Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence

Soon Il Kwon; Hong Joo Cho; Kisuk Bae; Jin Hee Jung; Hak Chul Jin; Ohkmae K. Park

doi:10.1007/s12374-009-9011-4

Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence

Soon Il Kwon, Hong Joo Cho, Kisuk Bae, Jin Hee Jung, Hak Chul Jin, Ohkmae K. Park

Division of Life Sciences

Research output: Contribution to journal › Review article › peer-review

24 Citations (Scopus)

Abstract

In our previous proteomic analysis, we isolated a small GTPase RabG3b as a salicylic acid-responsive protein in Arabidopsis (Oh et al. in Plant Cell 17:2832-2847, 2005). Here, we constructed transgenic plants overexpressing wild-type (RabG3bOX), constitutively active (RabG3bCA), and dominant negative (RabG3bDN) forms of RabG3b for functional studies. The phenotypes of these transgenic plants were indistinguishable from wild-type plants under normal growth conditions. However, both RabG3bOX and RabG3bCA plants displayed unrestricted hypersensitive programmed cell death against a fungal toxin Fumonisin B1 and a fungal pathogen Alternaria brassicicola, whereas no major difference between wildtype and RabG3bDN plants was observed. In addition, RabG3bOX and RabG3bCA plants underwent accelerated leaf senescence compared to wild-type and RabG3bDNplants. These results suggest that RabG3b is a modulator for cell death progression during pathogen response and senescence process in plants.

Original language	English
Pages (from-to)	79-87
Number of pages	9
Journal	Journal of Plant Biology
Volume	52
Issue number	2
DOIs	https://doi.org/10.1007/s12374-009-9011-4
Publication status	Published - 2009 Apr

Keywords

Alternaria brassicicola
Fumonisin B1
Hypersensitive response
Leaf senescence
Programmed cell death
Rab GTPase

ASJC Scopus subject areas

Plant Science

Access to Document

10.1007/s12374-009-9011-4

Fingerprint Dive into the research topics of 'Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence'. Together they form a unique fingerprint.

Cite this

@article{7d97a512394644428028c84943a0fe00,

title = "Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence",

abstract = "In our previous proteomic analysis, we isolated a small GTPase RabG3b as a salicylic acid-responsive protein in Arabidopsis (Oh et al. in Plant Cell 17:2832-2847, 2005). Here, we constructed transgenic plants overexpressing wild-type (RabG3bOX), constitutively active (RabG3bCA), and dominant negative (RabG3bDN) forms of RabG3b for functional studies. The phenotypes of these transgenic plants were indistinguishable from wild-type plants under normal growth conditions. However, both RabG3bOX and RabG3bCA plants displayed unrestricted hypersensitive programmed cell death against a fungal toxin Fumonisin B1 and a fungal pathogen Alternaria brassicicola, whereas no major difference between wildtype and RabG3bDN plants was observed. In addition, RabG3bOX and RabG3bCA plants underwent accelerated leaf senescence compared to wild-type and RabG3bDNplants. These results suggest that RabG3b is a modulator for cell death progression during pathogen response and senescence process in plants.",

keywords = "Alternaria brassicicola, Fumonisin B1, Hypersensitive response, Leaf senescence, Programmed cell death, Rab GTPase",

author = "Kwon, {Soon Il} and Cho, {Hong Joo} and Kisuk Bae and Jung, {Jin Hee} and Jin, {Hak Chul} and Park, {Ohkmae K.}",

note = "Funding Information: Acknowledgments This work was supported by grants from the Plant Signaling Network Research Center (R11-2003-008-04004-0), the Biotechnology Development Program (2006-02762), and the Basic Science Program (R01-2007-000-10548-0) funded by the Korea Science and Engineering Foundation.",

year = "2009",

month = apr,

doi = "10.1007/s12374-009-9011-4",

language = "English",

volume = "52",

pages = "79--87",

journal = "Journal of Plant Biology",

issn = "1226-9239",

publisher = "Springer New York",

number = "2",

}

TY - JOUR

T1 - Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence

AU - Kwon, Soon Il

AU - Cho, Hong Joo

AU - Bae, Kisuk

AU - Jung, Jin Hee

AU - Jin, Hak Chul

AU - Park, Ohkmae K.

N1 - Funding Information: Acknowledgments This work was supported by grants from the Plant Signaling Network Research Center (R11-2003-008-04004-0), the Biotechnology Development Program (2006-02762), and the Basic Science Program (R01-2007-000-10548-0) funded by the Korea Science and Engineering Foundation.

PY - 2009/4

Y1 - 2009/4

N2 - In our previous proteomic analysis, we isolated a small GTPase RabG3b as a salicylic acid-responsive protein in Arabidopsis (Oh et al. in Plant Cell 17:2832-2847, 2005). Here, we constructed transgenic plants overexpressing wild-type (RabG3bOX), constitutively active (RabG3bCA), and dominant negative (RabG3bDN) forms of RabG3b for functional studies. The phenotypes of these transgenic plants were indistinguishable from wild-type plants under normal growth conditions. However, both RabG3bOX and RabG3bCA plants displayed unrestricted hypersensitive programmed cell death against a fungal toxin Fumonisin B1 and a fungal pathogen Alternaria brassicicola, whereas no major difference between wildtype and RabG3bDN plants was observed. In addition, RabG3bOX and RabG3bCA plants underwent accelerated leaf senescence compared to wild-type and RabG3bDNplants. These results suggest that RabG3b is a modulator for cell death progression during pathogen response and senescence process in plants.

AB - In our previous proteomic analysis, we isolated a small GTPase RabG3b as a salicylic acid-responsive protein in Arabidopsis (Oh et al. in Plant Cell 17:2832-2847, 2005). Here, we constructed transgenic plants overexpressing wild-type (RabG3bOX), constitutively active (RabG3bCA), and dominant negative (RabG3bDN) forms of RabG3b for functional studies. The phenotypes of these transgenic plants were indistinguishable from wild-type plants under normal growth conditions. However, both RabG3bOX and RabG3bCA plants displayed unrestricted hypersensitive programmed cell death against a fungal toxin Fumonisin B1 and a fungal pathogen Alternaria brassicicola, whereas no major difference between wildtype and RabG3bDN plants was observed. In addition, RabG3bOX and RabG3bCA plants underwent accelerated leaf senescence compared to wild-type and RabG3bDNplants. These results suggest that RabG3b is a modulator for cell death progression during pathogen response and senescence process in plants.

KW - Alternaria brassicicola

KW - Fumonisin B1

KW - Hypersensitive response

KW - Leaf senescence

KW - Programmed cell death

KW - Rab GTPase

UR - http://www.scopus.com/inward/record.url?scp=70349413411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349413411&partnerID=8YFLogxK

U2 - 10.1007/s12374-009-9011-4

DO - 10.1007/s12374-009-9011-4

M3 - Review article

AN - SCOPUS:70349413411

VL - 52

SP - 79

EP - 87

JO - Journal of Plant Biology

JF - Journal of Plant Biology

SN - 1226-9239

IS - 2

ER -

Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this