@article{f31c79bdbb714484b5a2c0cfaa208ba2,
title = "Retraction fibers produced by fibronectin-integrin α5β1 interaction promote motility of brain tumor cells",
abstract = "Glioblastoma (GBM) is a refractory disease that has a highly infiltrative characteristic. Over the past decade, GBM perivascular niche (PVN) has been described as a route of dissemination. Here, we investigated that trailed membrane structures, namely retraction fibers (RFs), are formed by perivascular extracellular matrix (ECM) proteins. By using the anatomical GBM database, we validated that the ECM-related genes were highly expressed in the cells within the PVN where fibronectin (FN) induced RF formation. By disrupting candidates of FN-binding integrins, integrin α5β1 was identified as the main regulator of RF formation. De novo RFs were produced at the trailing edge, and focal adhesions were actively localized in RFs, indicating that adhesive force makes RFs remain at the bottom surface. Furthermore, we observed that GBM cells more frequently migrated along the residual RFs formed by preceding cells in microfluidic channels in comparison to those in the channels without RFs, suggesting that the infiltrative characteristics GBM could be attributed to RFs formed by the preceding cells in concert with chemoattractant cues. Altogether, we demonstrated that shedding membrane structures of GBM cells are maintained by FN-integrin α5β1 interaction and promoted their motility.",
keywords = "cell migration, fibronectin, integrin α5β1, perivascular niche, retraction fiber",
author = "Lee, {Seon Yong} and Choi, {Sang Hun} and Lee, {Min Seok} and Amanzhol Kurmashev and Lee, {Hae Nim} and Ko, {Young Gyu} and Kanghun Lee and Sohee Jeong and Jihye Seong and Kang, {Joo H.} and Hyunggee Kim",
note = "Funding Information: We are grateful to all members of the Cancer Growth Regulation Laboratory for their helpful discussion and technical assistance. We thank the staff of Gyerim Experimental Animal Resource Center (GEARC) for animal care and technical assistance. We also would like to thank J. ‐H. Hur and H. ‐C. Joung of UNIST Optical Biomed‐Imaging Center (UOBC) for technical advice. This study was supported by grants to H. Kim from the National Research Foundation of Korea (NRF) (2015R1A5A1009024), and from the NRF (2019R1C1C1006124) to J. H. Kang, and from KIST institutional grant (2E30963) to J. Seong, and the School of Life Sciences and Biotechnology for BK21, Korea University. Funding Information: We are grateful to all members of the Cancer Growth Regulation Laboratory for their helpful discussion and technical assistance. We thank the staff of Gyerim Experimental Animal Resource Center (GEARC) for animal care and technical assistance. We also would like to thank J. -H. Hur and H. -C. Joung of UNIST Optical Biomed-Imaging Center (UOBC) for technical advice. This study was supported by grants to H. Kim from the National Research Foundation of Korea (NRF) (2015R1A5A1009024), and from the NRF (2019R1C1C1006124) to J. H. Kang, and from KIST institutional grant (2E30963) to J. Seong, and the School of Life Sciences and Biotechnology for BK21, Korea University. Publisher Copyright: {\textcopyright} 2021 Federation of American Societies for Experimental Biology.",
year = "2021",
month = oct,
doi = "10.1096/fj.202100452RR",
language = "English",
volume = "35",
journal = "The FASEB journal : official publication of the Federation of American Societies for Experimental Biology",
issn = "1530-6860",
publisher = "FASEB",
number = "10",
}