Retinal pigment epithelial cell behavior is modulated by alterations in focal cell-substrate contacts

Jung M. Lim, Sangwon Byun, Seok Chung, Tai Hyun Park, Jong M. Seo, Choun K. Joo, Hum Chung, Dong I. Cho

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

PURPOSE. To investigate how the cellular behavior of cultured retinal pigment epithelial (RPE) cells was affected by the manipulation of early focal contact. METHODS. To manipulate early focal contact, a reduced focal cell-substrate contact area on the micropatterned surfaces was implemented by microfabrication with polydimethylsiloxane (PDMS). The micropatterned PDMS surfaces had a circular pillar with a diameter of 5 μm. The human retinal pigment epithelial cell line, ARPE-19, was seeded onto the fibronectin-coated PDMS surfaces. Cell adhesion, growth, cell cycle, morphology, and interleukin-6 (IL-6) expression were observed, for 3 weeks. RESULTS. The fluorescent images of localized vinculin. and actin stress fibers appeared to be more prominent on smooth PDMS surfaces. Although there was no significant effect on cell adhesion, a statistically significant inhibition of cell cycle progression was observed for micropatterned PDMS surfaces. Similarly, micropatterned surfaces showed significantly less cell growth than that of smooth surfaces. Cultures over a period of 3 weeks showed a distinct cell-cell phenotype discrepancy. Furthermore, IL-6 mRNA and secreted protein induced by IL-1β in ARPE-19 were downregulated on micropatterned PDMS surfaces. CONCLUSIONS. Disturbed focal contact in ARPE-19 cells grown on micropatterned surfaces altered cell cycle, growth, morphology, and the expression of IL-6 in vitro.

Original languageEnglish
Pages (from-to)4210-4216
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume45
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1
Externally publishedYes

Fingerprint

Retinal Pigments
Epithelial Cells
Focal Adhesions
Interleukin-6
Cell Cycle
Cell Adhesion
Growth
Microtechnology
Vinculin
Stress Fibers
Interleukin-1
Fibronectins
baysilon
Actins
Down-Regulation
Phenotype
Cell Line
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Retinal pigment epithelial cell behavior is modulated by alterations in focal cell-substrate contacts. / Lim, Jung M.; Byun, Sangwon; Chung, Seok; Park, Tai Hyun; Seo, Jong M.; Joo, Choun K.; Chung, Hum; Cho, Dong I.

In: Investigative Ophthalmology and Visual Science, Vol. 45, No. 11, 01.11.2004, p. 4210-4216.

Research output: Contribution to journalArticle

Lim, Jung M. ; Byun, Sangwon ; Chung, Seok ; Park, Tai Hyun ; Seo, Jong M. ; Joo, Choun K. ; Chung, Hum ; Cho, Dong I. / Retinal pigment epithelial cell behavior is modulated by alterations in focal cell-substrate contacts. In: Investigative Ophthalmology and Visual Science. 2004 ; Vol. 45, No. 11. pp. 4210-4216.
@article{4620cbbeb83f428b88f1a5a3d7458ee0,
title = "Retinal pigment epithelial cell behavior is modulated by alterations in focal cell-substrate contacts",
abstract = "PURPOSE. To investigate how the cellular behavior of cultured retinal pigment epithelial (RPE) cells was affected by the manipulation of early focal contact. METHODS. To manipulate early focal contact, a reduced focal cell-substrate contact area on the micropatterned surfaces was implemented by microfabrication with polydimethylsiloxane (PDMS). The micropatterned PDMS surfaces had a circular pillar with a diameter of 5 μm. The human retinal pigment epithelial cell line, ARPE-19, was seeded onto the fibronectin-coated PDMS surfaces. Cell adhesion, growth, cell cycle, morphology, and interleukin-6 (IL-6) expression were observed, for 3 weeks. RESULTS. The fluorescent images of localized vinculin. and actin stress fibers appeared to be more prominent on smooth PDMS surfaces. Although there was no significant effect on cell adhesion, a statistically significant inhibition of cell cycle progression was observed for micropatterned PDMS surfaces. Similarly, micropatterned surfaces showed significantly less cell growth than that of smooth surfaces. Cultures over a period of 3 weeks showed a distinct cell-cell phenotype discrepancy. Furthermore, IL-6 mRNA and secreted protein induced by IL-1β in ARPE-19 were downregulated on micropatterned PDMS surfaces. CONCLUSIONS. Disturbed focal contact in ARPE-19 cells grown on micropatterned surfaces altered cell cycle, growth, morphology, and the expression of IL-6 in vitro.",
author = "Lim, {Jung M.} and Sangwon Byun and Seok Chung and Park, {Tai Hyun} and Seo, {Jong M.} and Joo, {Choun K.} and Hum Chung and Cho, {Dong I.}",
year = "2004",
month = "11",
day = "1",
doi = "10.1167/iovs.03-1036",
language = "English",
volume = "45",
pages = "4210--4216",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "11",

