Remote Manipulation of Ligand Nano-Oscillations Regulates Adhesion and Polarization of Macrophages in Vivo

Heemin Kang, Sungkyu Kim, Dexter Siu Hong Wong, Hee Joon Jung, Sien Lin, Kaijie Zou, Rui Li, Gang Li, Vinayak P. Dravid, Liming Bian

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Macrophages play crucial roles in various immune-related responses, such as host defense, wound healing, disease progression, and tissue regeneration. Macrophages perform distinct and dynamic functions in vivo, depending on their polarization states, such as the pro-inflammatory M1 phenotype and pro-healing M2 phenotype. Remote manipulation of the adhesion of host macrophages to the implants and their subsequent polarization in vivo can be an attractive strategy to control macrophage polarization-specific functions but has rarely been achieved. In this study, we grafted RGD ligand-bearing superparamagnetic iron oxide nanoparticles (SPIONs) to a planar matrix via a long flexible linker. We characterized the nanoscale motion of the RGD-bearing SPIONs grafted to the matrix, in real time by in situ magnetic scanning transmission electron microscopy (STEM) and in situ atomic force microscopy. The magnetic field was applied at various oscillation frequencies to manipulate the frequency-dependent ligand nano-oscillation speeds of the RGD-bearing SPIONs. We demonstrate that a low oscillation frequency of the magnetic field stimulated the adhesion and M2 polarization of macrophages, whereas a high oscillation frequency suppressed the adhesion of macrophages but promoted their M1 polarization, both in vitro and in vivo. Macrophage adhesion was also temporally regulated by switching between the low and high frequencies of the oscillating magnetic field. To the best of our knowledge, this is the first demonstration of the remote manipulation of the adhesion and polarization phenotype of macrophages, both in vitro and in vivo. Our system offers the promising potential to manipulate host immune responses to implanted biomaterials, including inflammation or tissue reparative processes, by regulating macrophage adhesion and polarization.

Original languageEnglish
Pages (from-to)6415-6427
Number of pages13
JournalNano Letters
Volume17
Issue number10
DOIs
Publication statusPublished - 2017 Oct 11
Externally publishedYes

Fingerprint

macrophages
Macrophages
manipulators
adhesion
Adhesion
Ligands
Polarization
oscillations
ligands
Bearings (structural)
polarization
phenotype
Iron oxides
iron oxides
Magnetic fields
Nanoparticles
nanoparticles
magnetic fields
wound healing
Tissue regeneration

Keywords

  • Ligand nano-oscillations
  • macrophage adhesion
  • macrophage polarization
  • remote manipulation
  • SPION

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Remote Manipulation of Ligand Nano-Oscillations Regulates Adhesion and Polarization of Macrophages in Vivo. / Kang, Heemin; Kim, Sungkyu; Wong, Dexter Siu Hong; Jung, Hee Joon; Lin, Sien; Zou, Kaijie; Li, Rui; Li, Gang; Dravid, Vinayak P.; Bian, Liming.

In: Nano Letters, Vol. 17, No. 10, 11.10.2017, p. 6415-6427.

Research output: Contribution to journalArticle

Kang, H, Kim, S, Wong, DSH, Jung, HJ, Lin, S, Zou, K, Li, R, Li, G, Dravid, VP & Bian, L 2017, 'Remote Manipulation of Ligand Nano-Oscillations Regulates Adhesion and Polarization of Macrophages in Vivo', Nano Letters, vol. 17, no. 10, pp. 6415-6427. https://doi.org/10.1021/acs.nanolett.7b03405
Kang, Heemin ; Kim, Sungkyu ; Wong, Dexter Siu Hong ; Jung, Hee Joon ; Lin, Sien ; Zou, Kaijie ; Li, Rui ; Li, Gang ; Dravid, Vinayak P. ; Bian, Liming. / Remote Manipulation of Ligand Nano-Oscillations Regulates Adhesion and Polarization of Macrophages in Vivo. In: Nano Letters. 2017 ; Vol. 17, No. 10. pp. 6415-6427.
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