taeseok Daniel ; Park, Jin Sung ; Park, Kwanjun ; Kang, Yong Guk ; Kim, Beop-Min ; Choi, Youngwoon. / Reflection Phase Microscopy by Successive Accumulation of Interferograms. In: ACS Photonics. 2019.
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