RNA polymerase-associated protein Rap94 confers promoter specificity for initiating transcription of vaccinia virus early stage genes

Byung-Yoon Ahn, P. D. Gershon, B. Moss

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The association of a 94,000-Da viral polypeptide, called Rap94, with 30- 40% of the multisubunit DNA-dependent RNA polymerase molecules purified from infectious vaccinia virus particles was established by immunoaffinity chromatography. The submolar amount of Rap94, relative to RNA polymerase, was confirmed by quantitative immunoblotting of total virion extracts. Only the RNA polymerase molecules containing Rap94 could functionally interact with VETF, the vaccinia virus early transcription factor, to transcribe a double- stranded DNA template regulated by a viral early stage promoter. Rap94 was required for the synthesis of short oligoribonucleotides and for the formation of stable ternary transcription complexes. With a nonspecific single-stranded DNA template, however, the Rap94-deficient polymerase had greater catalytic activity than the Rap94-containing polymerase. These data support a model in which Rap94 confers specificity to the RNA polymerase for promoters of early stage genes.

Original languageEnglish
Pages (from-to)7552-7557
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number10
Publication statusPublished - 1994 Mar 11
Externally publishedYes

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Vaccinia virus
DNA-Directed RNA Polymerases
Transcription
Viruses
Genes
Virion
Proteins
Oligoribonucleotides
Molecules
Single-Stranded DNA
Chromatography
Immunoblotting
Catalyst activity
Transcription Factors
Association reactions
Peptides
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

RNA polymerase-associated protein Rap94 confers promoter specificity for initiating transcription of vaccinia virus early stage genes. / Ahn, Byung-Yoon; Gershon, P. D.; Moss, B.

In: Journal of Biological Chemistry, Vol. 269, No. 10, 11.03.1994, p. 7552-7557.

Research output: Contribution to journalArticle

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