TY - JOUR
T1 - Repeated-batch production of omega-3 enriched biomass of Chlorella sorokiniana via calcium-induced homeoviscous adaptation
AU - Shim, Sung Jin
AU - Hong, Min Eui
AU - Chang, Won Seok
AU - Sim, Sang Jun
N1 - Funding Information:
This study was supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) (grant number: 20172010202050 ).
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/5
Y1 - 2020/5
N2 - This study aimed to improve valuable omega-3 fatty acids production in freshwater microalgae at normal temperature by inducing homeoviscous adaptation using CaCl2, which could have a role in decreasing the cellular membrane fluidity followed by increasing the rigidity of cell wall and membranes. At 10 mM CaCl2, simultaneous biomass and lipid production was obtained by Ca2+-based single strategy without considerable sacrifice of cellular logarithmic growth in Chlorella sorokiniana. The cells cultured at 10 mM CaCl2 (1-stage) showed relatively high levels of cellular membrane fluidity, caused by increased content in unsaturated fatty acids, compared to the conventional culture strategy (2-stage). Moreover, when this process was recycled by repeated-batch fermentation, the EPA productivity of 1-stage was 4.338 mg L−1 d−1, conspicuously increased by over 1300% compared to 2-stage. This strategy enhances the valuable omega-3 production, which can be commercially used for mass cultivation of omega-3-enriched biomass in the microalgae industry.
AB - This study aimed to improve valuable omega-3 fatty acids production in freshwater microalgae at normal temperature by inducing homeoviscous adaptation using CaCl2, which could have a role in decreasing the cellular membrane fluidity followed by increasing the rigidity of cell wall and membranes. At 10 mM CaCl2, simultaneous biomass and lipid production was obtained by Ca2+-based single strategy without considerable sacrifice of cellular logarithmic growth in Chlorella sorokiniana. The cells cultured at 10 mM CaCl2 (1-stage) showed relatively high levels of cellular membrane fluidity, caused by increased content in unsaturated fatty acids, compared to the conventional culture strategy (2-stage). Moreover, when this process was recycled by repeated-batch fermentation, the EPA productivity of 1-stage was 4.338 mg L−1 d−1, conspicuously increased by over 1300% compared to 2-stage. This strategy enhances the valuable omega-3 production, which can be commercially used for mass cultivation of omega-3-enriched biomass in the microalgae industry.
KW - Ca-based 1-stage recycling strategy
KW - Ca-induced homeoviscous adaptation
KW - Chlorella sorokiniana
KW - Eicosapentaenoic acid (EPA)
KW - Omega-3 fatty acids
UR - http://www.scopus.com/inward/record.url?scp=85079058250&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2020.122944
DO - 10.1016/j.biortech.2020.122944
M3 - Article
C2 - 32044645
AN - SCOPUS:85079058250
VL - 303
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
M1 - 122944
ER -