TY - JOUR
T1 - Reproductive toxicity of folpet through deregulation of calcium homeostasis in porcine trophectoderm and luminal epithelial cells during early pregnancy
AU - Park, Sunwoo
AU - Yun, Bo Hyun
AU - Bae, Hyocheol
AU - Lim, Whasun
AU - Song, Gwonhwa
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIT) (No. 2021R1A2C2005841 , No. 2019R1A2C2089914 and No. 2021R1C1C1009807 ) and was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, South Korea (grant number: 2020R1I1A1A01067648 ).
Publisher Copyright:
© 2021
PY - 2021
Y1 - 2021
N2 - Folpet, a fungicide, is utilized even in cosmetics and pharmaceuticals. The LD50 of folpet in mammals, birds, and fish is relatively high. Recently, several negative effects of folpet on the respiratory system and cornea have been reported. However, there is no study on the negative effects of folpet on maternal–fetus interactions. In the present study, we used porcine trophectoderm (pTr) cells and porcine luminal epithelial (pLE) cells to investigate the toxic effects of folpet during implantation. Folpet treatment decreased cell proliferation and promoted apoptosis with cell cycle arrest. In addition, the ERK, JNK, and AKT signal pathways were activated by folpet treatment. Folpet treatment induced calcium overload in pTr and pLE cells mediating antimigratory and antiadhesive effects in both cell lines. Co-treatment with calcium chelates decreased the anti-implantation effect of folpet. Overall, our results demonstrated potential reproductive toxicity of folpet in pig.
AB - Folpet, a fungicide, is utilized even in cosmetics and pharmaceuticals. The LD50 of folpet in mammals, birds, and fish is relatively high. Recently, several negative effects of folpet on the respiratory system and cornea have been reported. However, there is no study on the negative effects of folpet on maternal–fetus interactions. In the present study, we used porcine trophectoderm (pTr) cells and porcine luminal epithelial (pLE) cells to investigate the toxic effects of folpet during implantation. Folpet treatment decreased cell proliferation and promoted apoptosis with cell cycle arrest. In addition, the ERK, JNK, and AKT signal pathways were activated by folpet treatment. Folpet treatment induced calcium overload in pTr and pLE cells mediating antimigratory and antiadhesive effects in both cell lines. Co-treatment with calcium chelates decreased the anti-implantation effect of folpet. Overall, our results demonstrated potential reproductive toxicity of folpet in pig.
KW - Calcium
KW - Folpet
KW - Luminal epithelial cell
KW - Migration
KW - Trophectoderm
UR - http://www.scopus.com/inward/record.url?scp=85116098262&partnerID=8YFLogxK
U2 - 10.1016/j.pestbp.2021.104974
DO - 10.1016/j.pestbp.2021.104974
M3 - Article
C2 - 34802524
AN - SCOPUS:85116098262
SN - 0048-3575
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
M1 - 104974
ER -