Relationships between molecular mobility, fibrillogenesis of collagen molecules, and the inflammatory response

An experimental study in vitro and in vivo

Kwangwoo Nam, Ji-Hun Seo, Tsuyoshi Kimura, Nobuhiko Yui, Akio Kishida

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study was designed to investigate the in vitro adsorption and fibrillogenesis of collagen on a surface with dynamic properties and to investigate how this surface affected the inflammatory response in vivo. Investigation of collagen-surface interactions is directly related to the control of wound healing where collagen adsorption, fibrillization, deposition, and maturation occur. ABA-type block copolymers, composed of polyrotaxane (which possesses α-cyclodextrin threaded along poly(ethylene glycol)) and hydrophobic terminal segments, were used to prepare mobile surfaces with representative dynamic properties. Analyses using a quartz crystal microbalance with dissipation monitoring (QCM-D) indicated that increasing the mobility of the polymer on the surface led to the formation of a soft collagen layer. The collagens in this layer had rearranged, leading to the formation of thicker collagen fibrils by lateral aggregation. When a surface with a high molecular mobility was subcutaneously implanted into rats, collagen rearrangement occurred leading to suppression of macrophage recruitment at the interface and the formation of a fibrotic capsule around the implant. These results suggest that surface mobility on an implant is an important parameter for normal wound healing.

Original languageEnglish
Pages (from-to)16-25
Number of pages10
JournalJournal of Colloid and Interface Science
Volume433
DOIs
Publication statusPublished - 2014 Nov 1
Externally publishedYes

Fingerprint

Collagen
Molecules
Wound Healing
Adsorption
Quartz Crystal Microbalance Techniques
Ethylene Glycol
Macrophages
Quartz crystal microbalances
Cyclodextrins
In Vitro Techniques
Polyethylene glycols
Block copolymers
Capsules
Rats
Polymers
Agglomeration
Monitoring

Keywords

  • Collagen
  • Fibrillogenesis
  • Inflammatory response
  • Molecular mobility
  • Polyrotaxane

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Colloid and Surface Chemistry
  • Medicine(all)

Cite this

Relationships between molecular mobility, fibrillogenesis of collagen molecules, and the inflammatory response : An experimental study in vitro and in vivo. / Nam, Kwangwoo; Seo, Ji-Hun; Kimura, Tsuyoshi; Yui, Nobuhiko; Kishida, Akio.

In: Journal of Colloid and Interface Science, Vol. 433, 01.11.2014, p. 16-25.

Research output: Contribution to journalArticle

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