Regional difference in colonic motility response to electrical field stimulation in guinea pig

Jung-Myun Kwak, Reji Babygirija, Irena Gribovskaja-Rupp, Toku Takahashi, Shigeru Yamato, Kirk Ludwig

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background/Aims In isolated guinea-pig colon, we investigated regional differences in peristalsis evoked by intrinsic electrical nerve stimulation. Methods Four colonic segments from mid and distal colon of Hartley guinea pigs, were mounted horizontally in an organ bath. Measurement of pellet propulsion time, intraluminal pressure, electrical field stimulation (EFS; 0.5 ms, 60 V, 10 Hz), and response of pharmacological antagonists, were performed to isolated segments of colon to determine the mechanisms underlying peristaltic reflexes evoked by focal electrical nerve stimuli. Results In fecal pellet propulsion study, the velocity of pellet propulsion was significantly faster in the distal colon and decreased gradually to the proximal part of the mid colon. Intraluminal pressure recording studies showed that luminal infusion initiated normal peristaltic contractions (PCs) in 82% trials of the distal colon, compared to that of mid colon. In response to EFS, the incidence of PCs was significantly increased in the distal colon in contrast, the incidence of non-peristaltic contractions (NPCs) was significantly higher in the middle-mid colon, distal-mid colon and distal colon, compared to that of proximal-mid colon. Addition of L-NAME into the bath increased the frequency of NPCs. EFS failed to cause any PCs or NPCs contractions in the presence of hexamethonium, atropine or tetrodotoxin. Conclusions This study has revealed that electrical nerve stimulation of distal colon is the most likely region to elicit a peristaltic wave, com - pared with the mid or proximal colon. Our findings suggest that EFS-evoked PCs can be modulated by endogenous nitric oxide.

Original languageEnglish
Pages (from-to)192-203
Number of pages12
JournalJournal of Neurogastroenterology and Motility
Volume19
Issue number2
DOIs
Publication statusPublished - 2013 Apr 1

Fingerprint

Electric Stimulation
Guinea Pigs
Colon
Baths
Pressure
Peristalsis
Hexamethonium
NG-Nitroarginine Methyl Ester
Incidence
Tetrodotoxin
Atropine
Reflex
Nitric Oxide
Pharmacology

Keywords

  • Anti-peristalsis
  • Colonic transit
  • Nitric oxide

ASJC Scopus subject areas

  • Clinical Neurology
  • Gastroenterology

Cite this

Regional difference in colonic motility response to electrical field stimulation in guinea pig. / Kwak, Jung-Myun; Babygirija, Reji; Gribovskaja-Rupp, Irena; Takahashi, Toku; Yamato, Shigeru; Ludwig, Kirk.

In: Journal of Neurogastroenterology and Motility, Vol. 19, No. 2, 01.04.2013, p. 192-203.

Research output: Contribution to journalArticle

Kwak, Jung-Myun ; Babygirija, Reji ; Gribovskaja-Rupp, Irena ; Takahashi, Toku ; Yamato, Shigeru ; Ludwig, Kirk. / Regional difference in colonic motility response to electrical field stimulation in guinea pig. In: Journal of Neurogastroenterology and Motility. 2013 ; Vol. 19, No. 2. pp. 192-203.
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