Ring-shaped architecture of RecR

Implications for its role in homologous recombinational DNA repair

Byung Lee, Kyoung Hoon Kim, Soo Jeong Park, Soo Hyun Eom, Hyun Kyu Song, Se Won Suh

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

RecR, together with RecF and RecO, facilitates RecA loading in the RecF pathway of homologous recombinational DNA repair in procaryotes. The human Rad52 protein is a functional counterpart of RecFOR. We present here the crystal structure of RecR from Deinococcus radiodurans (DR RecR). A monomer of DR RecR has a two-domain structure: the N-terminal domain with a helix-hairpin-helix (HhH) motif and the C-terminal domain with a Cys 4 zinc-finger motif, a Toprim domain and a Walker B motif. Four such monomers form a ring-shaped tetramer of 222 symmetry with a central hole of 30-35 Å diameter. In the crystal, two tetramers are concatenated, implying that the RecR tetramer is capable of opening and closing. We also show that DR RecR binds to both dsDNA and ssDNA, and that its HhH motif is essential for DNA binding.

Original languageEnglish
Pages (from-to)2029-2038
Number of pages10
JournalEMBO Journal
Volume23
Issue number10
DOIs
Publication statusPublished - 2004 May 19
Externally publishedYes

Fingerprint

Recombinational DNA Repair
Rad52 DNA Repair and Recombination Protein
Repair
Monomers
Deinococcus
DNA
Zinc Fingers
Zinc
Crystal structure
Crystals

Keywords

  • DNA clamp
  • DNA repair
  • Homologous recombination
  • RecF pathway
  • RecR

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Ring-shaped architecture of RecR : Implications for its role in homologous recombinational DNA repair. / Lee, Byung; Kim, Kyoung Hoon; Park, Soo Jeong; Eom, Soo Hyun; Song, Hyun Kyu; Suh, Se Won.

In: EMBO Journal, Vol. 23, No. 10, 19.05.2004, p. 2029-2038.

Research output: Contribution to journalArticle

Lee, Byung ; Kim, Kyoung Hoon ; Park, Soo Jeong ; Eom, Soo Hyun ; Song, Hyun Kyu ; Suh, Se Won. / Ring-shaped architecture of RecR : Implications for its role in homologous recombinational DNA repair. In: EMBO Journal. 2004 ; Vol. 23, No. 10. pp. 2029-2038.
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AU - Suh, Se Won

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