Abstract
Objective To investigate whether the uterine cervix excision is associated with preterm birth in female mice. Study Design Sexually mature female C57BL/6 mice ( n = 40) were randomly divided into four groups (A, sham; B, cervical excision; C, lipopolysaccharide, 100 µg intrauterine injection; and D, cervical excision + lipopolysaccharide injection), with 10 mice per group. Three weeks after cervical excision, timed mating was performed. On gestational day 16, lipopolysaccharide was injected between the first and second horns of the right uterus near the cervix. The uterine cervix was obtained after delivery and was histologically analyzed. Results The mean gestational period in group D was significantly lower than those in the other groups (17, 19.5, 19, and 18.2 days in groups D, A, B, and C, respectively; p = 0.034). The cervical length was shorter in the cervical excision groups ( p = 0.004). The muscle-to-collagen ratio in the proximal cervix was higher in group D ( p = 0.037). Conclusion Prepregnancy cervical excision and subsequent lipopolysaccharide injection showed a high rate of preterm birth, which was higher than the known lipopolysaccharide injection related preterm birth rate. Prepregnancy cervical excision appears to have additive effects with inflammation in inducing preterm birth, which are associated with the relative muscular component amount.
| Original language | English |
|---|---|
| Journal | American Journal of Perinatology |
| DOIs | |
| Publication status | Accepted/In press - 2017 May 9 |
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Keywords
- excision
- infection
- mouse model
- preterm birth
- uterine cervix
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
- Obstetrics and Gynaecology
Cite this
Relationship between Partial Uterine Cervical Tissue Excision and Preterm Birth : An Experimental Animal Study. / Ahn, Ki Hoon; Jeong, Hyun Chul; Kim, Hee youn; Kang, Dahyun; Hong, Soon Cheol; Cho, Geum-Joon; Oh, Min Jeong; Kim, Hai Joong.
In: American Journal of Perinatology, 09.05.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relationship between Partial Uterine Cervical Tissue Excision and Preterm Birth
T2 - An Experimental Animal Study
AU - Ahn, Ki Hoon
AU - Jeong, Hyun Chul
AU - Kim, Hee youn
AU - Kang, Dahyun
AU - Hong, Soon Cheol
AU - Cho, Geum-Joon
AU - Oh, Min Jeong
AU - Kim, Hai Joong
PY - 2017/5/9
Y1 - 2017/5/9
N2 - Objective To investigate whether the uterine cervix excision is associated with preterm birth in female mice. Study Design Sexually mature female C57BL/6 mice ( n = 40) were randomly divided into four groups (A, sham; B, cervical excision; C, lipopolysaccharide, 100 µg intrauterine injection; and D, cervical excision + lipopolysaccharide injection), with 10 mice per group. Three weeks after cervical excision, timed mating was performed. On gestational day 16, lipopolysaccharide was injected between the first and second horns of the right uterus near the cervix. The uterine cervix was obtained after delivery and was histologically analyzed. Results The mean gestational period in group D was significantly lower than those in the other groups (17, 19.5, 19, and 18.2 days in groups D, A, B, and C, respectively; p = 0.034). The cervical length was shorter in the cervical excision groups ( p = 0.004). The muscle-to-collagen ratio in the proximal cervix was higher in group D ( p = 0.037). Conclusion Prepregnancy cervical excision and subsequent lipopolysaccharide injection showed a high rate of preterm birth, which was higher than the known lipopolysaccharide injection related preterm birth rate. Prepregnancy cervical excision appears to have additive effects with inflammation in inducing preterm birth, which are associated with the relative muscular component amount.
AB - Objective To investigate whether the uterine cervix excision is associated with preterm birth in female mice. Study Design Sexually mature female C57BL/6 mice ( n = 40) were randomly divided into four groups (A, sham; B, cervical excision; C, lipopolysaccharide, 100 µg intrauterine injection; and D, cervical excision + lipopolysaccharide injection), with 10 mice per group. Three weeks after cervical excision, timed mating was performed. On gestational day 16, lipopolysaccharide was injected between the first and second horns of the right uterus near the cervix. The uterine cervix was obtained after delivery and was histologically analyzed. Results The mean gestational period in group D was significantly lower than those in the other groups (17, 19.5, 19, and 18.2 days in groups D, A, B, and C, respectively; p = 0.034). The cervical length was shorter in the cervical excision groups ( p = 0.004). The muscle-to-collagen ratio in the proximal cervix was higher in group D ( p = 0.037). Conclusion Prepregnancy cervical excision and subsequent lipopolysaccharide injection showed a high rate of preterm birth, which was higher than the known lipopolysaccharide injection related preterm birth rate. Prepregnancy cervical excision appears to have additive effects with inflammation in inducing preterm birth, which are associated with the relative muscular component amount.
KW - excision
KW - infection
KW - mouse model
KW - preterm birth
KW - uterine cervix
UR - http://www.scopus.com/inward/record.url?scp=85020426823&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85020426823&partnerID=8YFLogxK
U2 - 10.1055/s-0037-1603816
DO - 10.1055/s-0037-1603816
M3 - Article
C2 - 28591903
AN - SCOPUS:85020426823
JO - American Journal of Perinatology
JF - American Journal of Perinatology
SN - 0735-1631
ER -