Regulatory regions 3' of the immunoglobulin heavy chain intronic enhancer differentially affect expression of a heavy chain transgene in resting and activated B cells

D. Sigurdardottir, Jeongwon Sohn, J. Kass, E. Selsing

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have compared the expression patterns of three Ig heavy chain transgenes. The three constructs differ only by deletion of J-C intron sequences located downstream of the Eμ enhancer region. When stably transfected into a myeloma cell line, all three constructs are expressed at comparable levels. However, transgenic mice carrying each construct show dramatic differences in transgene expression. Our results indicate that, in addition to the Eμ enhancer, at least two regions, RegA and RegS, within the J-C intron influence transgene expression. RegA, located directly downstream of the core Eμ enhancer, is involved in up-regulation of transgene expression after LPS activation of splenocytes. RegS, located within or downstream of the Sμ switch region, is important for normal levels of transgene expression in splenocytes of heavy chain transgenic mice.

Original languageEnglish
Pages (from-to)2217-2225
Number of pages9
JournalJournal of Immunology
Volume154
Issue number5
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Fingerprint

Immunoglobulin Heavy Chains
Nucleic Acid Regulatory Sequences
Transgenes
B-Lymphocytes
Introns
Transgenic Mice
Up-Regulation
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Regulatory regions 3' of the immunoglobulin heavy chain intronic enhancer differentially affect expression of a heavy chain transgene in resting and activated B cells. / Sigurdardottir, D.; Sohn, Jeongwon; Kass, J.; Selsing, E.

In: Journal of Immunology, Vol. 154, No. 5, 01.01.1995, p. 2217-2225.

Research output: Contribution to journalArticle

@article{4b470a501a0347078cfd21bbd82bd857,
title = "Regulatory regions 3' of the immunoglobulin heavy chain intronic enhancer differentially affect expression of a heavy chain transgene in resting and activated B cells",
abstract = "We have compared the expression patterns of three Ig heavy chain transgenes. The three constructs differ only by deletion of J-C intron sequences located downstream of the Eμ enhancer region. When stably transfected into a myeloma cell line, all three constructs are expressed at comparable levels. However, transgenic mice carrying each construct show dramatic differences in transgene expression. Our results indicate that, in addition to the Eμ enhancer, at least two regions, RegA and RegS, within the J-C intron influence transgene expression. RegA, located directly downstream of the core Eμ enhancer, is involved in up-regulation of transgene expression after LPS activation of splenocytes. RegS, located within or downstream of the Sμ switch region, is important for normal levels of transgene expression in splenocytes of heavy chain transgenic mice.",
author = "D. Sigurdardottir and Jeongwon Sohn and J. Kass and E. Selsing",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "154",
pages = "2217--2225",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

TY - JOUR

T1 - Regulatory regions 3' of the immunoglobulin heavy chain intronic enhancer differentially affect expression of a heavy chain transgene in resting and activated B cells

AU - Sigurdardottir, D.

AU - Sohn, Jeongwon

AU - Kass, J.

AU - Selsing, E.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - We have compared the expression patterns of three Ig heavy chain transgenes. The three constructs differ only by deletion of J-C intron sequences located downstream of the Eμ enhancer region. When stably transfected into a myeloma cell line, all three constructs are expressed at comparable levels. However, transgenic mice carrying each construct show dramatic differences in transgene expression. Our results indicate that, in addition to the Eμ enhancer, at least two regions, RegA and RegS, within the J-C intron influence transgene expression. RegA, located directly downstream of the core Eμ enhancer, is involved in up-regulation of transgene expression after LPS activation of splenocytes. RegS, located within or downstream of the Sμ switch region, is important for normal levels of transgene expression in splenocytes of heavy chain transgenic mice.

AB - We have compared the expression patterns of three Ig heavy chain transgenes. The three constructs differ only by deletion of J-C intron sequences located downstream of the Eμ enhancer region. When stably transfected into a myeloma cell line, all three constructs are expressed at comparable levels. However, transgenic mice carrying each construct show dramatic differences in transgene expression. Our results indicate that, in addition to the Eμ enhancer, at least two regions, RegA and RegS, within the J-C intron influence transgene expression. RegA, located directly downstream of the core Eμ enhancer, is involved in up-regulation of transgene expression after LPS activation of splenocytes. RegS, located within or downstream of the Sμ switch region, is important for normal levels of transgene expression in splenocytes of heavy chain transgenic mice.

UR - http://www.scopus.com/inward/record.url?scp=0028926450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028926450&partnerID=8YFLogxK

M3 - Article

VL - 154

SP - 2217

EP - 2225

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -