TY - JOUR
T1 - Regulatory regions 3' of the immunoglobulin heavy chain intronic enhancer differentially affect expression of a heavy chain transgene in resting and activated B cells
AU - Sigurdardottir, D.
AU - Sohn, J.
AU - Kass, J.
AU - Selsing, E.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1995
Y1 - 1995
N2 - We have compared the expression patterns of three Ig heavy chain transgenes. The three constructs differ only by deletion of J-C intron sequences located downstream of the Eμ enhancer region. When stably transfected into a myeloma cell line, all three constructs are expressed at comparable levels. However, transgenic mice carrying each construct show dramatic differences in transgene expression. Our results indicate that, in addition to the Eμ enhancer, at least two regions, RegA and RegS, within the J-C intron influence transgene expression. RegA, located directly downstream of the core Eμ enhancer, is involved in up-regulation of transgene expression after LPS activation of splenocytes. RegS, located within or downstream of the Sμ switch region, is important for normal levels of transgene expression in splenocytes of heavy chain transgenic mice.
AB - We have compared the expression patterns of three Ig heavy chain transgenes. The three constructs differ only by deletion of J-C intron sequences located downstream of the Eμ enhancer region. When stably transfected into a myeloma cell line, all three constructs are expressed at comparable levels. However, transgenic mice carrying each construct show dramatic differences in transgene expression. Our results indicate that, in addition to the Eμ enhancer, at least two regions, RegA and RegS, within the J-C intron influence transgene expression. RegA, located directly downstream of the core Eμ enhancer, is involved in up-regulation of transgene expression after LPS activation of splenocytes. RegS, located within or downstream of the Sμ switch region, is important for normal levels of transgene expression in splenocytes of heavy chain transgenic mice.
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M3 - Article
C2 - 7868895
AN - SCOPUS:0028926450
VL - 154
SP - 2217
EP - 2225
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 5
ER -