Relationships between molecular mobility, fibrillogenesis of collagen molecules, and the inflammatory response: An experimental study in vitro and in vivo

Kwangwoo Nam, Ji-Hun Seo, Tsuyoshi Kimura, Nobuhiko Yui, Akio Kishida

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study was designed to investigate the in vitro adsorption and fibrillogenesis of collagen on a surface with dynamic properties and to investigate how this surface affected the inflammatory response in vivo. Investigation of collagen-surface interactions is directly related to the control of wound healing where collagen adsorption, fibrillization, deposition, and maturation occur. ABA-type block copolymers, composed of polyrotaxane (which possesses α-cyclodextrin threaded along poly(ethylene glycol)) and hydrophobic terminal segments, were used to prepare mobile surfaces with representative dynamic properties. Analyses using a quartz crystal microbalance with dissipation monitoring (QCM-D) indicated that increasing the mobility of the polymer on the surface led to the formation of a soft collagen layer. The collagens in this layer had rearranged, leading to the formation of thicker collagen fibrils by lateral aggregation. When a surface with a high molecular mobility was subcutaneously implanted into rats, collagen rearrangement occurred leading to suppression of macrophage recruitment at the interface and the formation of a fibrotic capsule around the implant. These results suggest that surface mobility on an implant is an important parameter for normal wound healing.

Original languageEnglish
Pages (from-to)16-25
Number of pages10
JournalJournal of Colloid and Interface Science
Volume433
DOIs
Publication statusPublished - 2014 Nov 1
Externally publishedYes

Keywords

  • Collagen
  • Fibrillogenesis
  • Inflammatory response
  • Molecular mobility
  • Polyrotaxane

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Colloid and Surface Chemistry
  • Medicine(all)

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