Abstract
The vascular system of plants consists of two conducting tissues, xylem and phloem, which differentiate from procambium cells. Xylem serves as a transporting system for water and signaling molecules and is formed by sequential developmental processes, including cell division/expansion, secondary cell wall deposition, vacuole collapse and programmed cell death (PCD). PCD during xylem differentiation is accomplished by degradation of cytoplasmic constituents, and it is required for the formation of hollow vessels, known as tracheary elements (TEs). Our recent study revealed that the small GTPase RabG3b acts as a regulator of TE differentiation through its autophagic activation. By using an Arabidopsis in vitro cell culture system, we showed that autophagy is activated during TE differentiation. Overexpression of a constitutively active RabG3b (RabG3bCA) significantly enhances both autophagy and TE differentiation, which are consistently suppressed in transgenic plants overexpressing a dominant negative form (RabG3bDN) or RabG3b RNAi (RabG3bRNAi), a brassinosteroid- insensitive mutant bri1-301 and an autophagy mutant atg5-1. On the basis of our results, we propose that RabG3b functions as a component of autophagy and regulates TE differentiation by activating the process of PCD.
| Original language | English |
|---|---|
| Pages (from-to) | 1187-1189 |
| Number of pages | 3 |
| Journal | Autophagy |
| Volume | 6 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2010 Nov 16 |
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Keywords
- Arabidopsis
- Autophagic cell death
- Autophagy
- RabG3b
- Small GTP-binding protein
- Tracheary element differentiation
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Role of Arabidopsis RabG3b and autophagy in tracheary element differentiation. / Kwon, Soon Il; Cho, Hong Joo; Kim, Ohkmae.
In: Autophagy, Vol. 6, No. 8, 16.11.2010, p. 1187-1189.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of Arabidopsis RabG3b and autophagy in tracheary element differentiation
AU - Kwon, Soon Il
AU - Cho, Hong Joo
AU - Kim, Ohkmae
PY - 2010/11/16
Y1 - 2010/11/16
N2 - The vascular system of plants consists of two conducting tissues, xylem and phloem, which differentiate from procambium cells. Xylem serves as a transporting system for water and signaling molecules and is formed by sequential developmental processes, including cell division/expansion, secondary cell wall deposition, vacuole collapse and programmed cell death (PCD). PCD during xylem differentiation is accomplished by degradation of cytoplasmic constituents, and it is required for the formation of hollow vessels, known as tracheary elements (TEs). Our recent study revealed that the small GTPase RabG3b acts as a regulator of TE differentiation through its autophagic activation. By using an Arabidopsis in vitro cell culture system, we showed that autophagy is activated during TE differentiation. Overexpression of a constitutively active RabG3b (RabG3bCA) significantly enhances both autophagy and TE differentiation, which are consistently suppressed in transgenic plants overexpressing a dominant negative form (RabG3bDN) or RabG3b RNAi (RabG3bRNAi), a brassinosteroid- insensitive mutant bri1-301 and an autophagy mutant atg5-1. On the basis of our results, we propose that RabG3b functions as a component of autophagy and regulates TE differentiation by activating the process of PCD.
AB - The vascular system of plants consists of two conducting tissues, xylem and phloem, which differentiate from procambium cells. Xylem serves as a transporting system for water and signaling molecules and is formed by sequential developmental processes, including cell division/expansion, secondary cell wall deposition, vacuole collapse and programmed cell death (PCD). PCD during xylem differentiation is accomplished by degradation of cytoplasmic constituents, and it is required for the formation of hollow vessels, known as tracheary elements (TEs). Our recent study revealed that the small GTPase RabG3b acts as a regulator of TE differentiation through its autophagic activation. By using an Arabidopsis in vitro cell culture system, we showed that autophagy is activated during TE differentiation. Overexpression of a constitutively active RabG3b (RabG3bCA) significantly enhances both autophagy and TE differentiation, which are consistently suppressed in transgenic plants overexpressing a dominant negative form (RabG3bDN) or RabG3b RNAi (RabG3bRNAi), a brassinosteroid- insensitive mutant bri1-301 and an autophagy mutant atg5-1. On the basis of our results, we propose that RabG3b functions as a component of autophagy and regulates TE differentiation by activating the process of PCD.
KW - Arabidopsis
KW - Autophagic cell death
KW - Autophagy
KW - RabG3b
KW - Small GTP-binding protein
KW - Tracheary element differentiation
UR - http://www.scopus.com/inward/record.url?scp=78649254014&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78649254014&partnerID=8YFLogxK
U2 - 10.4161/auto.6.8.13429
DO - 10.4161/auto.6.8.13429
M3 - Article
VL - 6
SP - 1187
EP - 1189
JO - Autophagy
JF - Autophagy
SN - 1554-8627
IS - 8
ER -