Retinal pigment epithelial cell behavior is modulated by alterations in focal cell-substrate contacts

Jung Min Lim, Sangwon Byun, Seok Chung, Tai Hyun Park, Jong Mo Seo, Choun Ki Joo, Hum Chung, Dong Il Cho

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

PURPOSE. To investigate how the cellular behavior of cultured retinal pigment epithelial (RPE) cells was affected by the manipulation of early focal contact. METHODS. To manipulate early focal contact, a reduced focal cell-substrate contact area on the micropatterned surfaces was implemented by microfabrication with polydimethylsiloxane (PDMS). The micropatterned PDMS surfaces had a circular pillar with a diameter of 5 μm. The human retinal pigment epithelial cell line, ARPE-19, was seeded onto the fibronectin-coated PDMS surfaces. Cell adhesion, growth, cell cycle, morphology, and interleukin-6 (IL-6) expression were observed, for 3 weeks. RESULTS. The fluorescent images of localized vinculin. and actin stress fibers appeared to be more prominent on smooth PDMS surfaces. Although there was no significant effect on cell adhesion, a statistically significant inhibition of cell cycle progression was observed for micropatterned PDMS surfaces. Similarly, micropatterned surfaces showed significantly less cell growth than that of smooth surfaces. Cultures over a period of 3 weeks showed a distinct cell-cell phenotype discrepancy. Furthermore, IL-6 mRNA and secreted protein induced by IL-1β in ARPE-19 were downregulated on micropatterned PDMS surfaces. CONCLUSIONS. Disturbed focal contact in ARPE-19 cells grown on micropatterned surfaces altered cell cycle, growth, morphology, and the expression of IL-6 in vitro.

Original languageEnglish
Pages (from-to)4210-4216
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume45
Issue number11
DOIs
Publication statusPublished - 2004 Nov

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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