Abstract
In our previous proteomic analysis, we isolated a small GTPase RabG3b as a salicylic acid-responsive protein in Arabidopsis (Oh et al. in Plant Cell 17:2832-2847, 2005). Here, we constructed transgenic plants overexpressing wild-type (RabG3bOX), constitutively active (RabG3bCA), and dominant negative (RabG3bDN) forms of RabG3b for functional studies. The phenotypes of these transgenic plants were indistinguishable from wild-type plants under normal growth conditions. However, both RabG3bOX and RabG3bCA plants displayed unrestricted hypersensitive programmed cell death against a fungal toxin Fumonisin B1 and a fungal pathogen Alternaria brassicicola, whereas no major difference between wildtype and RabG3bDN plants was observed. In addition, RabG3bOX and RabG3bCA plants underwent accelerated leaf senescence compared to wild-type and RabG3bDNplants. These results suggest that RabG3b is a modulator for cell death progression during pathogen response and senescence process in plants.
| Original language | English |
|---|---|
| Pages (from-to) | 79-87 |
| Number of pages | 9 |
| Journal | Journal of Plant Biology |
| Volume | 52 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Apr 1 |
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Keywords
- Alternaria brassicicola
- Fumonisin B1
- Hypersensitive response
- Leaf senescence
- Programmed cell death
- Rab GTPase
ASJC Scopus subject areas
- Plant Science
Cite this
Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence. / Kwon, Soon Il; Cho, Hong Joo; Bae, Kisuk; Jung, Jin Hee; Jin, Hak Chul; Kim, Ohkmae.
In: Journal of Plant Biology, Vol. 52, No. 2, 01.04.2009, p. 79-87.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence
AU - Kwon, Soon Il
AU - Cho, Hong Joo
AU - Bae, Kisuk
AU - Jung, Jin Hee
AU - Jin, Hak Chul
AU - Kim, Ohkmae
PY - 2009/4/1
Y1 - 2009/4/1
N2 - In our previous proteomic analysis, we isolated a small GTPase RabG3b as a salicylic acid-responsive protein in Arabidopsis (Oh et al. in Plant Cell 17:2832-2847, 2005). Here, we constructed transgenic plants overexpressing wild-type (RabG3bOX), constitutively active (RabG3bCA), and dominant negative (RabG3bDN) forms of RabG3b for functional studies. The phenotypes of these transgenic plants were indistinguishable from wild-type plants under normal growth conditions. However, both RabG3bOX and RabG3bCA plants displayed unrestricted hypersensitive programmed cell death against a fungal toxin Fumonisin B1 and a fungal pathogen Alternaria brassicicola, whereas no major difference between wildtype and RabG3bDN plants was observed. In addition, RabG3bOX and RabG3bCA plants underwent accelerated leaf senescence compared to wild-type and RabG3bDNplants. These results suggest that RabG3b is a modulator for cell death progression during pathogen response and senescence process in plants.
AB - In our previous proteomic analysis, we isolated a small GTPase RabG3b as a salicylic acid-responsive protein in Arabidopsis (Oh et al. in Plant Cell 17:2832-2847, 2005). Here, we constructed transgenic plants overexpressing wild-type (RabG3bOX), constitutively active (RabG3bCA), and dominant negative (RabG3bDN) forms of RabG3b for functional studies. The phenotypes of these transgenic plants were indistinguishable from wild-type plants under normal growth conditions. However, both RabG3bOX and RabG3bCA plants displayed unrestricted hypersensitive programmed cell death against a fungal toxin Fumonisin B1 and a fungal pathogen Alternaria brassicicola, whereas no major difference between wildtype and RabG3bDN plants was observed. In addition, RabG3bOX and RabG3bCA plants underwent accelerated leaf senescence compared to wild-type and RabG3bDNplants. These results suggest that RabG3b is a modulator for cell death progression during pathogen response and senescence process in plants.
KW - Alternaria brassicicola
KW - Fumonisin B1
KW - Hypersensitive response
KW - Leaf senescence
KW - Programmed cell death
KW - Rab GTPase
UR - http://www.scopus.com/inward/record.url?scp=70349413411&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349413411&partnerID=8YFLogxK
U2 - 10.1007/s12374-009-9011-4
DO - 10.1007/s12374-009-9011-4
M3 - Article
AN - SCOPUS:70349413411
VL - 52
SP - 79
EP - 87
JO - Journal of Plant Biology
JF - Journal of Plant Biology
SN - 1226-9239
IS - 2
ER -