Abstract
Background. A noninvasive technique to evaluate the structure and function of the kidney would be useful to investigate renal diseases, especially acute renal failure. We have developed a novel technique to visualize functional micro-magnetic resonance (MR) images of the mouse kidney with a dendrimer-based macromolecular renal MR contrast agent. Method. Mice were injected with cisplatin or vehicle, then examined three days later by contrast-enhanced, dynamic high-resolution micro-MRI with 160 μm spatial resolution using a 1.5 T clinical MRI unit, a surface coil, and the renal contrast agent G4D-(1B4M-Gd)64. Results. The cortex and outer stripe of the outer medulla of the mouse kidney were clearly visualized in the normal mice. In animals treated with cisplatin, the gradation of tubular damage as assessed by contrast enhanced dynamic MRI correlated with renal function. Conclusion. Contrast-enhanced, dynamic high-resolution micro-MRI with a novel dendrimer-based macromolecular renal MR contrast agent can be a powerful tool for in vivo observation of renal structural and functional damage.
| Original language | English |
|---|---|
| Pages (from-to) | 1980-1985 |
| Number of pages | 6 |
| Journal | Kidney International |
| Volume | 61 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2002 Jan 1 |
| Externally published | Yes |
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Keywords
- Cisplatin
- Contrast agent
- Dendrimer
- Magnetic resonance imaging
- Non-invasive imaging
- Proximal tubules
ASJC Scopus subject areas
- Nephrology
Cite this
Renal tubular damage detected by dynamic micro-MRI with a dendrimer-based magnetic resonance contrast agent. / Kobayashi, Hisataka; Kawamoto, Satomi; Jo, Sang Kyung; Sato, Noriko; Saga, Tsuneo; Hiraga, Akira; Konishi, Junji; Hu, Susan; Togashi, Kaori; Brechbiel, Martin W.; Star, Robert A.
In: Kidney International, Vol. 61, No. 6, 01.01.2002, p. 1980-1985.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Renal tubular damage detected by dynamic micro-MRI with a dendrimer-based magnetic resonance contrast agent
AU - Kobayashi, Hisataka
AU - Kawamoto, Satomi
AU - Jo, Sang Kyung
AU - Sato, Noriko
AU - Saga, Tsuneo
AU - Hiraga, Akira
AU - Konishi, Junji
AU - Hu, Susan
AU - Togashi, Kaori
AU - Brechbiel, Martin W.
AU - Star, Robert A.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Background. A noninvasive technique to evaluate the structure and function of the kidney would be useful to investigate renal diseases, especially acute renal failure. We have developed a novel technique to visualize functional micro-magnetic resonance (MR) images of the mouse kidney with a dendrimer-based macromolecular renal MR contrast agent. Method. Mice were injected with cisplatin or vehicle, then examined three days later by contrast-enhanced, dynamic high-resolution micro-MRI with 160 μm spatial resolution using a 1.5 T clinical MRI unit, a surface coil, and the renal contrast agent G4D-(1B4M-Gd)64. Results. The cortex and outer stripe of the outer medulla of the mouse kidney were clearly visualized in the normal mice. In animals treated with cisplatin, the gradation of tubular damage as assessed by contrast enhanced dynamic MRI correlated with renal function. Conclusion. Contrast-enhanced, dynamic high-resolution micro-MRI with a novel dendrimer-based macromolecular renal MR contrast agent can be a powerful tool for in vivo observation of renal structural and functional damage.
AB - Background. A noninvasive technique to evaluate the structure and function of the kidney would be useful to investigate renal diseases, especially acute renal failure. We have developed a novel technique to visualize functional micro-magnetic resonance (MR) images of the mouse kidney with a dendrimer-based macromolecular renal MR contrast agent. Method. Mice were injected with cisplatin or vehicle, then examined three days later by contrast-enhanced, dynamic high-resolution micro-MRI with 160 μm spatial resolution using a 1.5 T clinical MRI unit, a surface coil, and the renal contrast agent G4D-(1B4M-Gd)64. Results. The cortex and outer stripe of the outer medulla of the mouse kidney were clearly visualized in the normal mice. In animals treated with cisplatin, the gradation of tubular damage as assessed by contrast enhanced dynamic MRI correlated with renal function. Conclusion. Contrast-enhanced, dynamic high-resolution micro-MRI with a novel dendrimer-based macromolecular renal MR contrast agent can be a powerful tool for in vivo observation of renal structural and functional damage.
KW - Cisplatin
KW - Contrast agent
KW - Dendrimer
KW - Magnetic resonance imaging
KW - Non-invasive imaging
KW - Proximal tubules
UR - http://www.scopus.com/inward/record.url?scp=0036103603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036103603&partnerID=8YFLogxK
U2 - 10.1046/j.1523-1755.2002.00364.x
DO - 10.1046/j.1523-1755.2002.00364.x
M3 - Article
C2 - 12028438
AN - SCOPUS:0036103603
VL - 61
SP - 1980
EP - 1985
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 6
ER -