Regulatory mechanisms of IL-2 and IFNγ suppression by quercetin in T helper cells

Eun Sun Yu, Hyun Jung Min, Su Yeon An, Hee Yeon Won, Jeong-Ho Hong, Eun Sook Hwang

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Quercetin is a popular flavonoid compound that is biosynthesized by plants; it is suggested to modulate a variety of inflammatory responses of macrophages and T lymphocytes. Oral administration of quercetin in arthritic rats dramatically diminishes clinical signs of arthritis. Moreover, quercetin ameliorates experimental autoimmune encephalomyelitis, which is associated with Th1-mediated immune responses. Like quercetin inhibits macrophage-induced cytokine production, it also blocks IL-12-dependent JAK-STAT signaling in Th cells. Despite the anti-inflammatory effects of quercetin acting through Th cells, the regulatory mechanisms remain unclear. Here, we studied the function of quercetin in Th cells and found that quercetin suppressed both IFNγ and IL-2 production upon T cell receptor stimulation. Furthermore, we uncovered the regulatory mechanisms of quercetin involved in the inhibition of cytokine production during Th cell activation. The fact that quercetin-derived IFNγ suppression was blocked in T-bet-deficient Th cells demonstrated quercetin act through the modulation of T-bet expression. Whereas IL-2 inhibition by quercetin was independent of T-bet expression, quercetin diminished IL-2Rα expression, which is critical for positive regulatory loop of IL-2 autoactivation. Taken together, quercetin is suggested to repress both IFNγ and IL-2 cytokine production by independent mechanisms; T-bet-dependent IFNγ suppression and IL-2Rα-dependent IL-2 inhibition.

Original languageEnglish
Pages (from-to)70-78
Number of pages9
JournalBiochemical Pharmacology
Volume76
Issue number1
DOIs
Publication statusPublished - 2008 Jul 1

Fingerprint

Quercetin
Helper-Inducer T-Lymphocytes
Interleukin-2
Macrophages
Cytokines
Arthritis
T-cells
Autoimmune Experimental Encephalomyelitis
Interleukin-12
T-Cell Antigen Receptor
Flavonoids
Oral Administration
Rats
Anti-Inflammatory Agents
Chemical activation
Modulation

Keywords

  • IFNγ
  • IL-2
  • Quercetin
  • T-bet
  • Th1

ASJC Scopus subject areas

  • Pharmacology

Cite this

Regulatory mechanisms of IL-2 and IFNγ suppression by quercetin in T helper cells. / Yu, Eun Sun; Min, Hyun Jung; An, Su Yeon; Won, Hee Yeon; Hong, Jeong-Ho; Hwang, Eun Sook.

In: Biochemical Pharmacology, Vol. 76, No. 1, 01.07.2008, p. 70-78.

Research output: Contribution to journalArticle

Yu, Eun Sun ; Min, Hyun Jung ; An, Su Yeon ; Won, Hee Yeon ; Hong, Jeong-Ho ; Hwang, Eun Sook. / Regulatory mechanisms of IL-2 and IFNγ suppression by quercetin in T helper cells. In: Biochemical Pharmacology. 2008 ; Vol. 76, No. 1. pp. 70-78.
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