Regulation of fibronectin gene expression by cyclic AMP and phorbol myristate acetate in HT-1080 human fibrosarcoma cells

Byung Heon Lee, Rang Woon Park, In San Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We studied the regulation of fibronectin (FN) gene expression by cAMP and phorbol-12-myristate-13-acetate (PMA) in HT-1080 human fibrosarcoma cells. Dibutyryl cAMP increased FN synthesis and mRNA levels, while PMA inhibited the cAMP-induced FN synthesis. In transient transfection assays, cAMP increased FN promoter activity, while PMA paradoxically enhanced the cAMP-induced promoter activity. Stable transfection experiments, however, showed that neither cAMP or PMA alone nor together affected FN promoter activity. These results suggest that PMA antagonizes the cAMP-induced FN gene expression and that both the action of cAMP and the inhibition of its action by PMA may occur at the posttranscriptional level in HT-1080 cells.

Original languageEnglish
Pages (from-to)240-245
Number of pages6
JournalExperimental and Molecular Medicine
Volume30
Issue number4
DOIs
Publication statusPublished - 1998 Dec 31

Keywords

  • Fibronectin
  • HT-1080
  • Phorbol myristate acetate
  • Protein kinase C
  • cAMP

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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