Robust enhancement of neural differentiation from human ES and iPS cells regardless of their innate difference in differentiation propensity

Dae Sung Kim, Jae Souk Lee, Joong Woo Leem, Yong Jun Huh, Ji Young Kim, Han Soo Kim, In Hyun Park, George Q. Daley, Dong Youn Hwang, Dong Wook Kim

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Our analyses of three human induced pluripotent stem cell (hiPSC) and six human embryonic stem cell (hESC) lines showed marked variability in differentiation potential into specific lineages, which often hampers their differentiation into specific cell types or cell lineages of interest. Simultaneous inhibition of both Activin/Nodal and BMP pathways with small molecules, SB431542 and dorsomorphin (DM), respectively, promoted significant neural differentiation from all human pluripotent stem cell (hPSC) lines tested, regardless of their differentiation propensity. On the contrary, differentiation into other cell lineages and the number of undifferentiated cells were significantly reduced after differentiation by the dual inhibition. These results demonstrate that innate differentiation propensity of hPSCs could be overcome, at least in part, by modulation of intracellular signaling pathways, resulting in efficient generation of desirable cell types, such as neural cells.

Original languageEnglish
Pages (from-to)270-281
Number of pages12
JournalStem Cell Reviews and Reports
Volume6
Issue number2
DOIs
Publication statusPublished - 2010 Jun

Keywords

  • Cell signaling
  • Differentiation propensity
  • Neural induction
  • Pluripotent stem cell
  • Small molecule

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

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