Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps

Pierre Regis Burgel, Estelle Escudier, Andre Coste, Trang Dao-Pick, Iris F. Ueki, Kiyoshi Takeyama, Jae Jeong Shim, Andrew H. Murr, Jay A. Nadel

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Background: Because the epidermal growth factor receptor (EGFR) system regulates mucin production in airway epithelium, we hypothesized a role for this system in mucus hypersecretion that occurs in nasal polyposis. Objective: We examined the relationship between goblet cell hyperplasia, EGFR expression, and inflammatory mediators produced by eosinophils and neutrophils in nasal polyp tissues. Methods: Nasal polyp tissue samples from 8 patients and nasal turbinate biopsy specimens from 6 normal control subjects were examined for alcian blue/PAS staining, mucin MUC5AC (MUC5AC), and EGFR immunoreactivity and EGFR gene expression (in situ hybridization). We also examined the role of eosinophils and neutrophils in goblet cell hyperplasia. Results: In control nasal mucosa alcian blue/periodic acid-Schiff- and MUC5AC-stained areas were 18.40% ± 1.31% and 21.89% ± 1.43%, respectively. In polyps the alcian blue/periodic acid-Schiff- and MUC5AC-stained areas were 51.30% ± 5.85% and 52.07% ± 6.58%, which was significantly larger than that found in control subjects (each comparison, P < .01). Four of 6 control specimens expressed EGFR messenger RNA and protein weakly in the epithelium. In polyps 4 of 8 specimens expressed EGFR gene and EGFR protein strongly; the EGFR-stained area was greater in hyperplastic than in pseudostratified epithelium. TNF-α immunoreactivity, expressed in eosinophils, was increased in EGFR-positive polyps compared with EGFR-negative polyps, suggesting a role for TNF-α in EGFR expression. Neutrophils were increased in the epithelium of EGFR-positive compared with EGFR-negative polyps, suggesting a role for these cells in mucin expression and in goblet cell degranulation. Conclusion: These data suggest a role for EGFR cascade in the regulation of goblet cell mucins in nasal polyps. Proof of concept will require clinical studies using selective EGFR inhibitors.

Original languageEnglish
Pages (from-to)705-712
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume106
Issue number4
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Airway epithelium
  • Eosinophils
  • Epidermal growth factor receptor
  • Mucin MUC5AC
  • Nasal polyps
  • Neutrophils

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps'. Together they form a unique fingerprint.

Cite this