}

TY - JOUR

T1 - Retinal pigment epithelial cell behavior is modulated by alterations in focal cell-substrate contacts

AU - Lim, Jung M.

AU - Byun, Sangwon

AU - Chung, Seok

AU - Park, Tai Hyun

AU - Seo, Jong M.

AU - Joo, Choun K.

AU - Chung, Hum

AU - Cho, Dong I.

PY - 2004/11/1

Y1 - 2004/11/1

N2 - PURPOSE. To investigate how the cellular behavior of cultured retinal pigment epithelial (RPE) cells was affected by the manipulation of early focal contact. METHODS. To manipulate early focal contact, a reduced focal cell-substrate contact area on the micropatterned surfaces was implemented by microfabrication with polydimethylsiloxane (PDMS). The micropatterned PDMS surfaces had a circular pillar with a diameter of 5 μm. The human retinal pigment epithelial cell line, ARPE-19, was seeded onto the fibronectin-coated PDMS surfaces. Cell adhesion, growth, cell cycle, morphology, and interleukin-6 (IL-6) expression were observed, for 3 weeks. RESULTS. The fluorescent images of localized vinculin. and actin stress fibers appeared to be more prominent on smooth PDMS surfaces. Although there was no significant effect on cell adhesion, a statistically significant inhibition of cell cycle progression was observed for micropatterned PDMS surfaces. Similarly, micropatterned surfaces showed significantly less cell growth than that of smooth surfaces. Cultures over a period of 3 weeks showed a distinct cell-cell phenotype discrepancy. Furthermore, IL-6 mRNA and secreted protein induced by IL-1β in ARPE-19 were downregulated on micropatterned PDMS surfaces. CONCLUSIONS. Disturbed focal contact in ARPE-19 cells grown on micropatterned surfaces altered cell cycle, growth, morphology, and the expression of IL-6 in vitro.

AB - PURPOSE. To investigate how the cellular behavior of cultured retinal pigment epithelial (RPE) cells was affected by the manipulation of early focal contact. METHODS. To manipulate early focal contact, a reduced focal cell-substrate contact area on the micropatterned surfaces was implemented by microfabrication with polydimethylsiloxane (PDMS). The micropatterned PDMS surfaces had a circular pillar with a diameter of 5 μm. The human retinal pigment epithelial cell line, ARPE-19, was seeded onto the fibronectin-coated PDMS surfaces. Cell adhesion, growth, cell cycle, morphology, and interleukin-6 (IL-6) expression were observed, for 3 weeks. RESULTS. The fluorescent images of localized vinculin. and actin stress fibers appeared to be more prominent on smooth PDMS surfaces. Although there was no significant effect on cell adhesion, a statistically significant inhibition of cell cycle progression was observed for micropatterned PDMS surfaces. Similarly, micropatterned surfaces showed significantly less cell growth than that of smooth surfaces. Cultures over a period of 3 weeks showed a distinct cell-cell phenotype discrepancy. Furthermore, IL-6 mRNA and secreted protein induced by IL-1β in ARPE-19 were downregulated on micropatterned PDMS surfaces. CONCLUSIONS. Disturbed focal contact in ARPE-19 cells grown on micropatterned surfaces altered cell cycle, growth, morphology, and the expression of IL-6 in vitro.

UR - http://www.scopus.com/inward/record.url?scp=6944242613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6944242613&partnerID=8YFLogxK

U2 - 10.1167/iovs.03-1036

DO - 10.1167/iovs.03-1036

M3 - Article

VL - 45

SP - 4210

EP - 4216

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 11

ER -