Reflection phase microscopy using spatio-temporal coherence of light

Youngwoon Choi, Poorya Hosseini, Jeon Woong Kang, Sungsam Kang, Taeseok Daniel Yang, Min Gyu Hyeon, Beop-Min Kim, Peter T.C. So, Zahid Yaqoob

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3 Citations (Scopus)

Abstract

Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes.

Original languageEnglish
Pages (from-to)1468-1473
Number of pages6
JournalOptica
Volume5
Issue number11
DOIs
Publication statusPublished - 2018 Nov 20

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Choi, Y., Hosseini, P., Kang, J. W., Kang, S., Yang, T. D., Hyeon, M. G., Kim, B-M., So, P. T. C., & Yaqoob, Z. (2018). Reflection phase microscopy using spatio-temporal coherence of light. Optica, 5(11), 1468-1473. https://doi.org/10.1364/OPTICA.5.001468