Regulation of ADP-ribosylation factor 4 expression by small leucine zipper protein and involvement in breast cancer cell migration

Soon Young Jang, Sung Wuk Jang, Je Sang Ko

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

ADP-ribosylation factor 4 (ARF4) is a member of the Ras superfamily of small guanine nucleotide-binding proteins. ARF4 is known to interact with the epidermal growth factor receptor (EGFR) and mediates the EGF-dependent signal pathway, and has an anti-apoptotic function in human glioblastoma-derived U373MG cells. Although ARF4 plays a role in cancer cells, the molecular mechanism underlying regulation of its expression and its exact functions in breast cancer are unknown. In this study, we investigated the regulatory mechanism of ARF4 expression and its involvement in breast cancer cell migration. Our results show that phorbol 12-myristate 13-acetate (PMA) treatment increases ARF4 expression at both the transcriptional and translational levels. We found that the novel transcription factor small leucine zipper protein (sLZIP) binds directly to the CRE motif of the -43 to -35 region in the ARF4 promoter and regulates PMA-induced ARF4 expression. We also found that PMA-stimulated ARF4 expression increases AP-1 promoter activity, leading to induction of breast cancer cell migration. These results indicate that sLZIP-regulated ARF4 expression in response to PMA is involved in breast cancer cell migration, and sLZIP and ARF4 are potential therapeutic target molecules for treating breast cancer invasion and metastasis.

Original languageEnglish
Pages (from-to)185-197
Number of pages13
JournalCancer Letters
Volume314
Issue number2
DOIs
Publication statusPublished - 2012 Jan 28

Fingerprint

ADP-Ribosylation Factors
Leucine Zippers
Cell Movement
Cells
Breast Neoplasms
Proteins
Acetates
Guanine Nucleotides
Transcription Factor AP-1
Glioblastoma
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Signal Transduction
Carrier Proteins
Transcription Factors

Keywords

  • ARF4
  • Breast cancer
  • Cell migration
  • slZIP

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Regulation of ADP-ribosylation factor 4 expression by small leucine zipper protein and involvement in breast cancer cell migration. / Jang, Soon Young; Jang, Sung Wuk; Ko, Je Sang.

In: Cancer Letters, Vol. 314, No. 2, 28.01.2012, p. 185-197.

Research output: Contribution to journalArticle

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