Abstract
We have compared the expression patterns of three Ig heavy chain transgenes. The three constructs differ only by deletion of J-C intron sequences located downstream of the Eμ enhancer region. When stably transfected into a myeloma cell line, all three constructs are expressed at comparable levels. However, transgenic mice carrying each construct show dramatic differences in transgene expression. Our results indicate that, in addition to the Eμ enhancer, at least two regions, RegA and RegS, within the J-C intron influence transgene expression. RegA, located directly downstream of the core Eμ enhancer, is involved in up-regulation of transgene expression after LPS activation of splenocytes. RegS, located within or downstream of the Sμ switch region, is important for normal levels of transgene expression in splenocytes of heavy chain transgenic mice.
| Original language | English |
|---|---|
| Pages (from-to) | 2217-2225 |
| Number of pages | 9 |
| Journal | Journal of Immunology |
| Volume | 154 |
| Issue number | 5 |
| Publication status | Published - 1995 Jan 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Regulatory regions 3' of the immunoglobulin heavy chain intronic enhancer differentially affect expression of a heavy chain transgene in resting and activated B cells. / Sigurdardottir, D.; Sohn, Jeongwon; Kass, J.; Selsing, E.
In: Journal of Immunology, Vol. 154, No. 5, 01.01.1995, p. 2217-2225.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulatory regions 3' of the immunoglobulin heavy chain intronic enhancer differentially affect expression of a heavy chain transgene in resting and activated B cells
AU - Sigurdardottir, D.
AU - Sohn, Jeongwon
AU - Kass, J.
AU - Selsing, E.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - We have compared the expression patterns of three Ig heavy chain transgenes. The three constructs differ only by deletion of J-C intron sequences located downstream of the Eμ enhancer region. When stably transfected into a myeloma cell line, all three constructs are expressed at comparable levels. However, transgenic mice carrying each construct show dramatic differences in transgene expression. Our results indicate that, in addition to the Eμ enhancer, at least two regions, RegA and RegS, within the J-C intron influence transgene expression. RegA, located directly downstream of the core Eμ enhancer, is involved in up-regulation of transgene expression after LPS activation of splenocytes. RegS, located within or downstream of the Sμ switch region, is important for normal levels of transgene expression in splenocytes of heavy chain transgenic mice.
AB - We have compared the expression patterns of three Ig heavy chain transgenes. The three constructs differ only by deletion of J-C intron sequences located downstream of the Eμ enhancer region. When stably transfected into a myeloma cell line, all three constructs are expressed at comparable levels. However, transgenic mice carrying each construct show dramatic differences in transgene expression. Our results indicate that, in addition to the Eμ enhancer, at least two regions, RegA and RegS, within the J-C intron influence transgene expression. RegA, located directly downstream of the core Eμ enhancer, is involved in up-regulation of transgene expression after LPS activation of splenocytes. RegS, located within or downstream of the Sμ switch region, is important for normal levels of transgene expression in splenocytes of heavy chain transgenic mice.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028926450&partnerID=8YFLogxK
M3 - Article
VL - 154
SP - 2217
EP - 2225
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 5
ER -