Abstract
This study was designed to investigate the in vitro adsorption and fibrillogenesis of collagen on a surface with dynamic properties and to investigate how this surface affected the inflammatory response in vivo. Investigation of collagen-surface interactions is directly related to the control of wound healing where collagen adsorption, fibrillization, deposition, and maturation occur. ABA-type block copolymers, composed of polyrotaxane (which possesses α-cyclodextrin threaded along poly(ethylene glycol)) and hydrophobic terminal segments, were used to prepare mobile surfaces with representative dynamic properties. Analyses using a quartz crystal microbalance with dissipation monitoring (QCM-D) indicated that increasing the mobility of the polymer on the surface led to the formation of a soft collagen layer. The collagens in this layer had rearranged, leading to the formation of thicker collagen fibrils by lateral aggregation. When a surface with a high molecular mobility was subcutaneously implanted into rats, collagen rearrangement occurred leading to suppression of macrophage recruitment at the interface and the formation of a fibrotic capsule around the implant. These results suggest that surface mobility on an implant is an important parameter for normal wound healing.
| Original language | English |
|---|---|
| Pages (from-to) | 16-25 |
| Number of pages | 10 |
| Journal | Journal of Colloid and Interface Science |
| Volume | 433 |
| DOIs | |
| Publication status | Published - 2014 Nov 1 |
| Externally published | Yes |
Fingerprint
Keywords
- Collagen
- Fibrillogenesis
- Inflammatory response
- Molecular mobility
- Polyrotaxane
ASJC Scopus subject areas
- Surfaces, Coatings and Films
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Colloid and Surface Chemistry
- Medicine(all)
Cite this
Relationships between molecular mobility, fibrillogenesis of collagen molecules, and the inflammatory response : An experimental study in vitro and in vivo. / Nam, Kwangwoo; Seo, Ji-Hun; Kimura, Tsuyoshi; Yui, Nobuhiko; Kishida, Akio.
In: Journal of Colloid and Interface Science, Vol. 433, 01.11.2014, p. 16-25.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relationships between molecular mobility, fibrillogenesis of collagen molecules, and the inflammatory response
T2 - An experimental study in vitro and in vivo
AU - Nam, Kwangwoo
AU - Seo, Ji-Hun
AU - Kimura, Tsuyoshi
AU - Yui, Nobuhiko
AU - Kishida, Akio
PY - 2014/11/1
Y1 - 2014/11/1
N2 - This study was designed to investigate the in vitro adsorption and fibrillogenesis of collagen on a surface with dynamic properties and to investigate how this surface affected the inflammatory response in vivo. Investigation of collagen-surface interactions is directly related to the control of wound healing where collagen adsorption, fibrillization, deposition, and maturation occur. ABA-type block copolymers, composed of polyrotaxane (which possesses α-cyclodextrin threaded along poly(ethylene glycol)) and hydrophobic terminal segments, were used to prepare mobile surfaces with representative dynamic properties. Analyses using a quartz crystal microbalance with dissipation monitoring (QCM-D) indicated that increasing the mobility of the polymer on the surface led to the formation of a soft collagen layer. The collagens in this layer had rearranged, leading to the formation of thicker collagen fibrils by lateral aggregation. When a surface with a high molecular mobility was subcutaneously implanted into rats, collagen rearrangement occurred leading to suppression of macrophage recruitment at the interface and the formation of a fibrotic capsule around the implant. These results suggest that surface mobility on an implant is an important parameter for normal wound healing.
AB - This study was designed to investigate the in vitro adsorption and fibrillogenesis of collagen on a surface with dynamic properties and to investigate how this surface affected the inflammatory response in vivo. Investigation of collagen-surface interactions is directly related to the control of wound healing where collagen adsorption, fibrillization, deposition, and maturation occur. ABA-type block copolymers, composed of polyrotaxane (which possesses α-cyclodextrin threaded along poly(ethylene glycol)) and hydrophobic terminal segments, were used to prepare mobile surfaces with representative dynamic properties. Analyses using a quartz crystal microbalance with dissipation monitoring (QCM-D) indicated that increasing the mobility of the polymer on the surface led to the formation of a soft collagen layer. The collagens in this layer had rearranged, leading to the formation of thicker collagen fibrils by lateral aggregation. When a surface with a high molecular mobility was subcutaneously implanted into rats, collagen rearrangement occurred leading to suppression of macrophage recruitment at the interface and the formation of a fibrotic capsule around the implant. These results suggest that surface mobility on an implant is an important parameter for normal wound healing.
KW - Collagen
KW - Fibrillogenesis
KW - Inflammatory response
KW - Molecular mobility
KW - Polyrotaxane
UR - http://www.scopus.com/inward/record.url?scp=84905690776&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84905690776&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2014.06.017
DO - 10.1016/j.jcis.2014.06.017
M3 - Article
C2 - 25112908
AN - SCOPUS:84905690776
VL - 433
SP - 16
EP - 25
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
SN - 0021-9797
ER -