Repression of stress-responsive genes by FIERY2, a novel transcriptional regulator in Arabidopsis

Liming Xiong, Hojoung Lee, Manabu Ishitani, Yuko Tanaka, Becky Stevenson, Hisashi Koiwa, Ray A. Bressan, Paul M. Hasegawa, Jian Kang Zhu

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Low temperature, drought, and high salinity induce the expression of many plant genes. To understand the mechanisms for the transcriptional activation of these genes, we conducted a reporter gene-aided genetic screen in Arabidopsis. Seven allelic mutations in the FIERY2 (FRY2) locus result in significant increases in the expression of stress-responsive genes with the DRE/CRT (drought-responsive/C-repeat) cis element but non-DRE/CRT type stress-responsive genes were less affected. The specific regulation of DRE/CRT class of genes by FRY2 appears to be caused by repression of stress induction of the upstream CBF/DREB transcription factor genes. fry2 mutants show increased tolerance to salt stress and to abscisic acid during seed germination but are more sensitive to freezing damage at the seedling stage. FRY2/CPL1 encodes a novel transcriptional repressor harboring two double-stranded RNA-binding domains and a region homologous to the catalytic domain of RNA polymerase II C-terminal domain phosphatases found in yeast and in animals that regulate gene transcription. These data indicate that FRY2 is an important negative regulator of stress gene transcription and suggest that structured RNA may regulate hormone and stress responses in plants as it does in animals.

Original languageEnglish
Pages (from-to)10899-10904
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number16
DOIs
Publication statusPublished - 2002 Aug 1
Externally publishedYes

Fingerprint

Arabidopsis
Droughts
Genes
RNA Polymerase III
Plant Genes
Abscisic Acid
Catalytic RNA
RNA Polymerase II
Salinity
Regulator Genes
Germination
Seedlings
Reporter Genes
Phosphoric Monoester Hydrolases
Freezing
Transcriptional Activation
Catalytic Domain
Seeds
Transcription Factors
Salts

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Repression of stress-responsive genes by FIERY2, a novel transcriptional regulator in Arabidopsis. / Xiong, Liming; Lee, Hojoung; Ishitani, Manabu; Tanaka, Yuko; Stevenson, Becky; Koiwa, Hisashi; Bressan, Ray A.; Hasegawa, Paul M.; Zhu, Jian Kang.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 16, 01.08.2002, p. 10899-10904.

Research output: Contribution to journalArticle

Xiong, Liming ; Lee, Hojoung ; Ishitani, Manabu ; Tanaka, Yuko ; Stevenson, Becky ; Koiwa, Hisashi ; Bressan, Ray A. ; Hasegawa, Paul M. ; Zhu, Jian Kang. / Repression of stress-responsive genes by FIERY2, a novel transcriptional regulator in Arabidopsis. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 16. pp. 10899-10904.
@article{c18de91f891949c4ab1c38ad8cff7fb5,
title = "Repression of stress-responsive genes by FIERY2, a novel transcriptional regulator in Arabidopsis",
abstract = "Low temperature, drought, and high salinity induce the expression of many plant genes. To understand the mechanisms for the transcriptional activation of these genes, we conducted a reporter gene-aided genetic screen in Arabidopsis. Seven allelic mutations in the FIERY2 (FRY2) locus result in significant increases in the expression of stress-responsive genes with the DRE/CRT (drought-responsive/C-repeat) cis element but non-DRE/CRT type stress-responsive genes were less affected. The specific regulation of DRE/CRT class of genes by FRY2 appears to be caused by repression of stress induction of the upstream CBF/DREB transcription factor genes. fry2 mutants show increased tolerance to salt stress and to abscisic acid during seed germination but are more sensitive to freezing damage at the seedling stage. FRY2/CPL1 encodes a novel transcriptional repressor harboring two double-stranded RNA-binding domains and a region homologous to the catalytic domain of RNA polymerase II C-terminal domain phosphatases found in yeast and in animals that regulate gene transcription. These data indicate that FRY2 is an important negative regulator of stress gene transcription and suggest that structured RNA may regulate hormone and stress responses in plants as it does in animals.",
author = "Liming Xiong and Hojoung Lee and Manabu Ishitani and Yuko Tanaka and Becky Stevenson and Hisashi Koiwa and Bressan, {Ray A.} and Hasegawa, {Paul M.} and Zhu, {Jian Kang}",
year = "2002",
month = "8",
day = "1",
doi = "10.1073/pnas.162111599",
language = "English",
volume = "99",
pages = "10899--10904",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "16",

}

TY - JOUR

T1 - Repression of stress-responsive genes by FIERY2, a novel transcriptional regulator in Arabidopsis

AU - Xiong, Liming

AU - Lee, Hojoung

AU - Ishitani, Manabu

AU - Tanaka, Yuko

AU - Stevenson, Becky

AU - Koiwa, Hisashi

AU - Bressan, Ray A.

AU - Hasegawa, Paul M.

AU - Zhu, Jian Kang

PY - 2002/8/1

Y1 - 2002/8/1

N2 - Low temperature, drought, and high salinity induce the expression of many plant genes. To understand the mechanisms for the transcriptional activation of these genes, we conducted a reporter gene-aided genetic screen in Arabidopsis. Seven allelic mutations in the FIERY2 (FRY2) locus result in significant increases in the expression of stress-responsive genes with the DRE/CRT (drought-responsive/C-repeat) cis element but non-DRE/CRT type stress-responsive genes were less affected. The specific regulation of DRE/CRT class of genes by FRY2 appears to be caused by repression of stress induction of the upstream CBF/DREB transcription factor genes. fry2 mutants show increased tolerance to salt stress and to abscisic acid during seed germination but are more sensitive to freezing damage at the seedling stage. FRY2/CPL1 encodes a novel transcriptional repressor harboring two double-stranded RNA-binding domains and a region homologous to the catalytic domain of RNA polymerase II C-terminal domain phosphatases found in yeast and in animals that regulate gene transcription. These data indicate that FRY2 is an important negative regulator of stress gene transcription and suggest that structured RNA may regulate hormone and stress responses in plants as it does in animals.

AB - Low temperature, drought, and high salinity induce the expression of many plant genes. To understand the mechanisms for the transcriptional activation of these genes, we conducted a reporter gene-aided genetic screen in Arabidopsis. Seven allelic mutations in the FIERY2 (FRY2) locus result in significant increases in the expression of stress-responsive genes with the DRE/CRT (drought-responsive/C-repeat) cis element but non-DRE/CRT type stress-responsive genes were less affected. The specific regulation of DRE/CRT class of genes by FRY2 appears to be caused by repression of stress induction of the upstream CBF/DREB transcription factor genes. fry2 mutants show increased tolerance to salt stress and to abscisic acid during seed germination but are more sensitive to freezing damage at the seedling stage. FRY2/CPL1 encodes a novel transcriptional repressor harboring two double-stranded RNA-binding domains and a region homologous to the catalytic domain of RNA polymerase II C-terminal domain phosphatases found in yeast and in animals that regulate gene transcription. These data indicate that FRY2 is an important negative regulator of stress gene transcription and suggest that structured RNA may regulate hormone and stress responses in plants as it does in animals.

UR - http://www.scopus.com/inward/record.url?scp=0036677467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036677467&partnerID=8YFLogxK

U2 - 10.1073/pnas.162111599

DO - 10.1073/pnas.162111599

M3 - Article

C2 - 12149453

AN - SCOPUS:0036677467

VL - 99

SP - 10899

EP - 10904

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 16

ER -