Abstract
The authors investigated whether a remote postconditioning (remote post-con) procedure attenuated skeletal muscle ischemia/reperfusion (I/R) injury. We determined the optimal protocol of remote post-con and investigated its mechanism. Ischemia was induced for 3 hours in rat left hindlimb and three protocols of remote post-con were applied in right hindlimb just before the end of ischemia. The first (10-second group) involved two cycles of 10 seconds of occlusion followed by 10 seconds of reperfusion. The second (5-minute group) involved two cycles of 5 minutes of occlusion/reperfusion. The third (10-minute group) involved two cycles of 10 minutes of occlusion/reperfusion. In 5- and 10-minute groups, wet/dry ratio and muscle fiber edema were significantly lower than control group. Muscle contractility was preserved in 5- and 10-minute groups. An injection of 5-hydroxydecanoate (a specific blocker of mitochondrial ATP-sensitive K+ [mKATP] channels) impaired this effect. This study demonstrates that remote post-con preserves muscle contractility and reduces tissue edema and necrosis, possibly through the activation of mK ATP channels. We suggest that two cycles of 5 minutes of occlusion followed by 5 minutes of reperfusion are optimal protocols of remote post-con in skeletal muscle I/R injury.
| Original language | English |
|---|---|
| Pages (from-to) | 571-578 |
| Number of pages | 8 |
| Journal | Journal of Reconstructive Microsurgery |
| Volume | 29 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2013 Jun 14 |
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Keywords
- ATP-sensitive K channel
- Ischemia/reperfusion injury
- postconditioning
- remote postconditioning
ASJC Scopus subject areas
- Surgery
Cite this
Remote postconditioning attenuates ischemia/reperfusion injury in rat skeletal muscle through mitochondrial ATP-sensitive K+ channel-dependent mechanism. / Park, Jong Woong; Lee, Jungil; Wook Nha, Kyung; Suh, Jinsoo; Choo, Sukkyu; Park, Jung-Ho.
In: Journal of Reconstructive Microsurgery, Vol. 29, No. 9, 14.06.2013, p. 571-578.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Remote postconditioning attenuates ischemia/reperfusion injury in rat skeletal muscle through mitochondrial ATP-sensitive K+ channel-dependent mechanism
AU - Park, Jong Woong
AU - Lee, Jungil
AU - Wook Nha, Kyung
AU - Suh, Jinsoo
AU - Choo, Sukkyu
AU - Park, Jung-Ho
PY - 2013/6/14
Y1 - 2013/6/14
N2 - The authors investigated whether a remote postconditioning (remote post-con) procedure attenuated skeletal muscle ischemia/reperfusion (I/R) injury. We determined the optimal protocol of remote post-con and investigated its mechanism. Ischemia was induced for 3 hours in rat left hindlimb and three protocols of remote post-con were applied in right hindlimb just before the end of ischemia. The first (10-second group) involved two cycles of 10 seconds of occlusion followed by 10 seconds of reperfusion. The second (5-minute group) involved two cycles of 5 minutes of occlusion/reperfusion. The third (10-minute group) involved two cycles of 10 minutes of occlusion/reperfusion. In 5- and 10-minute groups, wet/dry ratio and muscle fiber edema were significantly lower than control group. Muscle contractility was preserved in 5- and 10-minute groups. An injection of 5-hydroxydecanoate (a specific blocker of mitochondrial ATP-sensitive K+ [mKATP] channels) impaired this effect. This study demonstrates that remote post-con preserves muscle contractility and reduces tissue edema and necrosis, possibly through the activation of mK ATP channels. We suggest that two cycles of 5 minutes of occlusion followed by 5 minutes of reperfusion are optimal protocols of remote post-con in skeletal muscle I/R injury.
AB - The authors investigated whether a remote postconditioning (remote post-con) procedure attenuated skeletal muscle ischemia/reperfusion (I/R) injury. We determined the optimal protocol of remote post-con and investigated its mechanism. Ischemia was induced for 3 hours in rat left hindlimb and three protocols of remote post-con were applied in right hindlimb just before the end of ischemia. The first (10-second group) involved two cycles of 10 seconds of occlusion followed by 10 seconds of reperfusion. The second (5-minute group) involved two cycles of 5 minutes of occlusion/reperfusion. The third (10-minute group) involved two cycles of 10 minutes of occlusion/reperfusion. In 5- and 10-minute groups, wet/dry ratio and muscle fiber edema were significantly lower than control group. Muscle contractility was preserved in 5- and 10-minute groups. An injection of 5-hydroxydecanoate (a specific blocker of mitochondrial ATP-sensitive K+ [mKATP] channels) impaired this effect. This study demonstrates that remote post-con preserves muscle contractility and reduces tissue edema and necrosis, possibly through the activation of mK ATP channels. We suggest that two cycles of 5 minutes of occlusion followed by 5 minutes of reperfusion are optimal protocols of remote post-con in skeletal muscle I/R injury.
KW - ATP-sensitive K channel
KW - Ischemia/reperfusion injury
KW - postconditioning
KW - remote postconditioning
UR - http://www.scopus.com/inward/record.url?scp=84885955064&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885955064&partnerID=8YFLogxK
U2 - 10.1055/s-0033-1348037
DO - 10.1055/s-0033-1348037
M3 - Article
C2 - 23757151
AN - SCOPUS:84885955064
VL - 29
SP - 571
EP - 578
JO - Journal of Reconstructive Microsurgery
JF - Journal of Reconstructive Microsurgery
SN - 0743-684X
IS - 9
ER -