Role of Helicobacter pylori plasticity region genes in development of gastroduodenal diseases

Mitsushige Sugimoto, Masahide Watada, Sung Woo Jung, David Y. Graham, Yoshio Yamaoka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The plasticity region of Helicobacter pylori is a large chromosomal segment including isolate-specific open reading frames with characteristics of pathogenicity islands. It remains unclear whether genes in the plasticity region play a role in the pathogenesis of gastric mucosal inflammation and gastroduodenal disease. Our aim was to assess the role of selected genes in the plasticity region in relation to risk of H. pylori-related disease and the severity of gastric mucosal damage. We used PCR to study the relation of disease outcome and mucosal damage with four genes in the H. pylori plasticity region (jhp0940, jhp0945, jhp0947, and jhp0949) from isolates obtained from both Western (n=296) and East Asian (n=217) patients. The prevalence of jhp0945, jhp0947, and jhp0949 differed significantly between Western and East Asian isolates. In Western isolates, the presence of jhp0945 was significantly associated with gastric ulcer, duodenal ulcer, and gastric cancer (odds ratios [95% confidence intervals]: 2.27 [1.04 to 4.98], 1.86 [1.03 to 3.34], and 1.92 [1.03 to 3.56], respectively). jhp0940-positive Western isolates were significantly associated with absence of gastric ulcer or duodenal ulcer (0.21 [0.05 to 0.94] and 0.31 [0.12 to 0.78], respectively). No significant difference was observed between inflammatory cell infiltration or atrophy and the presence or absence of plasticity region genes. The outcome of H. pylori infections varies widely geographically. These data suggest a possible role for difference in the prevalence of plasticity region genes in the geographic variation in H. pylori-related diseases.

Original languageEnglish
Pages (from-to)441-448
Number of pages8
JournalJournal of Clinical Microbiology
Volume50
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

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Helicobacter pylori
Genes
Stomach Ulcer
Duodenal Ulcer
Duodenal Neoplasms
Genomic Islands
Stomach Diseases
Helicobacter Infections
Open Reading Frames
Stomach Neoplasms
Atrophy
Stomach
Odds Ratio
Confidence Intervals
Inflammation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Role of Helicobacter pylori plasticity region genes in development of gastroduodenal diseases. / Sugimoto, Mitsushige; Watada, Masahide; Jung, Sung Woo; Graham, David Y.; Yamaoka, Yoshio.

In: Journal of Clinical Microbiology, Vol. 50, No. 2, 01.02.2012, p. 441-448.

Research output: Contribution to journalArticle

Sugimoto, Mitsushige ; Watada, Masahide ; Jung, Sung Woo ; Graham, David Y. ; Yamaoka, Yoshio. / Role of Helicobacter pylori plasticity region genes in development of gastroduodenal diseases. In: Journal of Clinical Microbiology. 2012 ; Vol. 50, No. 2. pp. 441-448.
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