Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum

YongKeun Park, Monica Diez-Silva, Gabriel Popescu, George Lykotrafitis, Wonshik Choi, Michael S. Feld, Subra Suresh

Research output: Contribution to journalArticle

439 Citations (Scopus)

Abstract

Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.

Original languageEnglish
Pages (from-to)13730-13735
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number37
DOIs
Publication statusPublished - 2008 Sep 16
Externally publishedYes

Fingerprint

Refractometry
Plasmodium falciparum
Erythrocytes
Membranes
Microscopy
Cell Membrane
Falciparum Malaria
Parasites
Fever
Temperature

Keywords

  • Erythrocyte
  • Febrile temperature
  • Malaria
  • Mechanical properties
  • Optical techniques

ASJC Scopus subject areas

  • General

Cite this

Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum. / Park, YongKeun; Diez-Silva, Monica; Popescu, Gabriel; Lykotrafitis, George; Choi, Wonshik; Feld, Michael S.; Suresh, Subra.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 37, 16.09.2008, p. 13730-13735.

Research output: Contribution to journalArticle

Park, YongKeun ; Diez-Silva, Monica ; Popescu, Gabriel ; Lykotrafitis, George ; Choi, Wonshik ; Feld, Michael S. ; Suresh, Subra. / Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 37. pp. 13730-13735.
@article{13bab38e7352464fa07b93e0e7540637,
title = "Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum",
abstract = "Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.",
keywords = "Erythrocyte, Febrile temperature, Malaria, Mechanical properties, Optical techniques",
author = "YongKeun Park and Monica Diez-Silva and Gabriel Popescu and George Lykotrafitis and Wonshik Choi and Feld, {Michael S.} and Subra Suresh",
year = "2008",
month = "9",
day = "16",
doi = "10.1073/pnas.0806100105",
language = "English",
volume = "105",
pages = "13730--13735",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "37",

}

TY - JOUR

T1 - Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum

AU - Park, YongKeun

AU - Diez-Silva, Monica

AU - Popescu, Gabriel

AU - Lykotrafitis, George

AU - Choi, Wonshik

AU - Feld, Michael S.

AU - Suresh, Subra

PY - 2008/9/16

Y1 - 2008/9/16

N2 - Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.

AB - Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.

KW - Erythrocyte

KW - Febrile temperature

KW - Malaria

KW - Mechanical properties

KW - Optical techniques

UR - http://www.scopus.com/inward/record.url?scp=52949115297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52949115297&partnerID=8YFLogxK

U2 - 10.1073/pnas.0806100105

DO - 10.1073/pnas.0806100105

M3 - Article

C2 - 18772382

AN - SCOPUS:52949115297

VL - 105

SP - 13730

EP - 13735

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 37

ER -