TY - JOUR
T1 - Rhizorhabdus phycosphaerae sp. nov., isolated from the phycosphere of Microcystis aeruginosa
AU - Kim, Minkyung
AU - Bae, Junshik
AU - Park, Woojun
N1 - Funding Information:
This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002108).
Publisher Copyright:
© 2022 The Authors.
PY - 2022
Y1 - 2022
N2 - A novel bacterial strain, designated MK52T, was isolated from the phycosphere of Microcystis aeruginosa. Strain MK52T is a Gram-stain-negative, pink-pigmented, rod-shaped, strictly aerobic bacterium. In 16S rRNA phylogenetic analysis, the MK52T strain was most closely related to Rhizorhabdus wittichii RW1T (98.66%) and Rhizorhabdus histidinilytica UM2T (98.51%). The genomic DNA G+C content of strain MK52T was calculated to be 65.5mol%. The average nucleotide identity values of strain MK52T with R. wittichii RW1T and R. histidinilytica UM2T were 80.35 and 80.23%, respectively, with digital DNA–DNA hybridization values of 23.6 and 22.9%, respectively, and average amino acid identities of 75.59 and 75.79%, respectively. The major isopre-noid quinone was Q-10, and the predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyle-thanolamine and sphingoglycolipid. Fatty acid methyl ether analysis showed that summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) was the main cellular fatty acid in strain MK52T. Strain MK52T cells grew at 21–34 °C (optimum, 30 °C), pH 5–8 (optimum, pH 7) and with 0–2% (w/v) NaCl (optimum, 0.5% NaCl). Rhizorhabdus phycosphaerae sp. nov. is proposed as a new species (=KCTC 72877T=DSM 111424T) based on its genotypic and phenotypic characteristics.
AB - A novel bacterial strain, designated MK52T, was isolated from the phycosphere of Microcystis aeruginosa. Strain MK52T is a Gram-stain-negative, pink-pigmented, rod-shaped, strictly aerobic bacterium. In 16S rRNA phylogenetic analysis, the MK52T strain was most closely related to Rhizorhabdus wittichii RW1T (98.66%) and Rhizorhabdus histidinilytica UM2T (98.51%). The genomic DNA G+C content of strain MK52T was calculated to be 65.5mol%. The average nucleotide identity values of strain MK52T with R. wittichii RW1T and R. histidinilytica UM2T were 80.35 and 80.23%, respectively, with digital DNA–DNA hybridization values of 23.6 and 22.9%, respectively, and average amino acid identities of 75.59 and 75.79%, respectively. The major isopre-noid quinone was Q-10, and the predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyle-thanolamine and sphingoglycolipid. Fatty acid methyl ether analysis showed that summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) was the main cellular fatty acid in strain MK52T. Strain MK52T cells grew at 21–34 °C (optimum, 30 °C), pH 5–8 (optimum, pH 7) and with 0–2% (w/v) NaCl (optimum, 0.5% NaCl). Rhizorhabdus phycosphaerae sp. nov. is proposed as a new species (=KCTC 72877T=DSM 111424T) based on its genotypic and phenotypic characteristics.
KW - Alphaproteobacteria
KW - cyanobacteria
KW - freshwater
KW - Microcystis aeruginosa
KW - Rhizorhabdus
KW - sphingolipid
UR - http://www.scopus.com/inward/record.url?scp=85128673630&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.005324
DO - 10.1099/ijsem.0.005324
M3 - Article
C2 - 35446755
AN - SCOPUS:85128673630
SN - 1466-5026
VL - 72
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 4
M1 - 005324
ER -