Abstract
Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes.
| Original language | English |
|---|---|
| Pages (from-to) | 1468-1473 |
| Number of pages | 6 |
| Journal | Optica |
| Volume | 5 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2018 Nov 20 |
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ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
Cite this
Reflection phase microscopy using spatio-temporal coherence of light. / Choi, Youngwoon; Hosseini, Poorya; Kang, Jeon Woong; Kang, Sungsam; Yang, Taeseok Daniel; Hyeon, Min Gyu; Kim, Beop-Min; So, Peter T.C.; Yaqoob, Zahid.
In: Optica, Vol. 5, No. 11, 20.11.2018, p. 1468-1473.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reflection phase microscopy using spatio-temporal coherence of light
AU - Choi, Youngwoon
AU - Hosseini, Poorya
AU - Kang, Jeon Woong
AU - Kang, Sungsam
AU - Yang, Taeseok Daniel
AU - Hyeon, Min Gyu
AU - Kim, Beop-Min
AU - So, Peter T.C.
AU - Yaqoob, Zahid
PY - 2018/11/20
Y1 - 2018/11/20
N2 - Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes.
AB - Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes.
UR - http://www.scopus.com/inward/record.url?scp=85059028205&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85059028205&partnerID=8YFLogxK
U2 - 10.1364/OPTICA.5.001468
DO - 10.1364/OPTICA.5.001468
M3 - Article
AN - SCOPUS:85059028205
VL - 5
SP - 1468
EP - 1473
JO - Optica
JF - Optica
SN - 2334-2536
IS - 11
ER -