Repeated batch production of 1,3-propanediol from biodiesel derived waste glycerol by Klebsiella pneumoniae

Xiaoguang Yang, Dong Sup Kim, Han Suk Choi, Chan Kyum Kim, Laxmi Prasad Thapa, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study, 1,3-propanediol production was achieved from waste glycerol using Klebsiella pneumoniae ATCC 8724 under both batch and repeated batch fermentation. The inhibitory effects of the culture conditions were investigated considering the waste glycerol components. Anaerobic conditions showed better performance than aerobic conditions. Significant inhibitory effects in batch fermentation were observed in the following conditions: above pH 8, 60 g/L of initial glycerol concentration or 2% (w/v) of salts contents. However, improvement in 1,3-propanediol production and cell growth was represented below 1% (w/v) content of salts (NaCl and KCl). The highest yield of 1,3-propandiol using batch fermentation was achieved at 0.77 and 0.65 (mol/mol) with pure and waste glycerol, respectively. In addition, repeated batch fermentation was demonstrated with immobilized cells using waste glycerol. Compared to the first cycle of cultivation, 83% of 1,3-propanediol was obtained after five cycles of batch cultivation. The highest concentration and yield of 1,3-propanediol were respectively achieved 0.64 (mol/mol) and 20 g/L at the second cycle cultivation. The performance of by-products of the repeated batch fermentation with immobilized cells was also investigated for further application.

Original languageEnglish
Pages (from-to)660-669
Number of pages10
JournalChemical Engineering Journal
Volume314
DOIs
Publication statusPublished - 2017

Fingerprint

pneumonia
Biofuels
Biodiesel
Glycerol
Fermentation
fermentation
Salts
Cells
Cell growth
oxic conditions
anoxic conditions
Byproducts
1,3-propanediol
salt

Keywords

  • 1,3-Propanediol
  • Immobilized cell
  • Inhibitory effect
  • Klebsiella pneumoniae
  • Waste glycerol

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Repeated batch production of 1,3-propanediol from biodiesel derived waste glycerol by Klebsiella pneumoniae. / Yang, Xiaoguang; Kim, Dong Sup; Choi, Han Suk; Kim, Chan Kyum; Thapa, Laxmi Prasad; Park, Chulhwan; Kim, Seung Wook.

In: Chemical Engineering Journal, Vol. 314, 2017, p. 660-669.

Research output: Contribution to journalArticle

Yang, Xiaoguang ; Kim, Dong Sup ; Choi, Han Suk ; Kim, Chan Kyum ; Thapa, Laxmi Prasad ; Park, Chulhwan ; Kim, Seung Wook. / Repeated batch production of 1,3-propanediol from biodiesel derived waste glycerol by Klebsiella pneumoniae. In: Chemical Engineering Journal. 2017 ; Vol. 314. pp. 660-669.
@article{537ae1f46e5d4e9896bbad2e25f0923c,
title = "Repeated batch production of 1,3-propanediol from biodiesel derived waste glycerol by Klebsiella pneumoniae",
abstract = "In this study, 1,3-propanediol production was achieved from waste glycerol using Klebsiella pneumoniae ATCC 8724 under both batch and repeated batch fermentation. The inhibitory effects of the culture conditions were investigated considering the waste glycerol components. Anaerobic conditions showed better performance than aerobic conditions. Significant inhibitory effects in batch fermentation were observed in the following conditions: above pH 8, 60 g/L of initial glycerol concentration or 2{\%} (w/v) of salts contents. However, improvement in 1,3-propanediol production and cell growth was represented below 1{\%} (w/v) content of salts (NaCl and KCl). The highest yield of 1,3-propandiol using batch fermentation was achieved at 0.77 and 0.65 (mol/mol) with pure and waste glycerol, respectively. In addition, repeated batch fermentation was demonstrated with immobilized cells using waste glycerol. Compared to the first cycle of cultivation, 83{\%} of 1,3-propanediol was obtained after five cycles of batch cultivation. The highest concentration and yield of 1,3-propanediol were respectively achieved 0.64 (mol/mol) and 20 g/L at the second cycle cultivation. The performance of by-products of the repeated batch fermentation with immobilized cells was also investigated for further application.",
keywords = "1,3-Propanediol, Immobilized cell, Inhibitory effect, Klebsiella pneumoniae, Waste glycerol",
author = "Xiaoguang Yang and Kim, {Dong Sup} and Choi, {Han Suk} and Kim, {Chan Kyum} and Thapa, {Laxmi Prasad} and Chulhwan Park and Kim, {Seung Wook}",
year = "2017",
doi = "10.1016/j.cej.2016.12.029",
language = "English",
volume = "314",
pages = "660--669",
journal = "Chemical Engineering Journal",
issn = "1385-8947",
publisher = "Elsevier",

