Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling

Ji Won Park, Ok Kyoung Kwon, Jung Hee Kim, Sei Ryang Oh, Jae-Hong Kim, Jin Hyub Paik, Bambang Marwoto, Rifatul Widjhati, Fifit Juniarti, Doddy Irawan, Kyung Seop Ahn

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rhododendron album Blume (RA) has traditionally been used as an herbal medicine and is considered to have anti-inflammatory properties. In the present study, we screened RA extracts with anti-inflammatory properties. The biological effects of an RA methanol extract (RAME) on inflammation were investigated in lipopolysaccharide (LPS)-stimulated mouse RAW264.7 cells. We investigated the effects of RAME on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW264.7 cells. To explore the anti-inflammatory mechanisms of RAME, we measured the mRNA and protein expression of pro-inflammatory mediators induced by RAME in the LPS-stimulated RAW264.7 cells by RT-PCR and western blot analysis, respectively. RAME significantly inhibited the production of NO, PGE2, interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α in the LPS-stimulated RAW264.7 cells. It also suppressed the mRNA and protein expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and mitogen-activated protein kinases (MAPKs) with a concomitant decrease in the nuclear translocation of nuclear factor-κB (NF-κB) in the LPS-stimulated RAW264.7 cells. These results indicate that RAME inhibits LPS-induced inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-κB activation. We therefore suggest that RAME may be prove to be an effective therapeutic agent for the treatment of inflammatory diseases.

Original languageEnglish
Pages (from-to)987-994
Number of pages8
JournalInternational Journal of Molecular Medicine
Volume35
Issue number4
DOIs
Publication statusPublished - 2015 Apr 1

Fingerprint

Rhododendron
Cyclooxygenase 2
Nitric Oxide Synthase
Methanol
Lipopolysaccharides
Down-Regulation
Anti-Inflammatory Agents
Dinoprostone
Nitric Oxide
Messenger RNA
Herbal Medicine
Nitric Oxide Synthase Type II
Mitogen-Activated Protein Kinases
Interleukin-1
Interleukin-6
Proteins
Tumor Necrosis Factor-alpha
Western Blotting
Inflammation
Polymerase Chain Reaction

Keywords

  • Inflammation
  • Lipopolysaccharide
  • Mitogen-activated protein kinases
  • Nuclear factor-κB
  • Rhododendron album Blume

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling. / Park, Ji Won; Kwon, Ok Kyoung; Kim, Jung Hee; Oh, Sei Ryang; Kim, Jae-Hong; Paik, Jin Hyub; Marwoto, Bambang; Widjhati, Rifatul; Juniarti, Fifit; Irawan, Doddy; Ahn, Kyung Seop.

In: International Journal of Molecular Medicine, Vol. 35, No. 4, 01.04.2015, p. 987-994.

Research output: Contribution to journalArticle

Park, Ji Won ; Kwon, Ok Kyoung ; Kim, Jung Hee ; Oh, Sei Ryang ; Kim, Jae-Hong ; Paik, Jin Hyub ; Marwoto, Bambang ; Widjhati, Rifatul ; Juniarti, Fifit ; Irawan, Doddy ; Ahn, Kyung Seop. / Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling. In: International Journal of Molecular Medicine. 2015 ; Vol. 35, No. 4. pp. 987-994.
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AU - Kim, Jae-Hong

AU - Paik, Jin Hyub

AU - Marwoto, Bambang

AU - Widjhati, Rifatul

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AU - Irawan, Doddy

AU - Ahn, Kyung Seop

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AB - Rhododendron album Blume (RA) has traditionally been used as an herbal medicine and is considered to have anti-inflammatory properties. In the present study, we screened RA extracts with anti-inflammatory properties. The biological effects of an RA methanol extract (RAME) on inflammation were investigated in lipopolysaccharide (LPS)-stimulated mouse RAW264.7 cells. We investigated the effects of RAME on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW264.7 cells. To explore the anti-inflammatory mechanisms of RAME, we measured the mRNA and protein expression of pro-inflammatory mediators induced by RAME in the LPS-stimulated RAW264.7 cells by RT-PCR and western blot analysis, respectively. RAME significantly inhibited the production of NO, PGE2, interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α in the LPS-stimulated RAW264.7 cells. It also suppressed the mRNA and protein expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and mitogen-activated protein kinases (MAPKs) with a concomitant decrease in the nuclear translocation of nuclear factor-κB (NF-κB) in the LPS-stimulated RAW264.7 cells. These results indicate that RAME inhibits LPS-induced inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-κB activation. We therefore suggest that RAME may be prove to be an effective therapeutic agent for the treatment of inflammatory diseases.

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