Regeneration of Achilles' Tendon

The role of dynamic stimulation for enhanced cell proliferation and mechanical properties

Jongman Lee, Vincenzo Guarino, Antonio Gloria, Luigi Ambrosio, Giyoong Tae, Young Ha Kim, Youngmee Jung, Sang Heon Kim, Soo Hyun Kim

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The tissue engineering of tendon was studied using highly elastic poly(L-lactide-co-ε-caprolactone) (PLCL) scaffolds and focusing on the effect of dynamic tensile stimulation. Tenocytes from rabbit Achilles tendon were seeded (1.0 × 106 cells/scaffold) onto porous PLCL scaffolds and cultured for periods of 2 weeks and 4 weeks. This was performed in a static system and also in a bioreactor equipped with tensile modulation which mimicked the environmental surroundings of tendons with respect to tensile extension. The degradation of the polymeric scaffolds during the culture was relatively slow. However, there was an indication that cells accelerated the degradation of PLCL scaffolds. The scaffold/cell adducts from the static culture exhibited inferior strength (at 2 weeks 350 kPa, 4 weeks 300 kPa) compared to the control without cells (at 2 weeks 460 kPa, 4 weeks 340 kPa), indicating that the cells contributed to the enhanced degradation. On the contrary, the corresponding values of the adducts from the dynamic culture (at 2 weeks 430 kPa, 4 weeks 370 kPa) were similar to, or higher than, those from the control. This could be explained by the increased quantity of cells and neo-tissues in the case of dynamic culture compensating for the loss in tensile strength. Compared with static and dynamic culture conditions, mechanical stimulation played a crucial role in the regeneration of tendon tissue. In the case of the dynamic culture system, cell proliferation was enhanced and secretion of collagen type I was increased, as evidenced by DNA assay and histological and immunofluorescence analysis. Thus, tendon regeneration, indicated by improved mechanical and biological properties, was demonstrated, confirming the effect of mechanical stimulation. It could be concluded that the dynamic tensile stimulation appeared to be an essential factor in tendon/ligament tissue engineering, and that elastic PLCL co-polymers could be very beneficial in this process.

Original languageEnglish
Pages (from-to)1173-1190
Number of pages18
JournalJournal of Biomaterials Science, Polymer Edition
Volume21
Issue number8-9
DOIs
Publication statusPublished - 2010 May 1
Externally publishedYes

Fingerprint

Achilles Tendon
Tendons
Cell proliferation
Scaffolds
Regeneration
Cell Proliferation
Mechanical properties
Tissue Engineering
Tissue engineering
Degradation
Tissue
Tensile Strength
Ligaments
Bioreactors
Collagen Type I
Cell culture
Collagen
Fluorescent Antibody Technique
Assays
Polymers

Keywords

  • BIOREACTOR
  • MECHANICAL STIMULATION
  • PLCL
  • TENDON
  • TISSUE ENGINEERING

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

Regeneration of Achilles' Tendon : The role of dynamic stimulation for enhanced cell proliferation and mechanical properties. / Lee, Jongman; Guarino, Vincenzo; Gloria, Antonio; Ambrosio, Luigi; Tae, Giyoong; Kim, Young Ha; Jung, Youngmee; Kim, Sang Heon; Kim, Soo Hyun.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 21, No. 8-9, 01.05.2010, p. 1173-1190.

Research output: Contribution to journalArticle

Lee, Jongman ; Guarino, Vincenzo ; Gloria, Antonio ; Ambrosio, Luigi ; Tae, Giyoong ; Kim, Young Ha ; Jung, Youngmee ; Kim, Sang Heon ; Kim, Soo Hyun. / Regeneration of Achilles' Tendon : The role of dynamic stimulation for enhanced cell proliferation and mechanical properties. In: Journal of Biomaterials Science, Polymer Edition. 2010 ; Vol. 21, No. 8-9. pp. 1173-1190.
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