Role of group 3 innate lymphoid cells during experimental otitis media in a rat model

Chang Gun Cho, Sung Ho Gong, Hee Bok Kim, Jae-Jun Song, Joo Hyun Park, Yun Sung Lim, Seok Won Park

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The objective of this study was to evaluate the role of group 3 innate lymphoid cells (ILC3) in the middle ear (ME) mucosal response to bacterial infection in a rat model. To confirm the role of ILC3 in bacterially induced otitis media (OM), the serum concentrations of IL-17 and IL-22 were determined by ELISA, and the tissue expression of IL-17 and IL-22 in infected ME mucosa was assessed by immunohistochemical staining. Immunohistochemical staining of specific cell surface markers was also assessed to confirm the origin of the cells expressing IL-17 and IL-22. Twenty Sprague-Dawley rats were used in the surgically-induced animal model of OM. OM was induced by inoculation of non-typeable Haemophilus influenzae into the ME cavity of the rats. The rats were divided into four experimental groups: three infected groups and one control group. Infected groups were subdivided into sets of 5 rats, one for each of the three time points (1, 4 and 7 days post-inoculation). For determination of rat IL-17 and IL-22 levels in infected rats and control rats, infected or control ME mucosa sections were analyzed by immunohistochemistry with specific antibodies directed against IL-17 and IL-22. Immunohistochemical staining for CD3, RORγt, and NKp46 were also conducted on the samples to confirm the origin of cells expressing IL-17 and IL-22. IL-17 and IL-22 serum concentrations were significantly increased in the infected rats compared to control rats. Immunohistochemical staining revealed increased IL-17 and IL-22 expressions in all infected ME mucosae from the first day after inoculation. In addition, the results of tissue staining for the specific surface markers were negative for CD3 and NKp46, but were highly positive for RORγt. IL-17 and IL-22 revealed their association with the bacterially induced proliferative and hyperplastic responses of ME mucosa, which are characteristic features in pathogenesis of OM. Surface marker examination showed that the source cells for IL-17 and IL-22 seemed to be lymphoid tissue inducer (LTi) cells. The results suggest that LTi cells release IL-17 and IL-22, and play a significant role in both the early phase of OM induction and recovery from it.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume88
DOIs
Publication statusPublished - 2016 Sep 1

Fingerprint

Interleukin-17
Otitis Media
Lymphocytes
Middle Ear
Staining and Labeling
Mucous Membrane
Lymphoid Tissue
Helper-Inducer T-Lymphocytes
interleukin-22
Haemophilus influenzae
Serum
Bacterial Infections
Sprague Dawley Rats
Animal Models
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry

Keywords

  • Haemophilus influenzae
  • Innate immunity
  • Interleukin-17
  • Interleukin-22
  • Mucosal immunity
  • Otitis media

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Otorhinolaryngology

Cite this

Role of group 3 innate lymphoid cells during experimental otitis media in a rat model. / Cho, Chang Gun; Gong, Sung Ho; Kim, Hee Bok; Song, Jae-Jun; Park, Joo Hyun; Lim, Yun Sung; Park, Seok Won.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 88, 01.09.2016, p. 146-152.

Research output: Contribution to journalArticle

Cho, Chang Gun ; Gong, Sung Ho ; Kim, Hee Bok ; Song, Jae-Jun ; Park, Joo Hyun ; Lim, Yun Sung ; Park, Seok Won. / Role of group 3 innate lymphoid cells during experimental otitis media in a rat model. In: International Journal of Pediatric Otorhinolaryngology. 2016 ; Vol. 88. pp. 146-152.
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