Ribozyme Mediated Targeting of Cucumber Mosaic Virus RNA 1 and 2 in Transgenic Tobacco Plants

Chang Seob Kwon, Won Il Chung, Kyung Hee Paek

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Hammerhead ribozymes have been extensively used to inhibit the expression of cellular genes or viral genes mainly in the animal study. In this study, we designed a ribozyme targeting the conserved leader sequences of cucumber mosaic virus (CMV) RNA 1 and 2. The ribozyme, with asymmetric lengths of flanking complementary regions, cleaved a model substrate RNA efficiently at 26 °C as well as at 37 °C or 50 °C in vitro. And the ribozyme encoding sequence was introduced into tobacco plants and expressed with the CaMV 35S promoter and 3′ NOS terminator in a monomeric type (pBIR1), tandemly repeated type (pBIR3), and cotranscriptionally combined type (pRokR) with 2.2 copies of I17N satellite RNA. Virus challenging experiments in F1 plants of respective transformants with CMV-Y showed specific reductions of viral RNA 1 and 2 in comparison with RNA 3 or 4. Although young plants of a three-leaf-stage showed rather similar mild symptom attenuations in all constructions compared to CMV-Y inoculated wild type, fully grown plants showed a differential degree of resistance upon systemic infections of CMV-Y in pRokK, pBIR3 and pBIR1 transformed plants in a decreasing order.

Original languageEnglish
Pages (from-to)326-334
Number of pages9
JournalMolecules and cells
Volume7
Issue number3
Publication statusPublished - 1997 Jun 30

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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