Abstract
σB, a homolog of stress-responsive σB of Bacillus subtilis, controls both osmoprotection and differentiation in Streptomyces coelicolor A3 (2). Its gene is preceded by rsbA and rsbB genes encoding homologs of an anti-sigma factor, RsbW, and its antagonist, RsbV, of B. subtilis, respectively. Purified RsbA bound to σB and prevented σB-directed transcription from the sigBp1 promoter in vitro. An rsbA-null mutant exhibited contrasting behavior to the sigB mutant, with elevated sigBp1 transcription, no actinorhodin production, and precocious aerial mycelial formation, reflecting enhanced activity of σB in vivo. Despite sequence similarity to RsbV, RsbB lacks the conserved phosphorylatable serine residue and its gene disruption produced no distinct phenotype. RsbV (SCO7325) from a putative six-gene operon (rsbV-rsbR-rsbS-rsbT- rsbU1-rsbU) was strongly induced by osmotic stress in a σB- dependent manner. It antagonized the inhibitory action of RsbA on σB-directed transcription and was phosphorylated by RsbA in vitro. These results support the hypothesis that the rapid induction of σB target genes by osmotic stress results from modulation of σB activity by the kinase-anti-sigma factor RsbA and its phosphorylatable antagonist RsbV, which function by a partner-switching mechanism. Amplified induction could result from a rapid increase in the synthesis of both σB and its inhibitor antagonist.
| Original language | English |
|---|---|
| Pages (from-to) | 8490-8498 |
| Number of pages | 9 |
| Journal | Journal of Bacteriology |
| Volume | 186 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - 2004 Dec 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Molecular Biology
Cite this
Regulation of σB by an anti- and an anti-anti-sigma factor in Streptomyces coelicolor in response to osmotic stress. / Lee, Eun-Jin; Cho, You Hee; Kim, Hyo Sub; Ahn, Bo Eun; Roe, Jung Hye.
In: Journal of Bacteriology, Vol. 186, No. 24, 01.12.2004, p. 8490-8498.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Regulation of σB by an anti- and an anti-anti-sigma factor in Streptomyces coelicolor in response to osmotic stress
AU - Lee, Eun-Jin
AU - Cho, You Hee
AU - Kim, Hyo Sub
AU - Ahn, Bo Eun
AU - Roe, Jung Hye
PY - 2004/12/1
Y1 - 2004/12/1
N2 - σB, a homolog of stress-responsive σB of Bacillus subtilis, controls both osmoprotection and differentiation in Streptomyces coelicolor A3 (2). Its gene is preceded by rsbA and rsbB genes encoding homologs of an anti-sigma factor, RsbW, and its antagonist, RsbV, of B. subtilis, respectively. Purified RsbA bound to σB and prevented σB-directed transcription from the sigBp1 promoter in vitro. An rsbA-null mutant exhibited contrasting behavior to the sigB mutant, with elevated sigBp1 transcription, no actinorhodin production, and precocious aerial mycelial formation, reflecting enhanced activity of σB in vivo. Despite sequence similarity to RsbV, RsbB lacks the conserved phosphorylatable serine residue and its gene disruption produced no distinct phenotype. RsbV (SCO7325) from a putative six-gene operon (rsbV-rsbR-rsbS-rsbT- rsbU1-rsbU) was strongly induced by osmotic stress in a σB- dependent manner. It antagonized the inhibitory action of RsbA on σB-directed transcription and was phosphorylated by RsbA in vitro. These results support the hypothesis that the rapid induction of σB target genes by osmotic stress results from modulation of σB activity by the kinase-anti-sigma factor RsbA and its phosphorylatable antagonist RsbV, which function by a partner-switching mechanism. Amplified induction could result from a rapid increase in the synthesis of both σB and its inhibitor antagonist.
AB - σB, a homolog of stress-responsive σB of Bacillus subtilis, controls both osmoprotection and differentiation in Streptomyces coelicolor A3 (2). Its gene is preceded by rsbA and rsbB genes encoding homologs of an anti-sigma factor, RsbW, and its antagonist, RsbV, of B. subtilis, respectively. Purified RsbA bound to σB and prevented σB-directed transcription from the sigBp1 promoter in vitro. An rsbA-null mutant exhibited contrasting behavior to the sigB mutant, with elevated sigBp1 transcription, no actinorhodin production, and precocious aerial mycelial formation, reflecting enhanced activity of σB in vivo. Despite sequence similarity to RsbV, RsbB lacks the conserved phosphorylatable serine residue and its gene disruption produced no distinct phenotype. RsbV (SCO7325) from a putative six-gene operon (rsbV-rsbR-rsbS-rsbT- rsbU1-rsbU) was strongly induced by osmotic stress in a σB- dependent manner. It antagonized the inhibitory action of RsbA on σB-directed transcription and was phosphorylated by RsbA in vitro. These results support the hypothesis that the rapid induction of σB target genes by osmotic stress results from modulation of σB activity by the kinase-anti-sigma factor RsbA and its phosphorylatable antagonist RsbV, which function by a partner-switching mechanism. Amplified induction could result from a rapid increase in the synthesis of both σB and its inhibitor antagonist.
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UR - http://www.scopus.com/inward/citedby.url?scp=10044280434&partnerID=8YFLogxK
U2 - 10.1128/JB.186.24.8490-8498.2004
DO - 10.1128/JB.186.24.8490-8498.2004
M3 - Article
C2 - 15576799
AN - SCOPUS:10044280434
VL - 186
SP - 8490
EP - 8498
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 24
ER -