Regional difference in colonic motility response to electrical field stimulation in guinea pig

Jung Myun Kwak, Reji Babygirija, Irena Gribovskaja-Rupp, Toku Takahashi, Shigeru Yamato, Kirk Ludwig

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    Background/Aims In isolated guinea-pig colon, we investigated regional differences in peristalsis evoked by intrinsic electrical nerve stimulation. Methods Four colonic segments from mid and distal colon of Hartley guinea pigs, were mounted horizontally in an organ bath. Measurement of pellet propulsion time, intraluminal pressure, electrical field stimulation (EFS; 0.5 ms, 60 V, 10 Hz), and response of pharmacological antagonists, were performed to isolated segments of colon to determine the mechanisms underlying peristaltic reflexes evoked by focal electrical nerve stimuli. Results In fecal pellet propulsion study, the velocity of pellet propulsion was significantly faster in the distal colon and decreased gradually to the proximal part of the mid colon. Intraluminal pressure recording studies showed that luminal infusion initiated normal peristaltic contractions (PCs) in 82% trials of the distal colon, compared to that of mid colon. In response to EFS, the incidence of PCs was significantly increased in the distal colon in contrast, the incidence of non-peristaltic contractions (NPCs) was significantly higher in the middle-mid colon, distal-mid colon and distal colon, compared to that of proximal-mid colon. Addition of L-NAME into the bath increased the frequency of NPCs. EFS failed to cause any PCs or NPCs contractions in the presence of hexamethonium, atropine or tetrodotoxin. Conclusions This study has revealed that electrical nerve stimulation of distal colon is the most likely region to elicit a peristaltic wave, com - pared with the mid or proximal colon. Our findings suggest that EFS-evoked PCs can be modulated by endogenous nitric oxide.

    Original languageEnglish
    Pages (from-to)192-203
    Number of pages12
    JournalJournal of Neurogastroenterology and Motility
    Volume19
    Issue number2
    DOIs
    Publication statusPublished - 2013 Apr

    Keywords

    • Anti-peristalsis
    • Colonic transit
    • Nitric oxide

    ASJC Scopus subject areas

    • Clinical Neurology
    • Gastroenterology

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