}

TY - JOUR

T1 - Repeated batch production of 1,3-propanediol from biodiesel derived waste glycerol by Klebsiella pneumoniae

AU - Yang, Xiaoguang

AU - Kim, Dong Sup

AU - Choi, Han Suk

AU - Kim, Chan Kyum

AU - Thapa, Laxmi Prasad

AU - Park, Chulhwan

AU - Kim, Seung Wook

PY - 2017

Y1 - 2017

N2 - In this study, 1,3-propanediol production was achieved from waste glycerol using Klebsiella pneumoniae ATCC 8724 under both batch and repeated batch fermentation. The inhibitory effects of the culture conditions were investigated considering the waste glycerol components. Anaerobic conditions showed better performance than aerobic conditions. Significant inhibitory effects in batch fermentation were observed in the following conditions: above pH 8, 60 g/L of initial glycerol concentration or 2% (w/v) of salts contents. However, improvement in 1,3-propanediol production and cell growth was represented below 1% (w/v) content of salts (NaCl and KCl). The highest yield of 1,3-propandiol using batch fermentation was achieved at 0.77 and 0.65 (mol/mol) with pure and waste glycerol, respectively. In addition, repeated batch fermentation was demonstrated with immobilized cells using waste glycerol. Compared to the first cycle of cultivation, 83% of 1,3-propanediol was obtained after five cycles of batch cultivation. The highest concentration and yield of 1,3-propanediol were respectively achieved 0.64 (mol/mol) and 20 g/L at the second cycle cultivation. The performance of by-products of the repeated batch fermentation with immobilized cells was also investigated for further application.

AB - In this study, 1,3-propanediol production was achieved from waste glycerol using Klebsiella pneumoniae ATCC 8724 under both batch and repeated batch fermentation. The inhibitory effects of the culture conditions were investigated considering the waste glycerol components. Anaerobic conditions showed better performance than aerobic conditions. Significant inhibitory effects in batch fermentation were observed in the following conditions: above pH 8, 60 g/L of initial glycerol concentration or 2% (w/v) of salts contents. However, improvement in 1,3-propanediol production and cell growth was represented below 1% (w/v) content of salts (NaCl and KCl). The highest yield of 1,3-propandiol using batch fermentation was achieved at 0.77 and 0.65 (mol/mol) with pure and waste glycerol, respectively. In addition, repeated batch fermentation was demonstrated with immobilized cells using waste glycerol. Compared to the first cycle of cultivation, 83% of 1,3-propanediol was obtained after five cycles of batch cultivation. The highest concentration and yield of 1,3-propanediol were respectively achieved 0.64 (mol/mol) and 20 g/L at the second cycle cultivation. The performance of by-products of the repeated batch fermentation with immobilized cells was also investigated for further application.

KW - 1,3-Propanediol

KW - Immobilized cell

KW - Inhibitory effect

KW - Klebsiella pneumoniae

KW - Waste glycerol

UR - http://www.scopus.com/inward/record.url?scp=85027940275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027940275&partnerID=8YFLogxK

U2 - 10.1016/j.cej.2016.12.029

DO - 10.1016/j.cej.2016.12.029

M3 - Article

AN - SCOPUS:85027940275

VL - 314

SP - 660

EP - 669

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

SN - 1385-8947

ER -