Resonant Rayleigh light scattering response of individual Au nanoparticles to antigen-antibody interaction

Cuong Cao, Sang Jun Sim

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A proof-of-concept study was reported on analysis of antigen-antibody recognition based on resonant Rayleigh scattering response of single Au nanoparticles in an imaging chamber. As benefited by a traditional dark-field microscope and a spectrograph, individual Au nanoparticles (30 nm) were observed with high signal-to-noise ratio and they were effectively utilized to monitor changes in refractive index induced by specific binding of the adsorbates. Using PSA antigen as a model, a LSPR λmax shift of about 2.85 nm was recorded for a molecular binding corresponding to 0.1 pg ml-1 of the protein biomarker. This result successfully demonstrates a non-labeling detection system for proteins as well as thousands of different chemical or biological species, and it possesses a great potential as a sensitive, on-chip and multiplexing detection.

Original languageEnglish
Pages (from-to)1836-1839
Number of pages4
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume9
Issue number13
DOIs
Publication statusPublished - 2009 Jul 13
Externally publishedYes

Fingerprint

Rayleigh scattering
Antigens
Antibodies
Light scattering
Nanoparticles
Proteins
Light
Refractometry
Spectrographs
Signal-To-Noise Ratio
Biomarkers
Adsorbates
Multiplexing
Refractive index
Signal to noise ratio
Microscopes
Imaging techniques

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

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