Relationship between agitation speed and the morphological characteristics of Verticillium lecanii CS-625 during spore production

Jin Young Lee, Seong Woo Kang, Seung Wook Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Verticillium lecanii is recognized as an entomopathogenic fungus, and has high potential in the biological control of pests. In this study, it was investigated that the relationship between agitation speed in a 2.5 L stirred tank reactor (STR) at 25°C and initial pH 5.5, and the morphological characteristics of V. lecanii CS-625, such as hyphal length/width, spore length/width, and the number of tips during spore production. The agitation speed affected the hyphae patterns and the number of tips. The number of spores rapidly increased at 48 to 60 h of cultivation, and the highest spore productivity (2.5 × 1010 spore/L·h at 60 h) occurred with an agitation speed of 350 rpm and an aeration rate of 1.0 vvm. The number of tips increased in proportion to the increase in spore production during the same culture time. The highest number of tips (4.8 × 108 tip/mL) was obtained at 72 h of cultivation. The shortest mean spore length (2.8 μm) was obtained at 60 h of cultivation. Therefore, it was determined that the increased number of tips and decreased mean spore length were closely related to the production of V. lecanii spores.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Volume13
Issue number1
DOIs
Publication statusPublished - 2008 Jan

Keywords

  • Entomopathogenic fungi
  • Morphological characteristics
  • Spore
  • Verticillium lecanii

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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