Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence

Soon Il Kwon, Hong Joo Cho, Kisuk Bae, Jin Hee Jung, Hak Chul Jin, Ohkmae Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In our previous proteomic analysis, we isolated a small GTPase RabG3b as a salicylic acid-responsive protein in Arabidopsis (Oh et al. in Plant Cell 17:2832-2847, 2005). Here, we constructed transgenic plants overexpressing wild-type (RabG3bOX), constitutively active (RabG3bCA), and dominant negative (RabG3bDN) forms of RabG3b for functional studies. The phenotypes of these transgenic plants were indistinguishable from wild-type plants under normal growth conditions. However, both RabG3bOX and RabG3bCA plants displayed unrestricted hypersensitive programmed cell death against a fungal toxin Fumonisin B1 and a fungal pathogen Alternaria brassicicola, whereas no major difference between wildtype and RabG3bDN plants was observed. In addition, RabG3bOX and RabG3bCA plants underwent accelerated leaf senescence compared to wild-type and RabG3bDNplants. These results suggest that RabG3b is a modulator for cell death progression during pathogen response and senescence process in plants.

Original languageEnglish
Pages (from-to)79-87
Number of pages9
JournalJournal of Plant Biology
Volume52
Issue number2
DOIs
Publication statusPublished - 2009 Apr 1

Fingerprint

guanosinetriphosphatase
Arabidopsis
pathogens
leaves
transgenic plants
Alternaria brassicicola
fumonisin B1
salicylic acid
proteomics
mycotoxins
cell death
apoptosis
phenotype
proteins
cells

Keywords

  • Alternaria brassicicola
  • Fumonisin B1
  • Hypersensitive response
  • Leaf senescence
  • Programmed cell death
  • Rab GTPase

ASJC Scopus subject areas

  • Plant Science

Cite this

Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence. / Kwon, Soon Il; Cho, Hong Joo; Bae, Kisuk; Jung, Jin Hee; Jin, Hak Chul; Kim, Ohkmae.

In: Journal of Plant Biology, Vol. 52, No. 2, 01.04.2009, p. 79-87.

Research output: Contribution to journalArticle

Kwon, Soon Il ; Cho, Hong Joo ; Bae, Kisuk ; Jung, Jin Hee ; Jin, Hak Chul ; Kim, Ohkmae. / Role of an Arabidopsis rab GTPase RabG3b in pathogen response and leaf senescence. In: Journal of Plant Biology. 2009 ; Vol. 52, No. 2. pp. 79-87.
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