Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps

Pierre Regis Burgel, Estelle Escudier, Andre Coste, Trang Dao-Pick, Iris F. Ueki, Kiyoshi Takeyama, Jae Jeong Shim, Andrew H. Murr, Jay A. Nadel

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Background: Because the epidermal growth factor receptor (EGFR) system regulates mucin production in airway epithelium, we hypothesized a role for this system in mucus hypersecretion that occurs in nasal polyposis. Objective: We examined the relationship between goblet cell hyperplasia, EGFR expression, and inflammatory mediators produced by eosinophils and neutrophils in nasal polyp tissues. Methods: Nasal polyp tissue samples from 8 patients and nasal turbinate biopsy specimens from 6 normal control subjects were examined for alcian blue/PAS staining, mucin MUC5AC (MUC5AC), and EGFR immunoreactivity and EGFR gene expression (in situ hybridization). We also examined the role of eosinophils and neutrophils in goblet cell hyperplasia. Results: In control nasal mucosa alcian blue/periodic acid-Schiff- and MUC5AC-stained areas were 18.40% ± 1.31% and 21.89% ± 1.43%, respectively. In polyps the alcian blue/periodic acid-Schiff- and MUC5AC-stained areas were 51.30% ± 5.85% and 52.07% ± 6.58%, which was significantly larger than that found in control subjects (each comparison, P < .01). Four of 6 control specimens expressed EGFR messenger RNA and protein weakly in the epithelium. In polyps 4 of 8 specimens expressed EGFR gene and EGFR protein strongly; the EGFR-stained area was greater in hyperplastic than in pseudostratified epithelium. TNF-α immunoreactivity, expressed in eosinophils, was increased in EGFR-positive polyps compared with EGFR-negative polyps, suggesting a role for TNF-α in EGFR expression. Neutrophils were increased in the epithelium of EGFR-positive compared with EGFR-negative polyps, suggesting a role for these cells in mucin expression and in goblet cell degranulation. Conclusion: These data suggest a role for EGFR cascade in the regulation of goblet cell mucins in nasal polyps. Proof of concept will require clinical studies using selective EGFR inhibitors.

Original languageEnglish
Pages (from-to)705-712
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume106
Issue number4
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Fingerprint

Nasal Polyps
Goblet Cells
Epidermal Growth Factor Receptor
Hyperplasia
Polyps
Mucins
Alcian Blue
Epithelium
Eosinophils
erbB-1 Genes
Periodic Acid
Neutrophils
Nose
Cell Degranulation
Turbinates
Nasal Mucosa
Mucus
In Situ Hybridization

Keywords

  • Airway epithelium
  • Eosinophils
  • Epidermal growth factor receptor
  • Mucin MUC5AC
  • Nasal polyps
  • Neutrophils

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Burgel, P. R., Escudier, E., Coste, A., Dao-Pick, T., Ueki, I. F., Takeyama, K., ... Nadel, J. A. (2000). Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps. Journal of Allergy and Clinical Immunology, 106(4), 705-712.

Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps. / Burgel, Pierre Regis; Escudier, Estelle; Coste, Andre; Dao-Pick, Trang; Ueki, Iris F.; Takeyama, Kiyoshi; Shim, Jae Jeong; Murr, Andrew H.; Nadel, Jay A.

In: Journal of Allergy and Clinical Immunology, Vol. 106, No. 4, 01.01.2000, p. 705-712.

Research output: Contribution to journalArticle

Burgel, PR, Escudier, E, Coste, A, Dao-Pick, T, Ueki, IF, Takeyama, K, Shim, JJ, Murr, AH & Nadel, JA 2000, 'Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps', Journal of Allergy and Clinical Immunology, vol. 106, no. 4, pp. 705-712.
Burgel PR, Escudier E, Coste A, Dao-Pick T, Ueki IF, Takeyama K et al. Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps. Journal of Allergy and Clinical Immunology. 2000 Jan 1;106(4):705-712.
Burgel, Pierre Regis ; Escudier, Estelle ; Coste, Andre ; Dao-Pick, Trang ; Ueki, Iris F. ; Takeyama, Kiyoshi ; Shim, Jae Jeong ; Murr, Andrew H. ; Nadel, Jay A. / Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps. In: Journal of Allergy and Clinical Immunology. 2000 ; Vol. 106, No. 4. pp. 705-712.
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abstract = "Background: Because the epidermal growth factor receptor (EGFR) system regulates mucin production in airway epithelium, we hypothesized a role for this system in mucus hypersecretion that occurs in nasal polyposis. Objective: We examined the relationship between goblet cell hyperplasia, EGFR expression, and inflammatory mediators produced by eosinophils and neutrophils in nasal polyp tissues. Methods: Nasal polyp tissue samples from 8 patients and nasal turbinate biopsy specimens from 6 normal control subjects were examined for alcian blue/PAS staining, mucin MUC5AC (MUC5AC), and EGFR immunoreactivity and EGFR gene expression (in situ hybridization). We also examined the role of eosinophils and neutrophils in goblet cell hyperplasia. Results: In control nasal mucosa alcian blue/periodic acid-Schiff- and MUC5AC-stained areas were 18.40{\%} ± 1.31{\%} and 21.89{\%} ± 1.43{\%}, respectively. In polyps the alcian blue/periodic acid-Schiff- and MUC5AC-stained areas were 51.30{\%} ± 5.85{\%} and 52.07{\%} ± 6.58{\%}, which was significantly larger than that found in control subjects (each comparison, P < .01). Four of 6 control specimens expressed EGFR messenger RNA and protein weakly in the epithelium. In polyps 4 of 8 specimens expressed EGFR gene and EGFR protein strongly; the EGFR-stained area was greater in hyperplastic than in pseudostratified epithelium. TNF-α immunoreactivity, expressed in eosinophils, was increased in EGFR-positive polyps compared with EGFR-negative polyps, suggesting a role for TNF-α in EGFR expression. Neutrophils were increased in the epithelium of EGFR-positive compared with EGFR-negative polyps, suggesting a role for these cells in mucin expression and in goblet cell degranulation. Conclusion: These data suggest a role for EGFR cascade in the regulation of goblet cell mucins in nasal polyps. Proof of concept will require clinical studies using selective EGFR inhibitors.",
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AU - Ueki, Iris F.

AU - Takeyama, Kiyoshi

AU - Shim, Jae Jeong

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