Restoration of miR-29b exerts anti-cancer effects on glioblastoma

Jaekyung Shin, Hyun Geun Shim, Taeyoung Hwang, Hyungsin Kim, Shin Hyuk Kang, Yun Sik Dho, Sung Hye Park, Sang Jeong Kim, Chul Kee Park

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)

    Abstract

    Background: Glioblastoma multiforme (GBM) is known as one of the most fatal forms of cancer. MicroRNAs have been widely implicated in the regulation of mammalian development and pathogenesis. The brain-enriched miR-29 subfamilies are known to be exclusively expressed in the developing brain, and they are aberrantly down-regulated in GBM. This study aims to elucidate the role of miR-29b in GBM development and the feasibility of therapeutic targeting using conjugated nanoparticles. Methods: After confirmation of miR-29b expression levels in GBM tissues by analysis of open source data, the anticancer effect of miR-29b was tested by the introduction of syn-hsa-miR-29b-3p in the A172 GBM cell line. In vitro studies of cell viability and apoptosis and ex vivo study using GBM tissue slice cultures from 3 patients and nanoparticle delivery of miR-29b were performed. Results: We discovered an increase in apoptotic cell populations with the introduction of miR-29b in the GBM cell line. An established human-derived GBM tissue slice culture system confirmed the anticancer effect of miR-29b-conjugated nanoparticles. Using PCR array, we found that exogenous miR-29b inhibits the expression of COL1A2, COL3A1, COL4A1, ELN, ITGA11, MMP24, and SPARC, which mediates an anticancer effect. Conclusions: miR-29b may serve as a putative therapeutic molecule when its expression is restored using a nanoparticle delivery system in GBM.

    Original languageEnglish
    Article number104
    JournalCancer Cell International
    Volume17
    Issue number1
    DOIs
    Publication statusPublished - 2017 Nov 17

    Keywords

    • Anti-cancer effect
    • Glioblastoma
    • MiR-29b
    • Nanoparticle

    ASJC Scopus subject areas

    • Genetics
    • Oncology
    • Cancer Research

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