Relationship between dendritic cells and activated eosinophils in induced sputum of asthmatics

Youngil I. Koh, Jee Bum Lee, Se Ryeon Lee, Seung Gyu Ji, Inseon S. Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It has been suggested that dendritic cells (DCs) are critical antigen presenting cells for eosinophilic airway inflammation in a mouse model of asthma, and cysteinyl leukotrienes may play a role in DC trafficking in asthmatics. We investigated whether the number of DCs is increased in the induced sputum of both atopic and nonatopic asthmatics and is related to activated eosinophil count in the sputum. Sputum was induced by inhalation of hypertonic saline in 9 atopic and 12 nonatopic asthmatics and 10 nonatopic normal controls, and differential cell counts were performed. DCs and activated eosinophils were identified by immunocytochemistry with monoclonal antibodies (anti-CD1a and EG2, respectively). There were significantly higher percentages of eosinophils, EG2+ cells, and CD1a+ DC in the sputum of atopic and nonatopic asthmatics compared with normal controls, respectively. In asthmatics, the percentage of CD1a+ DC was significantly correlated with that of EG2+ cells (Rs=0.62, p=0.004). We demonstrated that the increased number of DCs was evident in the induced sputum of both atopic and nonatopic asthmatics, and the DC number was related to the activated eosinophil count, which suggests that DCs may contribute to the ongoing eosinophilic inflammation in asthmatic airways, and vice versa.

Original languageEnglish
Pages (from-to)384-389
Number of pages6
JournalJournal of Korean Medical Science
Volume20
Issue number3
Publication statusPublished - 2005 Jun 1
Externally publishedYes

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Sputum
Eosinophils
Dendritic Cells
Cell Count
Inflammation
Antigen-Presenting Cells
Inhalation
Asthma
Immunohistochemistry
Monoclonal Antibodies

Keywords

  • Asthma
  • Dendritic Cells
  • Eosinophils

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Relationship between dendritic cells and activated eosinophils in induced sputum of asthmatics. / Koh, Youngil I.; Lee, Jee Bum; Lee, Se Ryeon; Ji, Seung Gyu; Choi, Inseon S.

In: Journal of Korean Medical Science, Vol. 20, No. 3, 01.06.2005, p. 384-389.

Research output: Contribution to journalArticle

Koh, Youngil I. ; Lee, Jee Bum ; Lee, Se Ryeon ; Ji, Seung Gyu ; Choi, Inseon S. / Relationship between dendritic cells and activated eosinophils in induced sputum of asthmatics. In: Journal of Korean Medical Science. 2005 ; Vol. 20, No. 3. pp. 384-389.
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