Repeated batch production of epothilone B by immobilized Sorangium cellulosum

Sang Woo Park, Su Jeong Park, Se Jong Han, Jinwoon Lee, Dong Shik Kim, Ji Heung Kim, Byung Woo Kim, Jeewon Lee, Sang Jun Sim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Production of extracellular epothilone B, one of the potent anticancer agents, by free and immobilized Sorangium cellulosum was studied using the repeated batch culture process. The concentration of alginate used in immobilization was directly related to the mass transfer rate of nutrients, mechanical stability, and the epothilone B production yield. With the optimized 3% (w/v) calcium alginate carrier, a prolonged repeated batch culture was investigated for the 5 repeated batches for 24 days. The maximum productivity of epothilone B obtained from the alginate-immobilized cells was 5.03 mg/l/day, which is 3 times higher than that of free cells (1.68 mg/l/day).

Original languageEnglish
Pages (from-to)1208-1212
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume17
Issue number7
Publication statusPublished - 2007 Jul 1

Fingerprint

Alginate
Batch Cell Culture Techniques
Immobilized Cells
Mechanical stability
Immobilization
Antineoplastic Agents
Nutrients
Calcium
Mass transfer
Productivity
Cells
Food
epothilone B
alginic acid

Keywords

  • Calcium alginate
  • Epothilone
  • Immobilization
  • Repeated batch culture
  • Sorangium cellulosum

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Park, S. W., Park, S. J., Han, S. J., Lee, J., Kim, D. S., Kim, J. H., ... Sim, S. J. (2007). Repeated batch production of epothilone B by immobilized Sorangium cellulosum. Journal of Microbiology and Biotechnology, 17(7), 1208-1212.

Repeated batch production of epothilone B by immobilized Sorangium cellulosum. / Park, Sang Woo; Park, Su Jeong; Han, Se Jong; Lee, Jinwoon; Kim, Dong Shik; Kim, Ji Heung; Kim, Byung Woo; Lee, Jeewon; Sim, Sang Jun.

In: Journal of Microbiology and Biotechnology, Vol. 17, No. 7, 01.07.2007, p. 1208-1212.

Research output: Contribution to journalArticle

Park, SW, Park, SJ, Han, SJ, Lee, J, Kim, DS, Kim, JH, Kim, BW, Lee, J & Sim, SJ 2007, 'Repeated batch production of epothilone B by immobilized Sorangium cellulosum', Journal of Microbiology and Biotechnology, vol. 17, no. 7, pp. 1208-1212.
Park SW, Park SJ, Han SJ, Lee J, Kim DS, Kim JH et al. Repeated batch production of epothilone B by immobilized Sorangium cellulosum. Journal of Microbiology and Biotechnology. 2007 Jul 1;17(7):1208-1212.
Park, Sang Woo ; Park, Su Jeong ; Han, Se Jong ; Lee, Jinwoon ; Kim, Dong Shik ; Kim, Ji Heung ; Kim, Byung Woo ; Lee, Jeewon ; Sim, Sang Jun. / Repeated batch production of epothilone B by immobilized Sorangium cellulosum. In: Journal of Microbiology and Biotechnology. 2007 ; Vol. 17, No. 7. pp. 1208-1212.
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