Rhus verniciflua extract modulates survival of MCF-7 breast cancer cells through the modulation of AMPK-pathway

Jung Ok Lee, Ji Wook Moon, Soo Kyung Lee, So Mi Kim, Nami Kim, Seong Gyu Ko, Hyeon Soo Kim, Sun Hwa Park

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Rhus verniciflua STOKES (RVS) is used as an anti-cancer agent in traditional herbal medicine. However, the underlying molecular mechanism of its action is poorly understood. Here, we elucidated the mechanism of the anti-cancer mechanism of RVS in MCF-7 human breast cancer cells. We found that RVS increased the phosphorylation of AMP-activated protein kinase (AMPK) and downstream acetyl-CoA carboxylase (ACC) and suppressed cell viability in an AMPK-dependent fashion. RVS also induced an increase in reactive oxygen species (ROS) levels. RVS-induced AMPK phosphorylation was not observed in the presence of N -acetyl-cysteine (NAC), which indicated that ROS is associated with RVS-induced AMPK phosphorylation. In addition, fluorescent staining (Annexin V/propidium iodide) revealed that RVS increased the expression of Annexin V, which indicates that RVS leads to cancer-induced apoptosis. Moreover, RVS increased the phosphorylation of p53 and the expression of Bax. The inhibition of AMPK blocked RVS-induced p53 phosphorylation and Bax expression, which suggests that AMPK is involved in RVS-induced cancer apoptosis. Taken together, these results demonstrate that RVS has anti-tumor effects on MCF-7 cells through an AMPK-signaling pathway.

Original languageEnglish
Pages (from-to)794-801
Number of pages8
JournalBiological and Pharmaceutical Bulletin
Volume37
Issue number5
DOIs
Publication statusPublished - 2014 May

Keywords

  • AMP-activated protein kinase (AMPK)
  • Apoptosis
  • MCF-7
  • Rhus verniciflua

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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