Reversible and multi-cyclic protein-protein interaction in bacterial cellulosome-mimic system using rod-shaped viral nanostructure

Hyun Jin Kim, Eun Jung Lee, Jin Seung Park, Sang Jun Sim, Jeewon Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The type II cohesin domain and type II dockerin of bacterial cellulosome were cloned from Clostridium thermocellum and expressed with the fusion of tobacco mosaic virus coat protein (TMVcp) and enhanced green fluorescent protein (EGFP), respectively, in Escherichia coli. The TMVcp-cohesin fusion protein was assembled to the stable and rod-shaped nanostructure (TMVcp-Coh rod) under a particular buffer condition, where many active cohesin proteins are biologically and densely displayed around the 3-dimensional surface of TMVcp-Coh rod. Using EGFP-dockerin as a fluorescent reporter, we confirmed that the Ca2+-dependent binding and dissociation between native cohesin and dockerin were reproduced with the two recombinant fusion proteins, TMVcp-cohesin and EGFP-dockerin. The multi-cyclic binding-dissociation operation of TMVcp-Coh rod and EGFP-dockerin was successfully performed with maintaining the reversible cohesin-dockerin interaction in every cycle. EGFP that was fused to dockerin as a proof-of-concept here can be switched to other functional proteins/peptides that need to be used in multi-cyclic operation.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalJournal of Biotechnology
Volume221
DOIs
Publication statusPublished - 2016 Mar 10

Fingerprint

Cellulosomes
Tobacco Mosaic Virus
Nanostructures
Capsid Proteins
Proteins
Clostridium thermocellum
Recombinant Fusion Proteins
cohesins
Buffers
enhanced green fluorescent protein
Escherichia coli
Peptides

Keywords

  • Cohesin
  • Dockerin
  • Multi-cyclic operation
  • Reversible interaction
  • Tobacco mosaic virus coat protein

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Reversible and multi-cyclic protein-protein interaction in bacterial cellulosome-mimic system using rod-shaped viral nanostructure. / Kim, Hyun Jin; Lee, Eun Jung; Park, Jin Seung; Sim, Sang Jun; Lee, Jeewon.

In: Journal of Biotechnology, Vol. 221, 10.03.2016, p. 101-106.

Research output: Contribution to journalArticle

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