TY - JOUR
T1 - Response surface modeling of reductions in uropathogenic Escherichia coli biofilms on silicone by cranberry extract, caprylic acid, and thymol
AU - Kim, Hye Won
AU - Rhee, Min Suk
N1 - Funding Information:
This study was supported by the Korea Research Foundation (NRF-2016R1A2B2012743). The authors thank the School of Life Sciences and Biotechnology of Korea University for BK 21 PLUS, and the Institute of Biomedical Science and Food Safety, Korea University Food Safety Hall, for enabling access to their equipment and facilities. The authors are also grateful to Dr A.C. Matin of Stanford University School of Medicine for generously providing the clinical UPEC isolate used in this study.
PY - 2018/7/3
Y1 - 2018/7/3
N2 - A response surface methodology was used to build a model to predict reductions in uropathogenic Escherichia coli biofilms in response to three compounds: cranberry extract [CB] at 3.0–9.0%, and caprylic acid [CAR] and thymol [TM] at 0.01%–0.05%. The predictive model for microbial reduction had a high regression coefficient (R2 = 0.9988), and the accuracy of the model was verified (R2 = 0.9527). Values of CAR, TM, and the quadratic term CAR2 were the most significant (P < 0.0001) for bacterial reduction. Interactions between CB and CAR, and TM and CB, also affected bacterial reduction. The optimum conditions (a 5.8 log10 reduction) determined by ridge analysis were 8.3% CB +0.04% CAR +0.04% TM at 37 °C for 1 min. The model could be used to predict the most cost-efficient amounts of antimicrobial agents for anti-urinary tract infection products such as catheter lock solution and antimicrobial coatings for catheters.
AB - A response surface methodology was used to build a model to predict reductions in uropathogenic Escherichia coli biofilms in response to three compounds: cranberry extract [CB] at 3.0–9.0%, and caprylic acid [CAR] and thymol [TM] at 0.01%–0.05%. The predictive model for microbial reduction had a high regression coefficient (R2 = 0.9988), and the accuracy of the model was verified (R2 = 0.9527). Values of CAR, TM, and the quadratic term CAR2 were the most significant (P < 0.0001) for bacterial reduction. Interactions between CB and CAR, and TM and CB, also affected bacterial reduction. The optimum conditions (a 5.8 log10 reduction) determined by ridge analysis were 8.3% CB +0.04% CAR +0.04% TM at 37 °C for 1 min. The model could be used to predict the most cost-efficient amounts of antimicrobial agents for anti-urinary tract infection products such as catheter lock solution and antimicrobial coatings for catheters.
KW - Antimicrobial mixtures
KW - biofilm eradication; Escherichia coli
KW - natural products
KW - optimization modeling
KW - uropathogenic
UR - http://www.scopus.com/inward/record.url?scp=85053238062&partnerID=8YFLogxK
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U2 - 10.1080/08927014.2018.1488969
DO - 10.1080/08927014.2018.1488969
M3 - Article
C2 - 30187778
AN - SCOPUS:85053238062
VL - 34
SP - 710
EP - 717
JO - Biofouling
JF - Biofouling
SN - 0892-7014
IS - 6
ER -