Removal of Endotoxins and Nucleic Acids Using Submicron-sized Polymeric Particles

Chan Wha Kim, C. William Christopher, Chokyun Rha

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Submicron-sized polymeric particles (SSPP) were used to remove nucleic acids and endotoxins from cell lysates. The positively charged SSPP selectively adsorb nucleic acids and endotoxins and form complexes with them. The complexes can be easily removed by sedimentation or centrifugation. The removal of nucleic acids and endotoxins using SSPP also can be accomplished in the presence of cell and cell debris. Therefore, nucleic acids and endotoxins can be removed in an initial step of the down-stream processes. In bakers yeast and E. coli lysate systems, the level of DNA could be reduced more than three orders of magnitudes and endotoxins more than seven orders of magnitudes concurrently with the cell debris removal process using SSPP.

Original languageEnglish
Pages (from-to)189-193
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume6
Issue number3
Publication statusPublished - 1996 Jun 1

Fingerprint

Nucleic acids
Endotoxins
Nucleic Acids
Debris
Centrifugation
Sedimentation
Yeast
Escherichia coli
DNA
Saccharomyces cerevisiae

Keywords

  • Clarification
  • Polymeric particle
  • Pyrogen
  • Removal of endotoxin
  • Removal of nucleic acid
  • Submicron-sized polymeric particles

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Removal of Endotoxins and Nucleic Acids Using Submicron-sized Polymeric Particles. / Kim, Chan Wha; Christopher, C. William; Rha, Chokyun.

In: Journal of Microbiology and Biotechnology, Vol. 6, No. 3, 01.06.1996, p. 189-193.

Research output: Contribution to journalArticle

@article{24de40d1dd46487881a3b0f6f9f74ec5,
title = "Removal of Endotoxins and Nucleic Acids Using Submicron-sized Polymeric Particles",
abstract = "Submicron-sized polymeric particles (SSPP) were used to remove nucleic acids and endotoxins from cell lysates. The positively charged SSPP selectively adsorb nucleic acids and endotoxins and form complexes with them. The complexes can be easily removed by sedimentation or centrifugation. The removal of nucleic acids and endotoxins using SSPP also can be accomplished in the presence of cell and cell debris. Therefore, nucleic acids and endotoxins can be removed in an initial step of the down-stream processes. In bakers yeast and E. coli lysate systems, the level of DNA could be reduced more than three orders of magnitudes and endotoxins more than seven orders of magnitudes concurrently with the cell debris removal process using SSPP.",
keywords = "Clarification, Polymeric particle, Pyrogen, Removal of endotoxin, Removal of nucleic acid, Submicron-sized polymeric particles",
author = "Kim, {Chan Wha} and Christopher, {C. William} and Chokyun Rha",
year = "1996",
month = "6",
day = "1",
language = "English",
volume = "6",
pages = "189--193",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "3",

}

TY - JOUR

T1 - Removal of Endotoxins and Nucleic Acids Using Submicron-sized Polymeric Particles

AU - Kim, Chan Wha

AU - Christopher, C. William

AU - Rha, Chokyun

PY - 1996/6/1

Y1 - 1996/6/1

N2 - Submicron-sized polymeric particles (SSPP) were used to remove nucleic acids and endotoxins from cell lysates. The positively charged SSPP selectively adsorb nucleic acids and endotoxins and form complexes with them. The complexes can be easily removed by sedimentation or centrifugation. The removal of nucleic acids and endotoxins using SSPP also can be accomplished in the presence of cell and cell debris. Therefore, nucleic acids and endotoxins can be removed in an initial step of the down-stream processes. In bakers yeast and E. coli lysate systems, the level of DNA could be reduced more than three orders of magnitudes and endotoxins more than seven orders of magnitudes concurrently with the cell debris removal process using SSPP.

AB - Submicron-sized polymeric particles (SSPP) were used to remove nucleic acids and endotoxins from cell lysates. The positively charged SSPP selectively adsorb nucleic acids and endotoxins and form complexes with them. The complexes can be easily removed by sedimentation or centrifugation. The removal of nucleic acids and endotoxins using SSPP also can be accomplished in the presence of cell and cell debris. Therefore, nucleic acids and endotoxins can be removed in an initial step of the down-stream processes. In bakers yeast and E. coli lysate systems, the level of DNA could be reduced more than three orders of magnitudes and endotoxins more than seven orders of magnitudes concurrently with the cell debris removal process using SSPP.

KW - Clarification

KW - Polymeric particle

KW - Pyrogen

KW - Removal of endotoxin

KW - Removal of nucleic acid

KW - Submicron-sized polymeric particles

UR - http://www.scopus.com/inward/record.url?scp=0013428713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0013428713&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 189

EP - 193

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 3

ER -