Regulatory functions of SnRK1 in stress-responsive gene expression and in plant growth and development

Young Hee Cho, Jung Woo Hong, Eun Chul Kim, Sang-Dong Yoo

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Sucrose-nonfermentation1-related protein kinase1 (SnRK1) is an evolutionarily conserved energy sensor protein that regulates gene expression in response to energy depletion in plants. Efforts to elucidate the functions and mechanisms of this protein kinase are hampered, however, by inherent growth defects of snrk1-null mutant plants. To overcome these limitations and study SnRK1 functions in vivo, we applied a method combining transient expression in leaf mesophyll protoplasts and stable expression in transgenic plants. We found that both rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana) SnRK1 activities critically influence stress-inducible gene expression and the induction of stress tolerance. Genetic, molecular, and chromatin immunoprecipitation analyses further revealed that the nuclear SnRK1 modulated target gene transcription in a submergencedependent manner. From early seedling development through late senescence, SnRK1 activities appeared to modulate developmental processes in the plants. Our findings offer insight into the regulatory functions of plant SnRK1 in stressresponsive gene regulation and in plant growth and development throughout the life cycle.

Original languageEnglish
Pages (from-to)1955-1964
Number of pages10
JournalPlant Physiology
Volume158
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1
Externally publishedYes

Fingerprint

Plant Development
Growth and Development
Sucrose
plant development
growth and development
plant growth
sucrose
Gene Expression
gene expression
Proteins
proteins
Arabidopsis
Protoplasts
Chromatin Immunoprecipitation
Genetically Modified Plants
energy
Life Cycle Stages
Seedlings
molecular genetics
protein kinases

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Regulatory functions of SnRK1 in stress-responsive gene expression and in plant growth and development. / Cho, Young Hee; Hong, Jung Woo; Kim, Eun Chul; Yoo, Sang-Dong.

In: Plant Physiology, Vol. 158, No. 4, 01.04.2012, p. 1955-1964.

Research output: Contribution to journalArticle

Cho, Young Hee ; Hong, Jung Woo ; Kim, Eun Chul ; Yoo, Sang-Dong. / Regulatory functions of SnRK1 in stress-responsive gene expression and in plant growth and development. In: Plant Physiology. 2012 ; Vol. 158, No. 4. pp. 1955-1964.
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