Ribosomal protein S3 is stabilized by sumoylation

Chang Young Jang, Hyun Seock Shin, Hag Dong Kim, Jung Woo Kim, Soo Young Choi, Joon Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human ribosomal protein S3 (rpS3) acts as a DNA repair endonuclease. The multiple functions of this protein are regulated by post-translational modifications including phosphorylation and methylation. Using a yeast-two hybrid screen, we identified small ubiquitin-related modifier-1 (SUMO-1) as a new interacting partner of rpS3. rpS3 interacted with SUMO-1 via the N- and C-terminal regions. We also observed sumoylation of rpS3 in Escherichia coli and mammalian cell systems. Furthermore, we discovered that one of three lysine residues, Lys18, Lys214, or Lys230, was sumoylated in rpS3. Interestingly, sumoylated rpS3 was resistant to proteolytic activity, indicating that SUMO-1 increased the stability of the rpS3 protein. We concluded that rpS3 is covalently modified by SUMO-1 and this post-translational modification regulates rpS3 function by increasing rpS3 protein stability.

Original languageEnglish
Pages (from-to)523-527
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume414
Issue number3
DOIs
Publication statusPublished - 2011 Oct 28

Fingerprint

Sumoylation
Ubiquitin
Post Translational Protein Processing
ribosomal protein S3
Phosphorylation
Proteins
Methylation
Protein Stability
Endonucleases
Deoxyribonuclease I
DNA Repair
Yeast
Escherichia coli
Lysine
Repair
Yeasts
Cells

Keywords

  • Protein stability
  • RpS3
  • SUMO-1
  • UBC9

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Ribosomal protein S3 is stabilized by sumoylation. / Jang, Chang Young; Shin, Hyun Seock; Kim, Hag Dong; Kim, Jung Woo; Choi, Soo Young; Kim, Joon.

In: Biochemical and Biophysical Research Communications, Vol. 414, No. 3, 28.10.2011, p. 523-527.

Research output: Contribution to journalArticle

Jang, Chang Young ; Shin, Hyun Seock ; Kim, Hag Dong ; Kim, Jung Woo ; Choi, Soo Young ; Kim, Joon. / Ribosomal protein S3 is stabilized by sumoylation. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 414, No. 3. pp. 523-527.
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