Regulation of secondary antigen-specific CD8+ T-cell responses by natural killer T cells

Changwan Hong, Hyunji Lee, Yoon Kyung Park, Junghoon Shin, Sundo Jung, Hoyeon Kim, Seokmann Hong, Se-Ho Park

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The physiologic function of natural killer T (NKT) cells in adaptive immunity remains largely unknown because most studies have used NKT cell agonists. In the present study, the role of NKT cells during the secondary effector phase was investigated separately from the primary immunization phase via adoptive transfer of differentiated effector T cells into naive recipients. We found that secondary antitumor CD8+ T-cell responses were optimal when NKT cells were present. Tumor-specific CD8+ effector T cells responded less strongly to tumor cell challenge in NKT cell-deficient recipients than in recipients with intact NKT cells. NKT cell-mediated enhancement of the secondary antitumor CD8+ T-cell response was concurrent with increased number and activity of tumorspecific CD8+ T cells. These findings provide the first demonstration of a direct role for NKT cells in the regulation of antigen-specific secondary T-cell responses without the use of exogenous NKT cell agonists such as α-galactosylceramide (α-GalCer). Furthermore, forced activation of NKT cells with α-GalCer during the secondary immune response in suboptimally immunized animals enhanced otherwise poor tumor rejection responses. Taken together, our findings strongly emphasize the importance of NKT cells in secondary CD8+ T-cell immune responses.

Original languageEnglish
Pages (from-to)4301-4308
Number of pages8
JournalCancer Research
Volume69
Issue number10
DOIs
Publication statusPublished - 2009 May 15

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CD8 Antigens
Natural Killer T-Cells
T-Lymphocytes
Galactosylceramides
Neoplasms
Adoptive Transfer
Adaptive Immunity
Immunization

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Regulation of secondary antigen-specific CD8+ T-cell responses by natural killer T cells. / Hong, Changwan; Lee, Hyunji; Park, Yoon Kyung; Shin, Junghoon; Jung, Sundo; Kim, Hoyeon; Hong, Seokmann; Park, Se-Ho.

In: Cancer Research, Vol. 69, No. 10, 15.05.2009, p. 4301-4308.

Research output: Contribution to journalArticle

Hong, Changwan ; Lee, Hyunji ; Park, Yoon Kyung ; Shin, Junghoon ; Jung, Sundo ; Kim, Hoyeon ; Hong, Seokmann ; Park, Se-Ho. / Regulation of secondary antigen-specific CD8+ T-cell responses by natural killer T cells. In: Cancer Research. 2009 ; Vol. 69, No. 10. pp. 4301-4308.
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