Rheological characteristics of erythrocytes incubated in glucose media

Sehyun Shin, Yun Hee Ku, Jang Soo Suh, M. Singh

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Glucose-rich plasma is commonly observed in diabetes mellitus and in-vitro incubation of erythrocytes in glucose-rich media may produce the non-enzymatic glycosylation of erythrocyte proteins. The present study investigates the effects of an increased concentration of glucose in a suspending medium on erythrocyte rheological parameters. Erythrocytes, which were obtained from ten healthy volunteers, were washed and incubated in vitro with glucose solutions at different concentrations and incubation times. The measured hemorheological parameters included deformability and the aggregation of erythrocytes. Hemoglobin concentration in the erythrocyte suspension in autologous plasma was also measured after incubation in order to assess the hemolysis of erythrocytes. Significant hemorheological changes were observed with an increase of glucose concentration and incubating time. Both deformability and the aggregation of erythrocytes decreased in dose- and time-dependent manner. A reduction in hemoglobin concentration in the erythrocyte suspensions was also observed due to hemolysis of fragile erythrocytes during incubation. Modification in the hemorheological properties of cells, which may be associated with due to glucose-induced (auto)oxidation and glycation of erythrocyte proteins in hyperglycemia, can adversely affect the fluidity and oxygen-delivery function of the erythrocytes.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalClinical Hemorheology and Microcirculation
Volume38
Issue number3
Publication statusPublished - 2008 Feb 6

Fingerprint

Erythrocytes
Glucose
Erythrocyte Aggregation
Erythrocyte Deformability
Hemolysis
Suspensions
Hemoglobins
Glycosylation
Hyperglycemia
Diabetes Mellitus
Healthy Volunteers
Oxygen
Proteins

Keywords

  • Aggregation
  • Deformability
  • Erythrocytes
  • Glucose
  • Glycation

ASJC Scopus subject areas

  • Hematology
  • Physiology

Cite this

Rheological characteristics of erythrocytes incubated in glucose media. / Shin, Sehyun; Ku, Yun Hee; Suh, Jang Soo; Singh, M.

In: Clinical Hemorheology and Microcirculation, Vol. 38, No. 3, 06.02.2008, p. 153-161.

Research output: Contribution to journalArticle

Shin, Sehyun ; Ku, Yun Hee ; Suh, Jang Soo ; Singh, M. / Rheological characteristics of erythrocytes incubated in glucose media. In: Clinical Hemorheology and Microcirculation. 2008 ; Vol. 38, No. 3. pp. 153-161.
@article{98f240b9431d4c22869a50cbec562fcd,
title = "Rheological characteristics of erythrocytes incubated in glucose media",
abstract = "Glucose-rich plasma is commonly observed in diabetes mellitus and in-vitro incubation of erythrocytes in glucose-rich media may produce the non-enzymatic glycosylation of erythrocyte proteins. The present study investigates the effects of an increased concentration of glucose in a suspending medium on erythrocyte rheological parameters. Erythrocytes, which were obtained from ten healthy volunteers, were washed and incubated in vitro with glucose solutions at different concentrations and incubation times. The measured hemorheological parameters included deformability and the aggregation of erythrocytes. Hemoglobin concentration in the erythrocyte suspension in autologous plasma was also measured after incubation in order to assess the hemolysis of erythrocytes. Significant hemorheological changes were observed with an increase of glucose concentration and incubating time. Both deformability and the aggregation of erythrocytes decreased in dose- and time-dependent manner. A reduction in hemoglobin concentration in the erythrocyte suspensions was also observed due to hemolysis of fragile erythrocytes during incubation. Modification in the hemorheological properties of cells, which may be associated with due to glucose-induced (auto)oxidation and glycation of erythrocyte proteins in hyperglycemia, can adversely affect the fluidity and oxygen-delivery function of the erythrocytes.",
keywords = "Aggregation, Deformability, Erythrocytes, Glucose, Glycation",
author = "Sehyun Shin and Ku, {Yun Hee} and Suh, {Jang Soo} and M. Singh",
year = "2008",
month = "2",
day = "6",
language = "English",
volume = "38",
pages = "153--161",
journal = "Clinical Hemorheology and Microcirculation",
issn = "1386-0291",
publisher = "IOS Press",
number = "3",

}

TY - JOUR

T1 - Rheological characteristics of erythrocytes incubated in glucose media

AU - Shin, Sehyun

AU - Ku, Yun Hee

AU - Suh, Jang Soo

AU - Singh, M.

PY - 2008/2/6

Y1 - 2008/2/6

N2 - Glucose-rich plasma is commonly observed in diabetes mellitus and in-vitro incubation of erythrocytes in glucose-rich media may produce the non-enzymatic glycosylation of erythrocyte proteins. The present study investigates the effects of an increased concentration of glucose in a suspending medium on erythrocyte rheological parameters. Erythrocytes, which were obtained from ten healthy volunteers, were washed and incubated in vitro with glucose solutions at different concentrations and incubation times. The measured hemorheological parameters included deformability and the aggregation of erythrocytes. Hemoglobin concentration in the erythrocyte suspension in autologous plasma was also measured after incubation in order to assess the hemolysis of erythrocytes. Significant hemorheological changes were observed with an increase of glucose concentration and incubating time. Both deformability and the aggregation of erythrocytes decreased in dose- and time-dependent manner. A reduction in hemoglobin concentration in the erythrocyte suspensions was also observed due to hemolysis of fragile erythrocytes during incubation. Modification in the hemorheological properties of cells, which may be associated with due to glucose-induced (auto)oxidation and glycation of erythrocyte proteins in hyperglycemia, can adversely affect the fluidity and oxygen-delivery function of the erythrocytes.

AB - Glucose-rich plasma is commonly observed in diabetes mellitus and in-vitro incubation of erythrocytes in glucose-rich media may produce the non-enzymatic glycosylation of erythrocyte proteins. The present study investigates the effects of an increased concentration of glucose in a suspending medium on erythrocyte rheological parameters. Erythrocytes, which were obtained from ten healthy volunteers, were washed and incubated in vitro with glucose solutions at different concentrations and incubation times. The measured hemorheological parameters included deformability and the aggregation of erythrocytes. Hemoglobin concentration in the erythrocyte suspension in autologous plasma was also measured after incubation in order to assess the hemolysis of erythrocytes. Significant hemorheological changes were observed with an increase of glucose concentration and incubating time. Both deformability and the aggregation of erythrocytes decreased in dose- and time-dependent manner. A reduction in hemoglobin concentration in the erythrocyte suspensions was also observed due to hemolysis of fragile erythrocytes during incubation. Modification in the hemorheological properties of cells, which may be associated with due to glucose-induced (auto)oxidation and glycation of erythrocyte proteins in hyperglycemia, can adversely affect the fluidity and oxygen-delivery function of the erythrocytes.

KW - Aggregation

KW - Deformability

KW - Erythrocytes

KW - Glucose

KW - Glycation

UR - http://www.scopus.com/inward/record.url?scp=38749106232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38749106232&partnerID=8YFLogxK

M3 - Article

C2 - 18239257

AN - SCOPUS:38749106232

VL - 38

SP - 153

EP - 161

JO - Clinical Hemorheology and Microcirculation

JF - Clinical Hemorheology and Microcirculation

SN - 1386-0291

IS - 3

ER -