Restoration of autophagy by puerarin in ethanol-treated hepatocytes via the activation of AMP-activated protein kinase

Byung Kyu Noh, Jung Kyu Lee, Hee jin Jun, Ji Hae Lee, Yaoyao Jia, Minh Hien Hoang, Jung Wan Kim, Kwan Hwa Park, Sung-Joon Lee

Research output: Contribution to journalArticle

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Abstract

We investigated the effects of puerarin, the major isoflavone in Kudzu roots, on the regulation of autophagy in ethanol-treated hepatocytes. Incubation in ethanol (100. mM) for 24. h reduced cell viability by 20% and increased the cellular concentrations of cholesterol and triglycerides by 40% and 20%, respectively. Puerarin stimulation significantly recovered cell viability and reduced cellular lipid accumulation to a level comparable to that in untreated control cells. Ethanol incubation reduced autophagy significantly as assessed by microtubule-associated protein1 light chain 3 (LC3) expression using immunohistochemistry and immunoblot analysis. The reduced expression of LC3 was restored by puerarin in a dose-dependent manner in ethanol-treated cells. The effect of puerarin on mammalian targets of rapamycin (mTOR), a key regulator of autophagy, was examined in ethanol-treated hepatocytes. Immunoblotting revealed that puerarin significantly induced the phosphorylation of 5'AMP-activated protein kinase (AMPK), thereby suppressing the mTOR target proteins S6 ribosomal protein and 4E-binding protein 1. These data suggest that puerarin restored the viability of cells and reduced lipid accumulation in ethanol-treated hepatocytes by activating autophagy via AMPK/mTOR-mediated signaling.

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume414
Issue number2
DOIs
Publication statusPublished - 2011 Oct 22

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AMP-Activated Protein Kinases
Autophagy
Restoration
Hepatocytes
Ethanol
Chemical activation
TOR Serine-Threonine Kinases
Cells
Cell Survival
Sirolimus
Pueraria
Ribosomal Protein S6
Lipids
S 6
Light
Phosphorylation
Isoflavones
Immunoblotting
Protein Binding
Microtubules

Keywords

  • Alcoholic liver damage
  • AMPK
  • Autophagy
  • MTOR
  • Puerarin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Restoration of autophagy by puerarin in ethanol-treated hepatocytes via the activation of AMP-activated protein kinase. / Noh, Byung Kyu; Lee, Jung Kyu; Jun, Hee jin; Lee, Ji Hae; Jia, Yaoyao; Hoang, Minh Hien; Kim, Jung Wan; Park, Kwan Hwa; Lee, Sung-Joon.

In: Biochemical and Biophysical Research Communications, Vol. 414, No. 2, 22.10.2011, p. 361-366.

Research output: Contribution to journalArticle

Noh, Byung Kyu ; Lee, Jung Kyu ; Jun, Hee jin ; Lee, Ji Hae ; Jia, Yaoyao ; Hoang, Minh Hien ; Kim, Jung Wan ; Park, Kwan Hwa ; Lee, Sung-Joon. / Restoration of autophagy by puerarin in ethanol-treated hepatocytes via the activation of AMP-activated protein kinase. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 414, No. 2. pp. 361-366.
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