Abstract
Background/Aims In isolated guinea-pig colon, we investigated regional differences in peristalsis evoked by intrinsic electrical nerve stimulation. Methods Four colonic segments from mid and distal colon of Hartley guinea pigs, were mounted horizontally in an organ bath. Measurement of pellet propulsion time, intraluminal pressure, electrical field stimulation (EFS; 0.5 ms, 60 V, 10 Hz), and response of pharmacological antagonists, were performed to isolated segments of colon to determine the mechanisms underlying peristaltic reflexes evoked by focal electrical nerve stimuli. Results In fecal pellet propulsion study, the velocity of pellet propulsion was significantly faster in the distal colon and decreased gradually to the proximal part of the mid colon. Intraluminal pressure recording studies showed that luminal infusion initiated normal peristaltic contractions (PCs) in 82% trials of the distal colon, compared to that of mid colon. In response to EFS, the incidence of PCs was significantly increased in the distal colon in contrast, the incidence of non-peristaltic contractions (NPCs) was significantly higher in the middle-mid colon, distal-mid colon and distal colon, compared to that of proximal-mid colon. Addition of L-NAME into the bath increased the frequency of NPCs. EFS failed to cause any PCs or NPCs contractions in the presence of hexamethonium, atropine or tetrodotoxin. Conclusions This study has revealed that electrical nerve stimulation of distal colon is the most likely region to elicit a peristaltic wave, com - pared with the mid or proximal colon. Our findings suggest that EFS-evoked PCs can be modulated by endogenous nitric oxide.
| Original language | English |
|---|---|
| Pages (from-to) | 192-203 |
| Number of pages | 12 |
| Journal | Journal of Neurogastroenterology and Motility |
| Volume | 19 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2013 Apr 1 |
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Keywords
- Anti-peristalsis
- Colonic transit
- Nitric oxide
ASJC Scopus subject areas
- Clinical Neurology
- Gastroenterology
Cite this
Regional difference in colonic motility response to electrical field stimulation in guinea pig. / Kwak, Jung-Myun; Babygirija, Reji; Gribovskaja-Rupp, Irena; Takahashi, Toku; Yamato, Shigeru; Ludwig, Kirk.
In: Journal of Neurogastroenterology and Motility, Vol. 19, No. 2, 01.04.2013, p. 192-203.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regional difference in colonic motility response to electrical field stimulation in guinea pig
AU - Kwak, Jung-Myun
AU - Babygirija, Reji
AU - Gribovskaja-Rupp, Irena
AU - Takahashi, Toku
AU - Yamato, Shigeru
AU - Ludwig, Kirk
PY - 2013/4/1
Y1 - 2013/4/1
N2 - Background/Aims In isolated guinea-pig colon, we investigated regional differences in peristalsis evoked by intrinsic electrical nerve stimulation. Methods Four colonic segments from mid and distal colon of Hartley guinea pigs, were mounted horizontally in an organ bath. Measurement of pellet propulsion time, intraluminal pressure, electrical field stimulation (EFS; 0.5 ms, 60 V, 10 Hz), and response of pharmacological antagonists, were performed to isolated segments of colon to determine the mechanisms underlying peristaltic reflexes evoked by focal electrical nerve stimuli. Results In fecal pellet propulsion study, the velocity of pellet propulsion was significantly faster in the distal colon and decreased gradually to the proximal part of the mid colon. Intraluminal pressure recording studies showed that luminal infusion initiated normal peristaltic contractions (PCs) in 82% trials of the distal colon, compared to that of mid colon. In response to EFS, the incidence of PCs was significantly increased in the distal colon in contrast, the incidence of non-peristaltic contractions (NPCs) was significantly higher in the middle-mid colon, distal-mid colon and distal colon, compared to that of proximal-mid colon. Addition of L-NAME into the bath increased the frequency of NPCs. EFS failed to cause any PCs or NPCs contractions in the presence of hexamethonium, atropine or tetrodotoxin. Conclusions This study has revealed that electrical nerve stimulation of distal colon is the most likely region to elicit a peristaltic wave, com - pared with the mid or proximal colon. Our findings suggest that EFS-evoked PCs can be modulated by endogenous nitric oxide.
AB - Background/Aims In isolated guinea-pig colon, we investigated regional differences in peristalsis evoked by intrinsic electrical nerve stimulation. Methods Four colonic segments from mid and distal colon of Hartley guinea pigs, were mounted horizontally in an organ bath. Measurement of pellet propulsion time, intraluminal pressure, electrical field stimulation (EFS; 0.5 ms, 60 V, 10 Hz), and response of pharmacological antagonists, were performed to isolated segments of colon to determine the mechanisms underlying peristaltic reflexes evoked by focal electrical nerve stimuli. Results In fecal pellet propulsion study, the velocity of pellet propulsion was significantly faster in the distal colon and decreased gradually to the proximal part of the mid colon. Intraluminal pressure recording studies showed that luminal infusion initiated normal peristaltic contractions (PCs) in 82% trials of the distal colon, compared to that of mid colon. In response to EFS, the incidence of PCs was significantly increased in the distal colon in contrast, the incidence of non-peristaltic contractions (NPCs) was significantly higher in the middle-mid colon, distal-mid colon and distal colon, compared to that of proximal-mid colon. Addition of L-NAME into the bath increased the frequency of NPCs. EFS failed to cause any PCs or NPCs contractions in the presence of hexamethonium, atropine or tetrodotoxin. Conclusions This study has revealed that electrical nerve stimulation of distal colon is the most likely region to elicit a peristaltic wave, com - pared with the mid or proximal colon. Our findings suggest that EFS-evoked PCs can be modulated by endogenous nitric oxide.
KW - Anti-peristalsis
KW - Colonic transit
KW - Nitric oxide
UR - http://www.scopus.com/inward/record.url?scp=84876699219&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876699219&partnerID=8YFLogxK
U2 - 10.5056/jnm.2013.19.2.192
DO - 10.5056/jnm.2013.19.2.192
M3 - Article
C2 - 23667750
AN - SCOPUS:84876699219
VL - 19
SP - 192
EP - 203
JO - Journal of Neurogastroenterology and Motility
JF - Journal of Neurogastroenterology and Motility
SN - 2093-0879
IS - 2
ER -