Ribosomal protein S3 localizes on the mitotic spindle and functions as a microtubule associated protein in mitosis

Chang Young Jang, Hag Dong Kim, Xianghua Zhang, Jin Soo Chang, Joon Kim

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The human ribosomal protein S3 (rpS3) has multi-functions such as translation, DNA repair and apoptosis. These multiple functions are regulated by post-translational modifications including phosphorylation, methylation and sumoylation. We report here a novel function of rpS3 that is involved in mitosis. When we examined localization of ribosomal proteins in mitosis, we found that rpS3 specifically localizes on the mitotic spindle. Depletion of the rpS3 proteins caused mitotic arrest during the metaphase. Furthermore, the shape of the spindle and chromosome movement in the rpS3 depleted cell was abnormal. Microtubule (MT) polymerization also decreased in rpS3 depleted cells, suggesting that rpS3 is involved in spindle dynamics. Therefore, we concluded that rpS3 acts as a microtubule associated protein (MAP) and regulates spindle dynamics during mitosis.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume429
Issue number1-2
DOIs
Publication statusPublished - 2012 Dec 7

Fingerprint

Spindle Apparatus
Microtubule-Associated Proteins
Mitosis
Sumoylation
Phosphorylation
Methylation
Ribosomal Proteins
Post Translational Protein Processing
Metaphase
Chromosomes
ribosomal protein S3
Microtubules
DNA Repair
Polymerization
Repair
Apoptosis
DNA

Keywords

  • Chromosome movement
  • Mitotic spindle
  • RpS3
  • Spindle dynamics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Ribosomal protein S3 localizes on the mitotic spindle and functions as a microtubule associated protein in mitosis. / Jang, Chang Young; Kim, Hag Dong; Zhang, Xianghua; Chang, Jin Soo; Kim, Joon.

In: Biochemical and Biophysical Research Communications, Vol. 429, No. 1-2, 07.12.2012, p. 57-62.

Research output: Contribution to journalArticle

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