Repair of rabbit ulna segmental bone defect using freshly isolated adipose-derived stromal vascular fraction

Aeree Kim, Dong Hyun Kim, Hae Ryong Song, Won Hwa Kang, Hak Jun Kim, Hong Chul Lim, Dong Woo Cho, Ji Hoon Bae

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background aims. Stromal vascular fractions (SVF) from adipose tissue have heterogeneous cell populations, and include multipotent adipose-derived stem cells. The advantages of using of SVF include the avoidance of an additional culture period, a reduced risk of extensive cell contamination, and cost-effectiveness. Methods. Unilateral 20-mm mid-diaphyseal segmental defects in rabbit ulna were treated with one of the following: polylactic glycolic acid (PLGA) scaffold alone (group 1, control), a PLGA scaffold with undifferentiated SVF cells (group 2), or a PLGA scaffold with osteogenically differentiated SVF cells (group 3). At 8 weeks after implantation, five rabbits in each treatment group were killed to assess bone defect healing by plain radiography, quantitative microcomputed tomography and histology. Results. The SVF cells were well grown on PLGA scaffolds and expressed type I collagen and alkaline phosphatase (ALP). The intensity of ALP and OPN gene expressions in osteogenic medium culture were increased from 14 days to 28 days. In vivo evaluations at 8 weeks showed that treatment of SVF cells with or without osteogenic differentiation resulted in more bone formation in the critically sized segmental defects than PLGA scaffold alone. Osteogenically differentiated SVF cells significantly enhanced bone healing compared with undifferentiated SVF cells. Conclusions. Adipose-derived stromal SVF showed osteogenic potential in vitro. Accordingly, SVF could provide a cell source for bone tissue engineering. However, treatment with uncultured SVF cells on bone healing was not satisfactory in the in vivo animal model.

Original languageEnglish
Pages (from-to)296-305
Number of pages10
JournalCytotherapy
Volume14
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1

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Keywords

  • Adipose tissue
  • Bone
  • Mesenchymal stromal cell
  • Stromal vascular fraction
  • Tissue engineering

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Molecular Medicine

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