Relationship between Partial Uterine Cervical Tissue Excision and Preterm Birth

An Experimental Animal Study

Ki Hoon Ahn, Hyun Chul Jeong, Hee youn Kim, Dahyun Kang, Soon Cheol Hong, Geum-Joon Cho, Min Jeong Oh, Hai Joong Kim

Research output: Contribution to journalArticle

Abstract

Objective To investigate whether the uterine cervix excision is associated with preterm birth in female mice. Study Design Sexually mature female C57BL/6 mice ( n = 40) were randomly divided into four groups (A, sham; B, cervical excision; C, lipopolysaccharide, 100 µg intrauterine injection; and D, cervical excision + lipopolysaccharide injection), with 10 mice per group. Three weeks after cervical excision, timed mating was performed. On gestational day 16, lipopolysaccharide was injected between the first and second horns of the right uterus near the cervix. The uterine cervix was obtained after delivery and was histologically analyzed. Results The mean gestational period in group D was significantly lower than those in the other groups (17, 19.5, 19, and 18.2 days in groups D, A, B, and C, respectively; p = 0.034). The cervical length was shorter in the cervical excision groups ( p = 0.004). The muscle-to-collagen ratio in the proximal cervix was higher in group D ( p = 0.037). Conclusion Prepregnancy cervical excision and subsequent lipopolysaccharide injection showed a high rate of preterm birth, which was higher than the known lipopolysaccharide injection related preterm birth rate. Prepregnancy cervical excision appears to have additive effects with inflammation in inducing preterm birth, which are associated with the relative muscular component amount.

Original languageEnglish
JournalAmerican Journal of Perinatology
DOIs
Publication statusAccepted/In press - 2017 May 9

Fingerprint

Premature Birth
Lipopolysaccharides
Cervix Uteri
Injections
Birth Rate
Inbred C57BL Mouse
Collagen
Inflammation
Muscles

Keywords

  • excision
  • infection
  • mouse model
  • preterm birth
  • uterine cervix

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynaecology

Cite this

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title = "Relationship between Partial Uterine Cervical Tissue Excision and Preterm Birth: An Experimental Animal Study",
abstract = "Objective To investigate whether the uterine cervix excision is associated with preterm birth in female mice. Study Design Sexually mature female C57BL/6 mice ( n = 40) were randomly divided into four groups (A, sham; B, cervical excision; C, lipopolysaccharide, 100 µg intrauterine injection; and D, cervical excision + lipopolysaccharide injection), with 10 mice per group. Three weeks after cervical excision, timed mating was performed. On gestational day 16, lipopolysaccharide was injected between the first and second horns of the right uterus near the cervix. The uterine cervix was obtained after delivery and was histologically analyzed. Results The mean gestational period in group D was significantly lower than those in the other groups (17, 19.5, 19, and 18.2 days in groups D, A, B, and C, respectively; p = 0.034). The cervical length was shorter in the cervical excision groups ( p = 0.004). The muscle-to-collagen ratio in the proximal cervix was higher in group D ( p = 0.037). Conclusion Prepregnancy cervical excision and subsequent lipopolysaccharide injection showed a high rate of preterm birth, which was higher than the known lipopolysaccharide injection related preterm birth rate. Prepregnancy cervical excision appears to have additive effects with inflammation in inducing preterm birth, which are associated with the relative muscular component amount.",
keywords = "excision, infection, mouse model, preterm birth, uterine cervix",
author = "Ahn, {Ki Hoon} and Jeong, {Hyun Chul} and Kim, {Hee youn} and Dahyun Kang and Hong, {Soon Cheol} and Geum-Joon Cho and Oh, {Min Jeong} and Kim, {Hai Joong}",
year = "2017",
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language = "English",
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T1 - Relationship between Partial Uterine Cervical Tissue Excision and Preterm Birth

T2 - An Experimental Animal Study

AU - Ahn, Ki Hoon

AU - Jeong, Hyun Chul

AU - Kim, Hee youn

AU - Kang, Dahyun

AU - Hong, Soon Cheol

AU - Cho, Geum-Joon

AU - Oh, Min Jeong

AU - Kim, Hai Joong

PY - 2017/5/9

Y1 - 2017/5/9

N2 - Objective To investigate whether the uterine cervix excision is associated with preterm birth in female mice. Study Design Sexually mature female C57BL/6 mice ( n = 40) were randomly divided into four groups (A, sham; B, cervical excision; C, lipopolysaccharide, 100 µg intrauterine injection; and D, cervical excision + lipopolysaccharide injection), with 10 mice per group. Three weeks after cervical excision, timed mating was performed. On gestational day 16, lipopolysaccharide was injected between the first and second horns of the right uterus near the cervix. The uterine cervix was obtained after delivery and was histologically analyzed. Results The mean gestational period in group D was significantly lower than those in the other groups (17, 19.5, 19, and 18.2 days in groups D, A, B, and C, respectively; p = 0.034). The cervical length was shorter in the cervical excision groups ( p = 0.004). The muscle-to-collagen ratio in the proximal cervix was higher in group D ( p = 0.037). Conclusion Prepregnancy cervical excision and subsequent lipopolysaccharide injection showed a high rate of preterm birth, which was higher than the known lipopolysaccharide injection related preterm birth rate. Prepregnancy cervical excision appears to have additive effects with inflammation in inducing preterm birth, which are associated with the relative muscular component amount.

AB - Objective To investigate whether the uterine cervix excision is associated with preterm birth in female mice. Study Design Sexually mature female C57BL/6 mice ( n = 40) were randomly divided into four groups (A, sham; B, cervical excision; C, lipopolysaccharide, 100 µg intrauterine injection; and D, cervical excision + lipopolysaccharide injection), with 10 mice per group. Three weeks after cervical excision, timed mating was performed. On gestational day 16, lipopolysaccharide was injected between the first and second horns of the right uterus near the cervix. The uterine cervix was obtained after delivery and was histologically analyzed. Results The mean gestational period in group D was significantly lower than those in the other groups (17, 19.5, 19, and 18.2 days in groups D, A, B, and C, respectively; p = 0.034). The cervical length was shorter in the cervical excision groups ( p = 0.004). The muscle-to-collagen ratio in the proximal cervix was higher in group D ( p = 0.037). Conclusion Prepregnancy cervical excision and subsequent lipopolysaccharide injection showed a high rate of preterm birth, which was higher than the known lipopolysaccharide injection related preterm birth rate. Prepregnancy cervical excision appears to have additive effects with inflammation in inducing preterm birth, which are associated with the relative muscular component amount.

KW - excision

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KW - uterine cervix

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