Renal tubular damage detected by dynamic micro-MRI with a dendrimer-based magnetic resonance contrast agent

Hisataka Kobayashi, Satomi Kawamoto, Sang Kyung Jo, Noriko Sato, Tsuneo Saga, Akira Hiraga, Junji Konishi, Susan Hu, Kaori Togashi, Martin W. Brechbiel, Robert A. Star

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Background. A noninvasive technique to evaluate the structure and function of the kidney would be useful to investigate renal diseases, especially acute renal failure. We have developed a novel technique to visualize functional micro-magnetic resonance (MR) images of the mouse kidney with a dendrimer-based macromolecular renal MR contrast agent. Method. Mice were injected with cisplatin or vehicle, then examined three days later by contrast-enhanced, dynamic high-resolution micro-MRI with 160 μm spatial resolution using a 1.5 T clinical MRI unit, a surface coil, and the renal contrast agent G4D-(1B4M-Gd)64. Results. The cortex and outer stripe of the outer medulla of the mouse kidney were clearly visualized in the normal mice. In animals treated with cisplatin, the gradation of tubular damage as assessed by contrast enhanced dynamic MRI correlated with renal function. Conclusion. Contrast-enhanced, dynamic high-resolution micro-MRI with a novel dendrimer-based macromolecular renal MR contrast agent can be a powerful tool for in vivo observation of renal structural and functional damage.

Original languageEnglish
Pages (from-to)1980-1985
Number of pages6
JournalKidney International
Volume61
Issue number6
DOIs
Publication statusPublished - 2002 Jan 1
Externally publishedYes

Fingerprint

Dendrimers
Contrast Media
Magnetic Resonance Spectroscopy
Kidney
Cisplatin
Renal Agents
Kidney Medulla
Acute Kidney Injury
Observation

Keywords

  • Cisplatin
  • Contrast agent
  • Dendrimer
  • Magnetic resonance imaging
  • Non-invasive imaging
  • Proximal tubules

ASJC Scopus subject areas

  • Nephrology

Cite this

Renal tubular damage detected by dynamic micro-MRI with a dendrimer-based magnetic resonance contrast agent. / Kobayashi, Hisataka; Kawamoto, Satomi; Jo, Sang Kyung; Sato, Noriko; Saga, Tsuneo; Hiraga, Akira; Konishi, Junji; Hu, Susan; Togashi, Kaori; Brechbiel, Martin W.; Star, Robert A.

In: Kidney International, Vol. 61, No. 6, 01.01.2002, p. 1980-1985.

Research output: Contribution to journalArticle

Kobayashi, H, Kawamoto, S, Jo, SK, Sato, N, Saga, T, Hiraga, A, Konishi, J, Hu, S, Togashi, K, Brechbiel, MW & Star, RA 2002, 'Renal tubular damage detected by dynamic micro-MRI with a dendrimer-based magnetic resonance contrast agent', Kidney International, vol. 61, no. 6, pp. 1980-1985. https://doi.org/10.1046/j.1523-1755.2002.00364.x
Kobayashi, Hisataka ; Kawamoto, Satomi ; Jo, Sang Kyung ; Sato, Noriko ; Saga, Tsuneo ; Hiraga, Akira ; Konishi, Junji ; Hu, Susan ; Togashi, Kaori ; Brechbiel, Martin W. ; Star, Robert A. / Renal tubular damage detected by dynamic micro-MRI with a dendrimer-based magnetic resonance contrast agent. In: Kidney International. 2002 ; Vol. 61, No. 6. pp. 1980-1985.
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