Regulation of human LZIP expression by NF-κB and its involvement in monocyte cell migration induced by Lkn-1

Sung Wuk Jang, Suk Kim Yoon, Rim Kim Yoon, Joong Sung Ho, Je Sang Ko

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Human LZIP is a transcription factor that is involved in leukocyte cell mobility. Expression of LZIP is known to differentially regulate monocyte cell migration induced by CCR1-dependent chemokines. However, its transcriptional regulation has not been characterized. Our results indicate that Lkn-1 induces LZIP expression in a time- and dose-dependent manner, and the induction of LZIP shows an immediate early response to Lkn-1. We identified and cloned ∼1.4 kb of the LZIP promoter from a human genomic DNA. To identify regulatory elements controlling restricted expression of LZIP, deletion mutants were constructed from the 1469-bp LZIP promoter region (-1219/+251) linked to the luciferase reporter gene. Maximal promoter activity was contained within 613 bp from the tentative transcription initiation site and was sharply reduced in a truncated construct (-338+251). This promoter sequence contained consensus NF-κB- and Sp-1-binding sites. Results from an inhibitor assay showed that NF-κB is involved in Lkn-1-induced LZIP expression, but Sp-1 is not. We also demonstrated that NF-κB binds to the LZIP promoter and that the binding is specific, as revealed by an electrophoretic mobility shift assay and a mutation analysis. Chemotaxis analysis showed that LZIP expression because of the NF-κB subfamily is specifically involved in Lkn-1-induced chemotaxis. Our findings suggest that transcription factor NF-κB plays an important role in regulation of LZIP expression, and LZIP expression regulates the monocyte cell migration induced by Lkn-1.

Original languageEnglish
Pages (from-to)11092-11100
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number15
DOIs
Publication statusPublished - 2007 Apr 13

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Chemotaxis
Cell Movement
Monocytes
Assays
Transcription Factors
Electrophoretic mobility
Transcription Initiation Site
Consensus Sequence
Electrophoretic Mobility Shift Assay
Luciferases
Reporter Genes
Chemokines
Genetic Promoter Regions
Leukocytes
Genes
Binding Sites
Mutation
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation of human LZIP expression by NF-κB and its involvement in monocyte cell migration induced by Lkn-1. / Jang, Sung Wuk; Yoon, Suk Kim; Yoon, Rim Kim; Ho, Joong Sung; Ko, Je Sang.

In: Journal of Biological Chemistry, Vol. 282, No. 15, 13.04.2007, p. 11092-11100.

Research output: Contribution to journalArticle

Jang, Sung Wuk ; Yoon, Suk Kim ; Yoon, Rim Kim ; Ho, Joong Sung ; Ko, Je Sang. / Regulation of human LZIP expression by NF-κB and its involvement in monocyte cell migration induced by Lkn-1. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 15. pp. 11092-11100.
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