RGD direct conjugation using co-solvent system to increase cell attachment on poly(lactide-co-glycolide) without change in hydrophilic/hydrophobic property

Soo Hong Lee, Hyung Min Chung, Young Ha Kim, Soo Hyun Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have developed a direct conjugation method of ROD (Arg-Gly-Asp) into polylactide (PLA) using co-solvent system, which showed high reaction yield compared to one solvent system presumably due to good solubility of reactants. First, hydroxyl groups at the end of PLA were changed into carboxyl acid by ring opening reaction of succinic anhydride. For a coupling reaction with high solubility, ROD was dissolved in water and then PLA in dioxane was added into ROD solution in the presence of 1-(3-dimenthylaminopropyl)3-ethylcarbodiimide hydrochloride (EDC). The conjugation of ROD was confirmed by 1H-NMR, GPC and elementary analysis. The ROD conjugated PLA (RGD-PLA) on poly(lactide-co-glycolide) (PLGA) film significantly increased the number of attached cells compared to PLGA without RGD-PLA, while it did not change static contact angle of PLGA. This result demonstrated this conjugation method would be a useful tool to increase cell affinity of biomaterials without change in hydrophilic/hydrophobic property for tissue engineering applications.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalKey Engineering Materials
Volume342-343
Publication statusPublished - 2007 Apr 16
Externally publishedYes

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Keywords

  • Cell adhesion
  • Hydrophilic/hydrophobic property
  • Poly(lactide-co-glycolide)
  • Polylactide
  • RGD conjugation
  • Tissue engineering

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemical Engineering (miscellaneous)

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